Biometric measurement of the mouse eye using optical coherence tomography with focal plane advancement

Zhou, Xiangtian; Xie, Jing; Shen, Meixiao; Wang, Jianhua; Jiang, Liqin; Qu, Jia; Liu, Fan

doi:10.1016/j.visres.2008.01.030

Cited by 63 publications

(47 citation statements)

References 15 publications

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“…9,15,42,45 This difference may be a critical factor because there are numerous reports indicating that laboratory light intensity and duration are in fact contributing factors because they do affect FDM progression in animal studies. 6,8,[36][37][38] Our results agree other reports indicating that the intensity and duration of light exposure are contributing factors to FDM in mice.…”

Section: D1r (At Least Partially) Mediates Bl Inhibitory Effects On Rsupporting

confidence: 91%

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Bright Light Suppresses Form-Deprivation Myopia Development With Activation of Dopamine D1 Receptor Signaling in the ON Pathway in Retina

Chen

Zhi

Ruan

et al. 2017

Invest. Ophthalmol. Vis. Sci.

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Citation: Chen S, Zhi Z, Ruan Q, et al. Bright light suppresses form-deprivation myopia development with activation of dopamine D1 receptor signaling in the ON pathway in retina. Invest Ophthalmol Vis Sci. 2017;58:230658: -231658: . DOI:10.1167 PURPOSE. To determine whether dopamine receptor D1 (D1R) signaling pathway activation by bright light (BL) in specific retinal neuronal cell types contributes to inhibiting formdeprivation myopia (FDM) in mice.METHODS. Mice (3-weeks old) were raised under either normal light (NL: 100-200 lux) or BL (2500-5000 lux) conditions with or without form deprivation. Refraction changes were evaluated with an eccentric infrared photorefractor, and ocular axial components with optical coherence tomography. The D1R antagonist, SCH39166, was intraperitoneally injected daily to evaluate if BL mediates declines in FDM development through D1R activation. Six different biomarkers of retinal neuronal types delineated differential distribution of D1R expression. cFos and phosphorylated tyrosine hydroxylase (p-TH) immunofluorescent staining evaluated D1R receptor activation and dopamine synthesis, respectively. CONCLUSIONS. Bright light increases D1R activity in the BCs of the ON pathway, which is associated with less myopic shift and ocular elongation than those occurring in NL. These declines suggest that increased D1R activity in the ON pathway contributes to the BL suppression of FDM development in mice.

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Section: D1r (At Least Partially) Mediates Bl Inhibitory Effects On Rsupporting

confidence: 91%

“…They were measured as described using a custom-made optical coherence tomography (OCT). 37 Mice were intraperitoneally anesthetized with a mixture of 0.12 g/mL ketamine hydrochloride and 1.8 mg/mL xylazine hydrochloride before the measurement. The injection volume was 8 mL/kg of body weight.…”

Section: Biometric Measurementsmentioning

confidence: 99%

Bright Light Suppresses Form-Deprivation Myopia Development With Activation of Dopamine D1 Receptor Signaling in the ON Pathway in Retina

Chen

Zhi

Ruan

et al. 2017

Invest. Ophthalmol. Vis. Sci.

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“…55 Recently, Zhou et al developed a custom-built real-time optical-coherence tomography (OCT) instrument with a stepper motor, which has been proven to be highly repeatable in measuring individual parts of the mouse eye, including the cornea, anterior chamber, lens, vitreous, and retina. 56 They also successfully used a custombuilt photoretinoscope to measure refraction and a keratometer to measure the corneal radius of curvature. 52 These new techniques could be used in further study.…”

Section: Shh Expression and Its Role In Fdm In Micementioning

confidence: 99%

Sonic Hedgehog Expression and Its Role in Form-Deprivation Myopia in Mice

Qian

Chu

et al. 2009

Current Eye Research

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“…20 It offers the possibility of whole eyeball imaging in rodents. 25 This study aims to assess lens regeneration after extracapsular lens extraction (ECLE) in vivo by UL-OCT, and explore the iovs.arvojournals.org j ISSN: 1552-5783 pattern of morphologic changes of the process by continuous observation.…”

Section: Methodsmentioning

confidence: 99%

In Vivo Observation of Lens Regeneration in Rat Using Ultra-Long Scan Depth Optical Coherence Tomography

Zhou

Huang³

et al. 2016

Invest. Ophthalmol. Vis. Sci.

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In vivo observation of lens regeneration in rat using ultralong scan depth optical coherence tomography. Invest Ophthalmol Vis Sci. 2016;57:6615-6623. DOI: 10.1167/iovs.16-19363 PURPOSE. To evaluate morphologic changes of lens regeneration in rats in vivo after extracapsular lens extraction (ECLE) by ultra-long scan depth optical coherence tomography (UL-OCT). METHODS.A total of 42 Sprague-Dawley rats were used in this study. We performed ECLE on the right eyes of animals in the surgery group (n ¼ 34). Biomicroscopy and UL-OCT scans were carried out for the surgery group immediately (within 1 hour postoperatively) and at days 1 and 3, weeks 1 and 2, and months 1, 2, and 3 postoperatively. After in vivo examination, three animals of the surgery group were euthanized at each time point for histology study, while the other 10 animals were examined continuously at those time points. The regenerated lens was evaluated in OCT images at 2 and 3 months postoperatively. The control group consisted of eight untreated rats that had OCT examination at the age of 5 months.RESULTS. Lens regeneration could be observed from 2 weeks postoperatively. Regeneration was mainly at the peripheral capsular bag in the first month and central region thereafter. The average thickness of regenerated lenses was 2222 6 309 and 2324 6 352 lm at 2 and 3 months, respectively. Regeneration was faster in the first 2 months and slowed down thereafter. Although anterior capsule opening and posterior capsule adhesion and wrinkling existed, the regenerated lens still could form a relatively regular shape, however, the size was much smaller than that of the normal lenses from rats with the same age.CONCLUSIONS. Ultra-long OCT provides longitudinal data of the process of lens regeneration on a single individual rat in vivo, which may allow one to follow and compare the lens regenerative process under different interventions or therapy after ECLE in rats.

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Biometric measurement of the mouse eye using optical coherence tomography with focal plane advancement

Abstract: By focal plane advancement of a real-time OCT instrument through the mouse eye, highly repeatable measurements of the ocular dimensions were obtained. This novel method may be used to study small animal models of normal and abnormal eye development.

Cited by 63 publications

References 15 publications

Bright Light Suppresses Form-Deprivation Myopia Development With Activation of Dopamine D1 Receptor Signaling in the ON Pathway in Retina

Bright Light Suppresses Form-Deprivation Myopia Development With Activation of Dopamine D1 Receptor Signaling in the ON Pathway in Retina

Sonic Hedgehog Expression and Its Role in Form-Deprivation Myopia in Mice

In Vivo Observation of Lens Regeneration in Rat Using Ultra-Long Scan Depth Optical Coherence Tomography

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