Biomembrane models

Wiśniewska-Becker, Anna; Gruszecki, Wiesław I.

doi:10.1533/9781908818348.47

Cited by 7 publications

(3 citation statements)

References 124 publications

(151 reference statements)

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“…However, the processes involved in the interaction between a drug and biomembranes are very complex, and biomembranes are very complicated structures; as a consequence, interactions between drug and biomembrane are difficult to study and understand. For this reason, biomimetic model cell membranes, such as liposomes and phospholipid monolayers, are widely used to study such interactions [ 1 , 2 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Protocatechuic Acid Ethyl Ester on Biomembrane Models: Multilamellar Vesicles and Monolayers

et al. 2022

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The interactions of drugs with cell membranes are of primary importance for several processes involved in drugs activity. However, these interactions are very difficult to study due to the complexity of biological membranes. Lipid model membranes have been developed and used to gain insight into drug–membrane interactions. In this study, the interaction of protocatechuic acid ethyl ester, showing radical-scavenging activity, antimicrobial, antitumor and anti-inflammatory effects, with model membranes constituted by multilamellar vesicles and monolayers made of DMPC and DSPC, has been studied. Differential scanning calorimetry and Langmuir–Blodgett techniques have been used. Protocatechuic acid ethyl ester interacted both with MLV and monolayers. However, a stronger interaction of the drug with DMPC-based model membranes has been obtained. The finding of this study could help to understand the protocatechuic acid ethyl ester action mechanism.

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Section: Introductionmentioning

confidence: 99%

Effect of Protocatechuic Acid Ethyl Ester on Biomembrane Models: Multilamellar Vesicles and Monolayers

et al. 2022

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“…Nanodiscs are engineered based on apolipoprotein ApoA I, a component of high-density lipoprotein. − Primary reasons for the development of nanodiscs are challenges related to the studies of membrane proteins (MPs), which require a hydrophobic environment naturally ensured by lipids. The use of nanodiscs as a platform to study MPs seems to be an excellent alternative to commonly used micelles, bicelles, and liposomes, since all of them have some disadvantages . Micelles do not have the bilayer structure and bicelles have a bilayer-like arrangement, but they require a high concentration of lipids and detergents.…”

Section: Introductionmentioning

confidence: 99%

“…The use of nanodiscs as a platform to study MPs seems to be an excellent alternative to commonly used micelles, bicelles, and liposomes, 5 since all of them have some disadvantages. 6 Micelles do not have the bilayer structure and bicelles have a bilayer-like arrangement, but they require a high concentration of lipids and detergents. Liposomes are the most suitable systems in structural investigations; however, they are large, unstable, and challenging to prepare.…”

Section: ■ Introductionmentioning

confidence: 99%

Cholesteryl Hemisuccinate Is Not a Good Replacement for Cholesterol in Lipid Nanodiscs

Augustyn¹,

Stępień

Poojari

et al. 2019

J. Phys. Chem. B

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Nanodiscs are suitable tool for studies of membrane proteins (MPs) due to their ability to mimic native biological membranes and several MP structures are solved in nanodiscs. Among the various cell membrane components, cholesterol (CHL) is known to regulate protein function and their concentration can reach up to 50 mol %. However, studies comprising cholesterol is challenging due to its hydrophobic nature, hence, nanodiscs with only low cholesterol concentration has been studied. To overcome the problem, cholesterol analogs with high solubility in polar solutions are often used and one of them is cholesteryl hemisuccinate (CHS). Nevertheless, in molecular dynamics (MD) simulation, this is not an obstacle. In this study, we performed MD simulations of nanodiscs containing neutral phosphatidylcholine (POPC) lipids, negatively charged phosphatidylglycerol (POPG) lipids, CHL, or negatively charged cholesterol analog, CHS. Our simulations show that CHS increases the order of lipids in nanodiscs, the effect is however weaker than CHL, and even smaller in nanodiscs. Furthermore, CHS gathered around scaffold proteins while cholesterol was uniformly distributed in the nanodiscs. Thus, nanodiscs with CHS are heterogeneous and not equivalent to nanodiscs with CHL. Finally, we also observed the increased concentration of POPG near the scaffold proteins, driven by electrostatic interactions. The MD results are experimentally validated using electron paramagnetic resonance spectroscopy. These results show that nanodiscs are in fact, complex structures not easily comparable with planar lipid bilayers.

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Polymer Nanodiscs and Their Bioanalytical Potential

Farrelly

Martin

Thang

2021

Chemistry A European J

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Membrane proteins (MPs) play a pivotal role in cellular function and are therefore predominant pharmaceutical targets. Although detailed understanding of MP structure and mechanistic activity is invaluable for rational drug design, challenges are associated with the purification and study of MPs. This review delves into the historical developments that became the prelude to currently available membrane mimetic technologies before shining a spotlight on polymer nanodiscs. These are soluble nanosized particles capable of encompassing MPs embedded in a phospholipid ring. The expanding range of reported amphipathic polymer nanodisc materials is presented and discussed in terms of their tolerance to different solution conditions and their nanodisc properties. Finally, the analytical scope of polymer nanodiscs is considered in both the demonstration of basic nanodisc parameters as well as in the elucidation of structures, lipidprotein interactions, and the functional mechanisms of reconstituted membrane proteins. The final emphasis is given to the unique benefits and applications demonstrated for native nanodiscs accessed through a detergent free process.

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Biomembrane models

Cited by 7 publications

References 124 publications

Effect of Protocatechuic Acid Ethyl Ester on Biomembrane Models: Multilamellar Vesicles and Monolayers

Effect of Protocatechuic Acid Ethyl Ester on Biomembrane Models: Multilamellar Vesicles and Monolayers

Cholesteryl Hemisuccinate Is Not a Good Replacement for Cholesterol in Lipid Nanodiscs

Polymer Nanodiscs and Their Bioanalytical Potential

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