Biomedical Aspects of Lichen‐Derived Products

Gupta, Anjali; Agarwal, Pooja; Khatana, Kavita; Khan, Shafat Ahmad

doi:10.1002/9781119593249.ch5

Cited by 1 publication

(2 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a result, NBD is likely to trigger LasR complex dissociation during MD simulation since it did not make any hydrophobic interactions with these three amino acid residues. We believe that the new structural and biochemical findings presented herein shed some light into the mechanism of the previously reported antibiofilm activity of Parmotrema lichens [46,47], and will aid in the development of more potent LasR modulators in the future.…”

Section: Discussionmentioning

confidence: 65%

See 1 more Smart Citation

Norlobaridone Inhibits Quorum Sensing-Dependent Biofilm Formation and Some Virulence Factors in Pseudomonas aeruginosa by Disrupting Its Transcriptional Activator Protein LasR Dimerization

Soltane,

Alasiri,

Taha

et al. 2023

Biomolecules

View full text Add to dashboard Cite

In the present study, norlobaridone (NBD) was isolated from Parmotrema and then evaluated as a new potent quorum sensing (QS) inhibitor against Pseudomonas aeruginosa biofilm development. This phenolic natural product was found to reduce P. aeruginosa biofilm formation (64.6% inhibition) and its related virulence factors, such as pyocyanin and rhamnolipids (% inhibition = 61.1% and 55%, respectively). In vitro assays inhibitory effects against a number of known LuxR-type receptors revealed that NBD was able to specifically block P. aeruginosa’s LasR in a dose-dependent manner. Further molecular studies (e.g., sedimentation velocity and thermal shift assays) demonstrated that NBD destabilized LasR upon binding and damaged its functional quaternary structure (i.e., the functional dimeric form). The use of modelling and molecular dynamics (MD) simulations also allowed us to further understand its interaction with LasR, and how this can disrupt its dimeric form. Finally, our findings show that NBD is a powerful and specific LasR antagonist that should be widely employed as a chemical probe in QS of P. aeruginosa, providing new insights into LasR antagonism processes. The new discoveries shed light on the mysterious world of LuxR-type QS in this key opportunistic pathogen.

show abstract

Section: Discussionmentioning

confidence: 65%

“…The genus Parmotrema is one of the widespread lichens that have been used in many countries as an excellent source of multivitamins and bioactive phytochemicals [45]. In addition, several Parmotrema species have also been reported for their broad spectrum antibiofilm and antibacterial potential [46,47].…”

Section: Introductionmentioning

confidence: 99%