Biomedical Applications of Artificial Oxygen Carriers

Waschke, Klaus F.; Lenz, Christian

doi:10.1159/000053536

Cited by 2 publications

(1 citation statement)

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“…Consequently, O 2 that is captured by PFCs can be easily released again, according to a linear relationship with the O 2 partial pressure (PO 2 ) gradient. [12][13][14][15][16] Perfluorodecalin (PFD) is an example of PFC. It is an odorless and colorless high-density liquid with a remarkable capacity to dissolve high amounts of O 2 (up to 50-60 vol%).…”

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Intrarectal administration of oxygenated perfluorodecalin promotes healing of murine colitis by targeting inflammatory hypoxia

Hindryckx

Devisscher

Laukens

et al. 2011

Laboratory Investigation

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Intestinal inflammation is associated with enhanced mucosal hypoxia, which contributes to the ongoing inflammatory process and hampers appropriate mucosal healing. We questioned whether local treatment with an oxygen (O 2 )-carrying and -releasing molecule (oxygenated perfluorodecalin, O 2 -PFD) could positively influence the course of experimental colitis. The impact of intrarectal (IR) treatment with O 2 -PFD was tested using the murine dextran sodium sulfate (DSS)-induced model of distal colitis, both in preventive and therapeutic settings. Colonic mucosal hypoxia was visualized by pimonidazole staining. Colonic permeability was evaluated with FITC-dextran. In the preventive study, mice treated with O 2 -PFD were protected against DSS colitis compared with saline-treated mice, as demonstrated by reduced shortening of colon length, reduced colonic tumor necrosis factor-alpha levels and a lower histological inflammation score (Po0.05 for all parameters). In the therapeutic study, administration of O 2 -PFD resulted in accelerated recovery of colitis compared with saline-treated littermates, and this was reflected by a better weight evolution, lower myeloperoxidase activity and a lower histological inflammation score (Po0.05 for all parameters). It was found that O 2 -PFD established its therapeutic effects through (1) intrinsic anti-inflammatory effects of the PFD molecule and (2) O 2 -induced preservation and healing of the intestinal epithelial surface. Further in vitro and in vivo studies showed that the barrier-protective activity of O 2 -PFD was obtained through prevention of colonocyte apoptosis and stimulation of colonocyte proliferation during inflammatory hypoxia. These data show that IR treatment with O 2 -PFD promotes colitis healing by the combined actions of direct anti-inflammatory effects and O 2 -induced restitution of the epithelial barrier. As such, O 2 -PFD enemas could be an attractive treatment option for patients with distal inflammatory bowel disease.

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