2016
DOI: 10.1038/srep37217
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Biomechanics of subcellular structures by non-invasive Brillouin microscopy

Abstract: Cellular biomechanics play a pivotal role in the pathophysiology of several diseases. Unfortunately, current methods to measure biomechanical properties are invasive and mostly limited to the surface of a cell. As a result, the mechanical behaviour of subcellular structures and organelles remains poorly characterised. Here, we show three-dimensional biomechanical images of single cells obtained with non-invasive, non-destructive Brillouin microscopy with an unprecedented spatial resolution. Our results quantif… Show more

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Cited by 121 publications
(119 citation statements)
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“…The overall elastic stiffness of retinal tissue measured in this study was comparable to that of other CNS tissues assessed by AFM (6, 811, 47, 5052). Similarly, the Brillouin shifts we measured in the retina are comparable with values published in previous literature (18, 20, 21). …”
Section: Discussionsupporting
confidence: 91%
See 1 more Smart Citation
“…The overall elastic stiffness of retinal tissue measured in this study was comparable to that of other CNS tissues assessed by AFM (6, 811, 47, 5052). Similarly, the Brillouin shifts we measured in the retina are comparable with values published in previous literature (18, 20, 21). …”
Section: Discussionsupporting
confidence: 91%
“…We used two different ruminant species for our experiments, sheep and cows. The morphological structure of ovine and bovine retinae is very similar (21) (figure 1), suggesting that their mechanical properties are also likely similar.…”
Section: Discussionmentioning
confidence: 95%
“…These specifications are met by gas lasers and solid-state lasers typically abundant in the blue/green region of the spectrum while semiconductor lasers, often used in the near-infrared region, usually present side modes and noise from amplified spontaneous emission. Indeed, Brillouin studies so far have used frequency doubled solid-state lasers, most using 532 nm wavelength of Nd:YAG laser, some using 561 nm [9,27,28] and one using 671 nm [29]. For in vivo studies, where photodamage is a strict concern, Brillouin studies have used near infrared wavelength (780 nm) employing semiconductor lasers and additional spectral purification elements [13,[30][31][32].…”
Section: Introductionmentioning
confidence: 99%
“…Furthermore, it does not require artificial labelling nor external loading of the sample, thus making it a promising label-free and non-contact method to study mechanical properties of living tissues. In recent years, Brillouin microscopy has enabled applications in cell biology [12][13][14], ophthalmology [15,16], as well as disease detection [17]. Previous works have established that it is in principle feasible to measure cell mechanical properties with subcellular resolution [12,13].…”
Section: Introductionmentioning
confidence: 99%
“…In recent years, Brillouin microscopy has enabled applications in cell biology [12][13][14], ophthalmology [15,16], as well as disease detection [17]. Previous works have established that it is in principle feasible to measure cell mechanical properties with subcellular resolution [12,13]. Furthermore, Schlüssler et al have used Brillouin microscopy for the biomechanical assessment of regenerating tissues in living zebrafish [18] and Palombo et al have studied the contribution to the Brillouin shift of different ECMs from various rat fibrous connective tissue ex-vivo as well as their relative fiber alignment [19].…”
Section: Introductionmentioning
confidence: 99%