Biomechanics of cultured hepatic cells during different steatogenic hits

Baldini, Francesca; Bartolozzi, Alice; Ardito, Martina; Voci, Adriana; Portincasa, Piero; Vassalli, Massimo; Vergani, Laura

doi:10.1016/j.jmbbm.2019.05.036

Cited by 23 publications

(26 citation statements)

References 38 publications

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“…Many models have been proposed to interpret force-indentation curves, accounting for a variety of mechanical components. However, when carefully controlling the experimental parameters it is reasonable to neglect the viscous contribution and obtain a robust description of cell mechanics in terms of a single stiffness parameter [29,31,36]. The simplest and most adopted approach consists of treating the cell as a homogeneous and isotropic material described by the Young's modulus E which is calculated from single cell indentation curves.…”

Section: Resultsmentioning

confidence: 99%

“…HEK-293T cells have been widely characterized in literature, and a broad range of values has been declared, from 300-400Pa [55,56] up to few kPa [57,58]. This issue in comparing experiments obtained in different conditions has been recently challenged [59], and it is suggested that changes in the mechanical properties -as measured in the exact same conditionsare more relevant than absolute values [31]. Nevertheless, the values obtained using the ES approach (350-550Pa, see Fig.5b) lie within the range of published values.…”

Section: Characterizing the Actin Cortex With The Bilayer Decay Modelmentioning

confidence: 99%

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Elasticity Spectra as a Tool to Investigate Actin Cortex Mechanics

Lüchtefeld

Bartolozzi

Morales

et al. 2020

Preprint

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Background The mechanical properties of single living cells have proven to be a powerful marker of the cell physiological state. The use of nanoindentation-based single cell force spectroscopy provided a wealth of information on the elasticity of cells, which is still largely to be exploited. The simplest model to describe cell mechanics is to treat them as a homogeneous elastic material and describe it in terms of the Young's modulus. Beside its simplicity, this approach proved to be extremely informative, allowing to assess the potential of this physical indicator towards high throughput phenotyping in diagnostic and prognostic applications. Results Here we propose an extension of this analysis to explicitly account for the properties of the actin cortex. We present a method, the Elasticity Spectra, to calculate the apparent stiffness of the cell as a function of the indentation depth and we suggest a simple phenomenological approach to measure the thickness and stiffness of the actin cortex, in addition to the standard Young's modulus. Conclusions The Elasticity Spectra approach is tested and validated on a set of cells treated with cytoskeleton-affecting drugs, showing the potential to extend the current representation of cell mechanics, without introducing a detailed and complex description of the intracellular structure.

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Section: Resultsmentioning

confidence: 99%

Section: Characterizing the Actin Cortex With The Bilayer Decay Modelmentioning

confidence: 99%

Elasticity Spectra as a Tool to Investigate Actin Cortex Mechanics

Lüchtefeld

Bartolozzi

Morales

et al. 2020

Preprint

Self Cite

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“…Single-cell mechanics was evaluated using a nanoindentation device (Chiaro, Optics11, Amsterdam, NL) mounted on top of an inverted phase contrast microscope (Evos XL Core, Thermofisher, Paisley, UK) following a previously described approach 55 . hMSCs (Promocell, passage 2) were left to incubate for 72 h at 37 °C and 5% CO2 with the corresponding media (basal media, osteogenic media, basal media with metabolites).…”

Section: Single Cell Force Spectroscopy (Scfs) Experimental Approachmentioning

confidence: 99%

The use of nanovibration to discover specific and potent bioactive metabolites that stimulate osteogenic differentiation in mesenchymal stem cells

Hodgkinson

Tsimbouri

Llopis-Hernández

et al. 2020

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Figure 2. Metabolic profiles of MSCs in 3D culture for 7 days with osteogenic media or nanovibration. (a) Heatmap (red = up-regulations, blue = down-regulations)and (b) principle component analysis depicting differences in nanovibration and OGM metabolic profiles during MSC differentiation. Metabolic profiles of MSCs induced by nanovibrational stimulation show a greater similarity to unstimulated controls at day 7 of culture and to OGM-stimulated cells at day 14. (c) Metabolic profiles were compared by Ingenuity Pathway Analysis (IPA). The metabolic profiles of nanovibrated cells lead to a stronger predicted activation of ERK1/2 relative to the metabolic profiles of cells induced by OGM. In contrast, the metabolic profiles of OGMstimulated cells lead to a stronger predicted down regulation of JNK, while those of nanovibrated cells predict JNK's activation rather than inhibition. Networks are built from d=1, r=4.

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“…HEK-293T cells have been widely characterized in literature, and a broad range of values has been declared, from 300-400Pa [58,59] up to few kPa [60,61]. This issue in comparing experiments obtained in different conditions has been recently challenged [62], and it is suggested that changes in the mechanical properties -as measured in the exact same conditionsare more relevant than absolute values [31]. Nevertheless, the values obtained using the ES approach (350-550Pa, see Fig.5b) lie within the range of published values.…”

Section: Characterizing the Actin Cortex With The Bilayer Decay Modelmentioning

confidence: 99%

Elasticity Spectra as a Tool to Investigate Actin Cortex Mechanics

Lüchtefeld

Bartolozzi

Morales

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background: The mechanical properties of single living cells have proven to be a powerful marker of the cell physiological state. The use of nanoindentation-based single cell force spectroscopy provided a wealth of information on the elasticity of cells, which is still largely to be exploited. The simplest model to describe cell mechanics is to treat them as a homogeneous elastic material and describe it in terms of the Young's modulus. Beside its simplicity, this approach proved to be extremely informative, allowing to assess the potential of this physical indicator towards high throughput phenotyping in diagnostic and prognostic applications. Results: Here we propose an extension of this analysis to explicitly account for the properties of the actin cortex. We present a method, the Elasticity Spectra, to calculate the apparent stiffness of the cell as a function of the indentation depth and we suggest a simple phenomenological approach to measure the thickness and stiffness of the actin cortex, in addition to the standard Young's modulus. Conclusions: The Elasticity Spectra approach is tested and validated on a set of cells treated with cytoskeleton-affecting drugs, showing the potential to extend the current representation of cell mechanics, without introducing a detailed and complex description of the intracellular structure.

show abstract

Biomechanics of cultured hepatic cells during different steatogenic hits

Cited by 23 publications

References 38 publications

Elasticity Spectra as a Tool to Investigate Actin Cortex Mechanics

Elasticity Spectra as a Tool to Investigate Actin Cortex Mechanics

The use of nanovibration to discover specific and potent bioactive metabolites that stimulate osteogenic differentiation in mesenchymal stem cells

Elasticity Spectra as a Tool to Investigate Actin Cortex Mechanics

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