Biomechanical Cell Modelling Under Impact Loading

Abolfathi, N.; Karami, G.; Ziejewski, Mariusz

doi:10.1080/02286203.2008.11442500

Cited by 9 publications

(5 citation statements)

References 35 publications

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“…In this study, the volumes of the cell and nucleus are ~3000 μ m 3 and 104.5 μ m 3 , respectively, which are based on the study of McGarry et al [16]. The thickness of cell membrane is 6 nm [20, 21], and the endothelial cell membrane thickness also varies between 0.1 and 0.5 μ m [17]. The cell height is ~2–20 μ m [16, 21–23].…”

Section: Methodsmentioning

confidence: 99%

Effects of Frequency and Acceleration Amplitude on Osteoblast Mechanical Vibration Responses: A Finite Element Study

Wang

Hsu

et al. 2016

BioMed Research International

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Bone cells are deformed according to mechanical stimulation they receive and their mechanical characteristics. However, how osteoblasts are affected by mechanical vibration frequency and acceleration amplitude remains unclear. By developing 3D osteoblast finite element (FE) models, this study investigated the effect of cell shapes on vibration characteristics and effect of acceleration (vibration intensity) on vibrational responses of cultured osteoblasts. Firstly, the developed FE models predicted natural frequencies of osteoblasts within 6.85–48.69 Hz. Then, three different levels of acceleration of base excitation were selected (0.5, 1, and 2 g) to simulate vibrational responses, and acceleration of base excitation was found to have no influence on natural frequencies of osteoblasts. However, vibration response values of displacement, stress, and strain increased with the increase of acceleration. Finally, stress and stress distributions of osteoblast models under 0.5 g acceleration in Z-direction were investigated further. It was revealed that resonance frequencies can be a monotonic function of cell height or bottom area when cell volumes and material properties were assumed as constants. These findings will be useful in understanding how forces are transferred and influence osteoblast mechanical responses during vibrations and in providing guidance for cell culture and external vibration loading in experimental and clinical osteogenesis studies.

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Section: Methodsmentioning

confidence: 99%

Effects of Frequency and Acceleration Amplitude on Osteoblast Mechanical Vibration Responses: A Finite Element Study

Wang

Hsu

et al. 2016

BioMed Research International

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“…The membrane and the cytoplasm are modeled as a spherical frustum. The membrane thickness is considered as 6 nm [8]. The volume of the cell is kept constant as 3, 000 µm 3 [2].…”

Section: Modeling and Simulationmentioning

confidence: 99%

Dynamics of osteoblasts during bone remodeling cycle

Chandrashekara¹,

Chaudhary²,

Shivcharan³

et al. 2019

Vib. proced.

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Bone is a dynamic connective tissue which adjusts to load variations through continuous bone remodeling, which occurs due to the dynamic behavior of bone cells. Many researchers made attempts in obtaining the dynamic characteristics of osteoblasts and its role in bone remodeling cycle. While making an effort to understand the effects of mechanical stimuli on the osteoblast, certain ambiguity is observed in the past literatures. This paper is to demonstrate the dynamics of osteoblast cells and exhibition of different natural frequencies during its life cycle. Osteoblast is modeled as a frustum of a sphere, considering it as a continuum model. The three prominent parts of an osteoblast, i.e., membrane, cytoplasm and nucleus are considered with reported material properties. Lifespan of an active osteoblast during bone remodeling cycle is considered as 90 days and progressive osteoblast stages are analysed using Ansys. First ten natural frequencies and mode shapes are extracted for nine stages and reported. It is observed that the natural frequencies of a micron sized osteoblast are in the range of kHz. A mathematical relation for the lifespan of an active osteoblast is obtained using curve fitting for fundamental natural frequencies. The natural frequency for exciting an active osteoblast on each particular day during its lifespan can be derived from the relation. This relation can serve as a guiding tool in bioengineering for in vitro bone cell culturing. Results also throw light on the excitation frequency and natural frequency of an osteoblast for proper analysis purpose. The different modes of vibration of osteoblast is identified and reported.

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“…cell membrane, cytoplasm and nucleus. In this study, the whole volume of the cell is about 3000μm3, the volume of the nucleus is ~105μm3, the membrane thickness is ~ 6 nm [6][7][8] and the cell height is ~ 13μm. A shell element S4R and a solid element C3D8R were used to represent cell membrane and cytoplasm/nucleus, respectively.…”

Section: Geometrymentioning

confidence: 99%