Biomechanical and Microstructural Characteristics of a Collagen Film-Based Corneal Stroma Equivalent

Crabb, Rachael A.B.; Chau, Eric; Evans, M.C.W.; Barocas, Victor H.; Hubel, Allison

doi:10.1089/ten.2006.12.ft-119

Cited by 6 publications

(6 citation statements)

References 42 publications

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“…This has motivated several attempts to develop tissue engineered corneal replacements. [3][4][5] The cornea has three main layers-the epithelium, endothelium, and stroma. 1 The stroma contains quiescent cells called keratocytes that reside within uniformly aligned collagen fibrils, 30-35 nm in diameter (see Supporting Information).…”

Section: Introductionmentioning

confidence: 99%

Collagen fibril diameter and alignment promote the quiescent keratocyte phenotype

Muthusubramaniam

Peng

Zaitseva

et al. 2011

J Biomedical Materials Res

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In this study, we investigated how matrix nanotopography affects corneal fibroblast phenotype and matrix synthesis. To this end, corneal fibroblasts isolated from bovine corneas were grown on collagen nanofiber scaffolds of different diameters and alignment – 30 nm aligned fibrils (30A), 300 nm or larger aligned fibrils (300A), and 30 nm nonaligned fibrils (30NA) in comparison to collagen coated flat glass substrates (FC). Cell morphology was visualized using confocal microscopy. Quantitative PCR was used to measure expression levels of six target genes: the corneal crystallin - transketolase (TKT), the myofibroblast marker - α-smooth muscle actin (SMA), and four matrix proteins - collagen 1 (COL1), collagen 3 (COL3), fibronectin (FN) and biglycan. It was found that SMA expression was down-regulated and TKT expression was increased on all three collagen nanofiber substrates, compared to the FC control substrates. However, COL3 and biglycan expression was also significantly increased on 300A, compared to the FC substrates. Thus matrix nanotopography down-regulates the fibrotic phenotype, promotes formation of the quiescent keratocyte phenotype and influences matrix synthesis. These results have significant implications for the engineering of corneal replacements and for promoting regenerative healing of the cornea after disease and/or injury.

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Section: Introductionmentioning

confidence: 99%

Collagen fibril diameter and alignment promote the quiescent keratocyte phenotype

Muthusubramaniam

Peng

Zaitseva

et al. 2011

J Biomedical Materials Res

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“…In these cultures, the cell-scaffold interactions resulted in changes in the mechanical and permeability properties of the gels. 19,20 These results showed that stromal cells were able to modulate their own environments, by remodelling the matrix support and changing the properties more to that of a natural stroma. In fact, the resultant tissue had a lamellar-like microstructure following 21 days of incubation compared to its initial spongy structure.…”

Section: Acellular Tissue-engineered Scaffolds To Promote Endogenous mentioning

confidence: 90%

Artificial corneas: a regenerative medicine approach

Griffith

Jackson

Lagali

et al. 2009

Eye

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“…fibres and matrix. Parameter E can be used to describe the combined response of the corneal fibres and matrix (Dunn andSilver 1983, Edmund 1988), whereas when appropriately transformed, and τi can describe corneal matrix behaviour (Dunn and Silver 1983, Crabb et al 2006, Zhou et al 2011.…”

Section: Methodsmentioning

confidence: 99%

A hybrid approach to determining cornea mechanical properties in vivo using a combination of nano-indentation and inverse finite element analysis

Abyaneh

Wildman

Ashcroft

et al. 2013

Journal of the Mechanical Behavior of Biomedical Materials

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A hybrid approach to determining cornea mechanical properties in vivo using a combination of nano-indentation and inverse finite element analysis approach. A validation experiment using nano-indentation showed that an isotropic GM model was insufficient for describing the corneal material behaviour when exposed to a complex stress state. A new technique was proposed for determining the properties, using a combination of nano-indentation experiment, an isotropic and orthotropic GM model and inverse finite element method. The good agreement using this method suggests that this is a promising technique for measuring material properties in vivo and further work should focus on the reliability of the approach in

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Biomechanical and Microstructural Characteristics of a Collagen Film-Based Corneal Stroma Equivalent

Cited by 6 publications

References 42 publications

Collagen fibril diameter and alignment promote the quiescent keratocyte phenotype

Collagen fibril diameter and alignment promote the quiescent keratocyte phenotype

Artificial corneas: a regenerative medicine approach

A hybrid approach to determining cornea mechanical properties in vivo using a combination of nano-indentation and inverse finite element analysis

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