Biomechanical and Histologic Observations of Vocal Fold Fibrous Proteins

Gray, Steven D.; Alipour, Fariborz; Titze, Ingo R.; Hammond, Thomas H.

doi:10.1177/000348940010900115

Cited by 222 publications

(266 citation statements)

References 19 publications

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“…Interestingly, the trends in collagen and elastin production with respect to alterations in initial scaffold modulus/mesh size appear to mimic those observed in native LP [55][56][57]. Specifically, the SLP is a relatively loose, low modulus, connective tissue, containing low levels of collagen and elastin relative to deeper regions of the LP [55][56][57].…”

Section: Discussionmentioning

confidence: 70%

“…Specifically, the SLP is a relatively loose, low modulus, connective tissue, containing low levels of collagen and elastin relative to deeper regions of the LP [55][56][57]. The ILP and DLP are traditionally demarcated from the SLP by a marked increase in tissue density, stiffness, and elastin and collagen levels [55][56][57]. Thus, the initial 10% 10 kDa hydrogel material properties and stimulated ECM production correspond to those of the SLP, whereas the initial 20% 10 kDa and 30% 10 kDa hydrogel material properties and resultant ECM synthesis are more similar to those of the ILP and DLP.…”

Section: Discussionmentioning

confidence: 99%

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Influence of hydrogel mechanical properties and mesh size on vocal fold fibroblast extracellular matrix production and phenotype

et al. 2008

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Current clinical management of vocal fold (VF) scarring produces inconsistent and often suboptimal results. Researchers are investigating a number of alternative treatments for VF lamina propria (LP) scarring, including designer implant materials for functional LP regeneration. In the present study, we investigate the effects of the initial scaffold elastic modulus and mesh size on encapsulated VF fibroblast (VFF) extracellular matrix (ECM) production toward rational scaffold design. Polyethylene glycol diacrylate (PEGDA) hydrogels were selected for this study since their material properties, including mechanical properties, mesh size, degradation rate and bioactivity, can be tightly controlled and systematically modified. Porcine VFF were encapsulated in four PEGDA hydrogels with degradation half lives of ~25 days and initial elastic compressive moduli ranging from ~30 to 100 kPa and initial mesh sizes ranging from ~9 to 27 nm. After 30 days of static culture, VFF ECM production and phenotype in each formulation was assessed biochemically and histologically. Sulfated glycosaminoglycan synthesis increased in similar degree with both increasing initial modulus and decreasing initial mesh size. In contrast, elastin production decreased with increasing initial modulus but increased with decreasing initial mesh size. Both collagen deposition and the induction of a myofibroblastic phenotype depended strongly on initial mesh size but appeared largely unaffected by variations in initial modulus. The present results indicate that scaffold mesh size warrants further investigation as a critical regulator of VFF ECM synthesis. Furthermore, this study validates a systematic and controlled approach for analyzing VFF response to scaffold properties, which should aid in rational scaffold selection/design.

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Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Influence of hydrogel mechanical properties and mesh size on vocal fold fibroblast extracellular matrix production and phenotype

et al. 2008

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“…Tissue engineering research uses regenerative protocols that involve the engineered recapitulation of certain embryonic events [21] that coordinate the morphogenesis of the targeted tissue. The extracellular matrix (ECM) is recognized as a highly hydrated network which comprises several main effectors [22][23][24]. Its architecture provides not only an instructive cue for the conversion and spatial orientation [25] of precursor cells into a functional tissue [26] but also properties at a macroscale level provide a number of tissue functions including osmosis, molecular and nutrient transport [12,27].…”

Section: Discussionmentioning

confidence: 99%

Human clinical experience with adipose precursor cells seeded on hyaluronic acid-based spongy scaffolds

et al. 2008

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a b s t r a c tHistioconductive approaches to soft-tissue defects use scaffolds seeded with lineage-and tissue-specific progenitors to generate tissue which should reside in equilibrium with adjacent tissue. Scaffolds guide histiogenesis by ensuring cell-cell and cell-matrix interactions. Hyaluronic acid-based (HA) preadipocyte-seeded scaffolds were evaluated for their adipo-conductive potential and efficacy in humans. Preadipocytes were isolated from lipoaspirate material and seeded on HA scaffolds. The cellular biohybrid (ADIPOGRAFT Ò ) and an acellular control scaffold (HYAFF Ò 11) were implanted subcutaneously. At specific time points (2, 8 and 16 weeks) explants were analyzed histopathologically with immunohistochemistry. No adverse tissue effects occurred. Volume loss and consistent degradation of the HYAFF Ò 11 scaffolds compared to the ADIPOGRAFT Ò group indicated progressive tissue integration. No consistent histological differences between both groups were observed. By 8 weeks all void spaces within the scaffolds were filled with cells with pronounced matrix deposition in the ADIPOGRAFT Ò bio-hybrids. Here we show that HA scaffolds were stable cell carriers and had the potential to generate volumeretaining tissue. However, no adipogenic differentiation was observed within the preadipocyte-seeded scaffolds.

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“…En la LP adulta se distinguen tres tipos diferentes de elastina: oxytalan y elanium (elastina inmadura y más escasa) y elastina madura (fibras elásti-cas). Las primeras se concentran principalmente en la capa superficial de la LP, y la elastina madura, más abundante y tupida, en las capas intermedia y profunda de la LP (Gray, Alipour, Titze y Hammond, 2000;Moore and Thibeault, 2012;Thibeault, Gray, Bless, Chan y Ford, 2002).…”

Section: Evidencias Vocales Del Déficit De Elastinaunclassified

What voice tells us about genetic syndromes: The case of Williams syndrome

Guía

Heinze

Gómez-Vilda

2017

loquens

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Citation / Cómo citar este artículo: Hidalgo de la Guía, I., Garayzábal Heinze, E. y Gómez Vilda, P. (2017). Lo que la voz nos cuenta acerca de los síndromes genéticos: El caso del síndrome de Williams. Loquens, 4(1), e039. doi: http://dx.doi. org/10.3989/loquens.2017.039 RESUMEN: En el ámbito de las enfermedades raras de origen genético, la observación de la voz es una herramienta muy ventajosa para detectar diferentes condiciones clínicas asociadas a una determinada patología minoritaria. En el caso del síndrome de Williams (SW), considerada minoritaria, el estudio de la biomecánica laríngea ha demostrado que, en efecto, las diversas alteraciones clínicas asociadas a este síndrome pueden ser identificadas a través del estudio de la voz. Para llevar a cabo la investigación se analizaron 60 muestras de voz de 12 niños con síndrome de Williams y se confrontaron con 483 voces normativas procedentes de 97 niños sanos. Para el análisis acústico y biomecánico de las fonaciones se utilizó BioMetroPhon (Gómez et al., 2013). Los resultados evidencian la relación estrecha existente entre el cuadro clínico de una persona y su fonación. Este estudio es otra muestra más de la interdisciplinariedad del abordaje de la voz y de lo provechoso de su observación.Palabras clave: síndrome de Williams; déficit de elastina; voz; biomecánica laríngea; cubierta del pliegue vocal; temblor laríngeo. ABSTRACT: What voice tells us about genetic syndromes:The case of Williams syndrome. -The study of the voice is a very valuable tool in the field of rare diseases. That is because the biomechanical and acoustic analysis of phonation allows to detect specific features of a particular genetic and rare syndrome. In the case of Williams syndrome (WS), it was proved that some clinical features connected to this syndrome can be observed by means of voice analysis. In order to carry out this study, 60 recordings of 12 children suffering from WS were assessed and compared to 483 normative phonations of 97 children with typical development. The methodology used to assess the voices was BioMetroPhon (Gómez et al., 2013). The results may aim to establish a narrow connection between WS phonation and its typical genotype and, in consequence, the results of this study may highlight the interdisciplinary nature of the assessment of the voice.Keywords: Williams syndrome; elastin deficit; voice; larynx biomechanics; vocal fold cover; tremor.Copyright: © 2017 CSIC. Este es un artículo de acceso abierto distribuido bajo los términos de una licencia de uso y distribución Creative Commons Attribution (CC-by) España 3.0. INTRODUCCIÓNEl estudio de la voz actualmente ha pasado de ser considerado como un campo específicamente relacionado con un grupo reducido de profesionales (locutores, cantantes, actores, etcétera), a extender su ámbito de aplicación al conjunto de la población, puesto que, por una u otra circunstancia, la diversidad de la información que proporciona permite abordar diferentes fenómenos en el estudio del comportamiento humano. A través de su aná-li...

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Biomechanical and Histologic Observations of Vocal Fold Fibrous Proteins

Cited by 222 publications

References 19 publications

Influence of hydrogel mechanical properties and mesh size on vocal fold fibroblast extracellular matrix production and phenotype

Influence of hydrogel mechanical properties and mesh size on vocal fold fibroblast extracellular matrix production and phenotype

Human clinical experience with adipose precursor cells seeded on hyaluronic acid-based spongy scaffolds

What voice tells us about genetic syndromes: The case of Williams syndrome

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