Biomechanical and functional properties of trophoblast cells exposed to Group B Streptococcus in vitro and the beneficial effects of uvaol treatment

Botelho, Rayane Martins; Tenório, Luciana Lucena; Silva, A.L.M.; Tanabe, Eloiza; Pires, Keyla; Gonçalves, Camilla M.; Santos, Jademilson Celestino dos; Marques, Aldilane Lays Xavier; Allard, Marie-Julie; Bergeron, Julie; Sébire, Guillaume; Silva, E.C.O.; Souza, Samuel T.; Fonseca, Eduardo J.S.; Borbely, Alexandre Urban; Borbely, Karen Steponavicius Cruz

doi:10.1016/j.bbagen.2019.06.012

Cited by 11 publications

(18 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This result confirms previous studies that demonstrated the absence of toxicity of uvaol to distinct cell types [24]. Of note, uvaol prevents cell death caused by bacterial products [25], and has protector effects against heavy metal-induced cytotoxicity [26].…”

Section: Discussionsupporting

confidence: 92%

Uvaol Improves the Functioning of Fibroblasts and Endothelial Cells and Accelerates the Healing of Cutaneous Wounds in Mice

et al. 2020

View full text Add to dashboard Cite

Uvaol is a natural pentacyclic triterpene that is widely found in olives and virgin olive oil, exerting various pharmacological properties. However, information remains limited about how it affects fibroblasts and endothelial cells in events associated with wound healing. Here, we report the effect of uvaol in the in vitro and in vivo healing process. We show the positive effects of uvaol on migration of fibroblasts and endothelial cells in the scratch assay. Protein synthesis of fibronectin and laminin (but not collagen type I) was improved in uvaol-treated fibroblasts. In comparison, tube formation by endothelial cells was enhanced after uvaol treatment. Mechanistically, the effects of uvaol on cell migration involved the PKA and p38-MAPK signaling pathway in endothelial cells but not in fibroblasts. Thus, the uvaol-induced migratory response was dependent on the PKA pathway. Finally, topical treatment with uvaol caused wounds to close faster than in the control treatment using experimental cutaneous wounds model in mice. In conclusion, uvaol positively affects the behavior of fibroblasts and endothelial cells, potentially promoting cutaneous healing.

show abstract

Section: Discussionsupporting

confidence: 92%

Uvaol Improves the Functioning of Fibroblasts and Endothelial Cells and Accelerates the Healing of Cutaneous Wounds in Mice

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Our study supports previous findings that suggest that the trophoblast may respond differently to different bacteria [46][47][48][49][50][51][52][53]. The results support the existence of separate mechanisms of bacterial recognition by trophoblasts, or the presence of bacterial products leading to alternative inflammatory/anti-inflammatory responses.…”

Section: Resultssupporting

confidence: 91%

“…The degree to which trophoblasts participate in the control of potential placental-colonizing microbiota requires further study. Furthermore, previous studies have suggested that trophoblasts may respond differently to different bacteria [46][47][48][49][50][51][52][53]. In this paper, we compare the cytokine patterns exhibited by Swan 71 (Sw.71), immortalized human first trimester trophoblast cells [54], after in vitro interactions with E. coli (a common urogenital pathogen), L. jensenii (a vaginal commensal), and L. crispatus (a hypothesized mutualist and sentinel of the human vaginal ecosystem) [55,56].…”

Section: Introductionmentioning

confidence: 99%

Differential Secretion of Inflammatory Cytokines by Human Trophoblasts in the Presence of Escherichia coli, Lactobacillus crispatus, and Lactobacillus jensenii

Alhousseini

Vadillo-Ortega

Palacios‐González

et al. 2020

Gynecol Obstet Invest

View full text Add to dashboard Cite

Introduction: The existence of a placental microbiome would require a non-antagonistic relationship between potentially colonizing bacteria and trophoblasts. Objective: The immunologic response of trophoblasts to specific potentially invading bacteria needs further analysis. Methodology: Immortalized first trimester human trophoblasts Swan 71 (Sw.71) were coincubated with Escherichia coli, Lactobacillus jensenii, Lactobacillus crispatus, and incubated alone (i.e., control group; 4 conditions with n = 6 for each condition). Chemokines and cytokines were measured. ANOVA with post hoc pairwise analysis was used to compare cytokines/chemokines concentrations in the 4 culture media. Results: Sw.71 co-incubated with E. coli, L. jensenii or L. crispatus resulted in differential secretion of 11 of the 26 assayed cytokines/chemokines. Sw.71 co-incubated with any of the 3 bacteria responded with significant increased secretion of interleukin (IL)-8 and granulocyte macrophage colony-stimulating factor. All bacteria elicited the secretion of IL-6 and interferon (IFN) α2, 2 proinflammatory cytokines. In addition, Lactobacillus species resulted in increased secretion of IL-12p40 and IFNγ. While E. coli did not modify secretion of anti-inflammatory cytokines, Sw.71 cells responded to co-incubation with Lactobacillus species by secreting increased levels of IL-10 and IL-1ra. Both Lactobacillus species led to a decreased secretion of IL-4. Conclusion: All 3 bacterial species triggered significant release of chemokines and inflammatory cytokines, suggesting that a commensal relationship with trophoblasts may not be feasible.

show abstract

“…On the other hand, nitrite production was not observed in BeWo cells. It is possible to speculate that nitrite is harmful for immune tolerance in placental environment (villous trophoblast), however previous study showed active role of nitrite in HTR-8/SVneo cells (extravillous trophoblast) 79 , once again evidencing that differences of inflammatory mediators between trophoblasts cells are normal and expected.…”

Section: Discussionmentioning

confidence: 91%

Cyclooxygenase (COX)-2 modulates Toxoplasma gondii infection, immune response and lipid droplets formation in human trophoblast cells and villous explants

Souza

Silva

Milián

et al. 2021

Sci Rep

View full text Add to dashboard Cite

Congenital toxoplasmosis is represented by the transplacental passage of Toxoplasma gondii from the mother to the fetus. Our studies demonstrated that T. gondii developed mechanisms to evade of the host immune response, such as cyclooxygenase (COX)-2 and prostaglandin E2 (PGE2) induction, and these mediators can be produced/stored in lipid droplets (LDs). The aim of this study was to evaluate the role of COX-2 and LDs during T. gondii infection in human trophoblast cells and villous explants. Our data demonstrated that COX-2 inhibitors decreased T. gondii replication in trophoblast cells and villous. In BeWo cells, the COX-2 inhibitors induced an increase of pro-inflammatory cytokines (IL-6 and MIF), and a decrease in anti-inflammatory cytokines (IL-4 and IL-10). In HTR-8/SVneo cells, the COX-2 inhibitors induced an increase of IL-6 and nitrite and decreased IL-4 and TGF-β1. In villous explants, the COX-2 inhibitors increased MIF and decreased TNF-α and IL-10. Furthermore, T. gondii induced an increase in LDs in BeWo and HTR-8/SVneo, but COX-2 inhibitors reduced LDs in both cells type. We highlighted that COX-2 is a key factor to T. gondii proliferation in human trophoblast cells, since its inhibition induced a pro-inflammatory response capable of controlling parasitism and leading to a decrease in the availability of LDs, which are essentials for parasite growth.

show abstract

Biomechanical and functional properties of trophoblast cells exposed to Group B Streptococcus in vitro and the beneficial effects of uvaol treatment

Cited by 11 publications

References 69 publications

Uvaol Improves the Functioning of Fibroblasts and Endothelial Cells and Accelerates the Healing of Cutaneous Wounds in Mice

Uvaol Improves the Functioning of Fibroblasts and Endothelial Cells and Accelerates the Healing of Cutaneous Wounds in Mice

Differential Secretion of Inflammatory Cytokines by Human Trophoblasts in the Presence of <b><i>Escherichia coli</i></b>, <b><i>Lactobacillus crispatus</i></b>, and <b><i>Lactobacillus jensenii</i></b>

Cyclooxygenase (COX)-2 modulates Toxoplasma gondii infection, immune response and lipid droplets formation in human trophoblast cells and villous explants

Contact Info

Product

Resources

About