Biomechanical and cellular segmental characterization of human meniscus: building the basis for Tissue Engineering therapies

Pereira, Hélder; Caridade, Sofia G.; Frias, A. M.; Silva‐Correia, Joana; Cengiz, Ibrahim Fatih; Mano, João F.; Oliveira, Joaquím M.; Espregueira-Mendes, João; Reis, Rui L.

doi:10.1016/j.joca.2014.07.001

Cited by 85 publications

(66 citation statements)

References 53 publications

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“…Generally, avascular regions were observed to be at a distance of > 5 mm from the outer periphery. The difference in 2D cellularity between the vascular and avascular regions was reported in a previous study (Pereira et al , ). In the present study, the 3D cellular densities of the vascular and avascular regions were quantified to be 27 199 and 12 820 cells/mm 3 , respectively.…”

Section: Discussionsupporting

confidence: 68%

“…For this reason, some cells that are actually of different cell type could be identified to be from the same cell population, due to their similar morphology; for example, both a superficial zone cell and a fibroblast‐like cell have a fusiform morphology. In a previous study (Pereira et al , ), it was reported that the cell populations in the meniscus were positive for CD105, CD73 and CD90 markers and negative for haematopoietic markers, such as CD45 and CD34, as well as CD31. Quantification of the cell phenotypes and their individual densities were not within the aims of the present study, and should be a subject of further investigation using immunohistochemistry.…”

Section: Discussionmentioning

confidence: 89%

“…In the present study, the 3D cellular densities of the vascular and avascular regions were quantified to be 27 199 and 12 820 cells/mm 3 , respectively. A finding from a previous study (Pereira et al , ) was that the anterior segment of the meniscus possesses significantly higher damping properties than the other segments; however, on the other hand, the anterior segment has inferior 2D cellularity as compared to other segments (Pereira et al , ). This study showed that the 3D cellular density of the anterior segment was relatively higher than that of other segments.…”

Section: Discussionmentioning

confidence: 94%

“…The meniscus is a highly complex tissue (Pereira et al , ) and its regeneration is a multi‐parametric process. Fundamental studies have been deepening knowledge of the biology of the human meniscus tissue that is crucial for future advancements in regenerative strategies.…”

Section: Introductionmentioning

confidence: 99%

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Segmental and regional quantification of 3D cellular density of human meniscus from osteoarthritic knee

Cengiz

Pereira

Pêgo

et al. 2015

J Tissue Eng Regen Med

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The knee menisci have important roles in the knee joint. Complete healing of the meniscus remains a challenge in the clinics. Cellularity is one of the most important biological parameters that must be taken into account in regenerative strategies. However, knowledge on the 3D cellularity of the human meniscus is lacking in the literature. The aim of this study was to quantify the 3D cellular density of human meniscus from the osteoarthritic knee in a segmental and regional manner with respect to laterality. Human lateral menisci were histologically processed and stained with Giemsa for histomorphometric analysis. The cells were counted in an in-depth fashion. 3D cellular density in the vascular region (27 199 cells/mm ) was significantly higher than in the avascular region (12 820 cells/mm ). The cells were observed to possess two distinct morphologies, roundish or flattened. The 3D density of cells with fibrochondrocyte morphology (14 705 cells/mm ) was significantly greater than the 3D density of the cells with fibroblast-like cell morphology (5539 cells/mm ). The best-fit equation for prediction of the 3D density of cells with fibrochondrocyte morphology was found to be: Density of cells with fibrochondrocyte morphology = 1.22 × density of cells withfibroblast-like cell morphology + 7750. The present study revealed the segmental and regional 3D cellular density of human lateral meniscus from osteoarthritic knee with respect to laterality. This crucial but so far missing information will empower cellular strategies aiming at meniscus tissue regeneration. Copyright © 2015 John Wiley & Sons, Ltd.

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Section: Discussionsupporting

confidence: 68%

Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

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Segmental and regional quantification of 3D cellular density of human meniscus from osteoarthritic knee

Cengiz

Pereira

Pêgo

et al. 2015

J Tissue Eng Regen Med

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“…Low fibrillar stiffness, high permeability, and reasonably high nonfibrillar matrix modulus help tissue deformations and dissipation under prolonged loading conditions. It may be that the meniscus, which was not considered here, may be the elastic shock absorber in the knee while the tibial plateau cartilage contributes primarily to the viscous dissipation of loads (Danso et al, 2015;Gaugler et al, 2014;Pereira et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Very early osteoarthritis changes sensitively fluid flow properties of articular cartilage

Mäkelä

Han

Herzog

et al. 2015

Journal of Biomechanics

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In this study, fibril-reinforced poroelastic (FRPE) modeling was used for rabbit knee after anterior cruciate ligament transection (ACLT) to assess how the mechanical properties of collagen, proteoglycans, and fluid in articular cartilage change in early osteoarthritis, and how site-specific these changes are. Unilateral ACLT was performed in eight skeletally mature, female New Zealand white rabbits. A separate control (CTRL) group consisted of knee joints of five non-operated rabbits. Animals were sacrificed at four weeks after ACLT and cartilage-on-bone samples from femoral groove, medial and lateral femoral condyles, and tibial plateaus were harvested. A stress-relaxation protocol in indentation geometry was applied and the FRPE model was fitted to the experimental force-time curve by minimizing the mean absolute error between experiment and simulation. The optimized parameters were: fibril network modulus (Ef), representing the collagen network; non-fibrillar matrix modulus (Enf), representing the PG matrix; and permeability (k), representing fluid flow. Permeability was increased significantly in the ACLT group compared to the CTRL group knees at all sites except for the medial tibial plateau. ACLT also caused a decrease in the Ef at all sites except for the medial and lateral tibial plateaus. The Enf of the ACLT group knees was altered only for the lateral femoral condyle. The results of this study suggest that early osteoarthritis primarily affects cartilage permeability and impairs the collagen network stiffness in a site-specific manner. These findings from early osteoarthritis indicate that fluid flow velocity in articular cartilage may change prior to quantifiable structural alterations in the tissue.

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General Characterization of Physical Properties of Natural‐Based Biomaterials

Alatorre-Meda

Mano²

2016

Biomaterials From Nature for Advanced Devices and Therapies

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Biomechanical and cellular segmental characterization of human meniscus: building the basis for Tissue Engineering therapies

Abstract: Menisci are not uniform structures. Anterior segments have lower cellularity and higher damping. Cyclic loads influence viscoelastic characteristics. Future TE therapies should consider segmental architecture, cellularity and biomechanics of fresh tissue.

Cited by 85 publications

References 53 publications

Segmental and regional quantification of 3D cellular density of human meniscus from osteoarthritic knee

Segmental and regional quantification of 3D cellular density of human meniscus from osteoarthritic knee

Very early osteoarthritis changes sensitively fluid flow properties of articular cartilage

General Characterization of Physical Properties of Natural‐Based Biomaterials

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