Biomechanical and biochemical characterization of porcine tracheal cartilage

Hoffman, Benjamin S.; Martin, Matthew J.; Brown, Bryan N.; Bonassar, Lawrence J.; Cheetham, Jonathan

doi:10.1002/lary.25861

Cited by 28 publications

(31 citation statements)

References 55 publications

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“…This agrees with literature that found no significant difference in longitudinal versus circumferential biomechanical properties in a seeded scaffold with respect to suture retention [105]. Other studies have found out that cultured tissue to be anisotropic, showing a higher elastic moduli longitudinally [99], [106].…”

Section: Effect Of Growth Factor Supplementationsupporting

confidence: 92%

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Scaffold-free tracheal cartilage engineering using roller bottle culture

Sheikh¹

2021

Preprint

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Resection with primary anastomosis can only repair up to 50% of the adult trachea and up to 30% of the pediatric trachea when damaged. There is a strong clinical need for long-segment tracheal replacements. The goal of this research was to create a seamless, scaffold-free cartilage cylinder for tracheal tissue engineering in vitro. Primary bovine articular chondrocytes were seeded onto tracheal moulds for roller bottle culture and the effect of rotational speed, growth factor supplementation, and chondrocyte layering were investigated. After the 4-week culture period, samples were evaluated biochemically, histologically, and biomechanically. The results indicated that rotation was necessary for full tissue coverage, with slower rotational speeds generating thicker tissue with an improved extracellular matrix, IGF-1 supplementation generating thicker tissue rich in glycosaminoglycans with inferior mechanical properties, and chondrocyte layering producing thinner tissue with increased mechanical properties. Overall, scaffold-free tissue engineering can generate seamless cylindrical cartilage constructs using roller bottle culture for future applications in long-segment tracheal replacement.

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Section: Effect Of Growth Factor Supplementationsupporting

confidence: 92%

“…MPa) [99]. The ultimate tensile strength observed in samples with IGF-1 supplementation (1.3 MPa) were consistent with that of other scaffold-free methods (1.1…”

Section: Effect Of Growth Factor Supplementationsupporting

confidence: 87%

Scaffold-free tracheal cartilage engineering using roller bottle culture

Sheikh¹

2021

Preprint

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“…Some researchers have studied and comprehensively described the mechanical and biochemical characteristics of pig trachea, and pointed out that pig trachea is not only similar in shape to human trachea, 9 but also an excellent xenogeneic tracheal graft, which is one of the sources of tissue engineering trachea reconstruction. 10 In addition, pre-clinical studies conducted on pigs showed that there is no difference in the mechanical properties of the decellularized scaffolds before and after implantation. 11 Therefore, it is of great significance to reduce the immunogenicity of trachea while retaining the biomechanical properties for xenotracheal transplantation.…”

Section: Discussionmentioning

confidence: 99%

Biomechanical changes of freezer-storaged and decellularized pig tracheal scaffoldings

et al. 2021

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Background As an excellent xenotransplant, the pig trachea can be decellularized and cryopreserved to reduce its immunogenicity. However, few reports are found on the changes of its mechanical properties after cryopreservation and decellularization. Objective To evaluate the structure and biomechanical properties in pig tracheal scaffolds resulting from decellularized and cryopreserved. Material and methods Twenty-five pig tracheal segments were separated into five groups: untreated (group A), only decellularized (group B), only cryopreserved (group C), decellularized after cryopreserved (group D) and cryopreserved after decellularized (group E). Tracheal segments were subjected to uniaxial tension or compression using a universal testing machine to determine structural biomechanical changes. Results It showed that there was no statistically significant difference in the tensile strength of the trachea in each group. The compressive strength of group B, C and D were same as the group A ( P > 0.05), while the group E was lower than that of the group A ( P < 0.05). Conclusions and significance: The histological examination of the decellularization after cryopreservation shows that the removal of epithelial cells and submucosal cells is more thorough, and the biomechanical structure of the trachea is better preserved. This proved to be a new method to prepare xenotransplantation of trachea graft.

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“…In humans, the mechanical properties of the tracheal cartilage are gender and age dependent, with the tensile Young´s modulus reported in the range of 1-25 MPa [36][37][38]. Taking into consideration that the porcine trachea model was generally believed to resemble more closely that of human, the compression modulus of the porcine's trachea has been reported in the range of 1-5 MPa [39,40]. It is of note that the tensile modulus of the porous 3D-TIPS scaffolds with 50% infill density (∼1 MPa) is close to the low end of these tissue values and the decellularized rat trachea scaffolds (∼939 kPa), but much higher than collagen hydrogel (1-10 kPa) [41][42][43].…”

Section: Discussionmentioning

confidence: 99%

Human airway-like multilayered tissue on 3D-TIPS printed thermoresponsive elastomer/collagen hybrid scaffolds

Magaz

Huo

et al. 2020

Acta Biomaterialia

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Developing a biologically representative complex tissue of the respiratory airway is challenging, however, beneficial for treatment of respiratory diseases, a common medical condition representing a leading cause of death in the world. This study reports a successful development of synthetic human tracheobronchial epithelium based on interpenetrated hierarchical networks composed of a reversely 3D printed porous structure of a thermoresponsive stiffness-softening elastomer nanohybrid impregnated with collagen nanofibrous hydrogel in vitro. Human bronchial epithelial cells (hBEpiCs) were able to attach and grow into an epithelial monolayer on the hybrid scaffolds co-cultured with either human bronchial fibroblasts (hBFs) or human bone-marrow derived mesenchymal stem cells (hBM-MSCs), with substantial enhancement of mucin expression, ciliation, well-constructed intercellular tight junctions and adherens junctions. The multi-layered co-culture 3D scaffolds consisting of a top monolayer of differentiated epithelium, with either hBFs or hBM-MSCs proliferating within the hyperelastic nanohybrid scaffold underneath, created a tissue analogue of the upper respiratory tract, validating these 3D printed guided scaffolds as a platform to support co-culture and cellular organization. In particular, hBM-MSCs in the co-culture system promoted an overall matured physiological tissue analogue of the respiratory system, a promising synthetic tissue for drug discovery, tracheal repair and reconstruction.

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Biomechanical and biochemical characterization of porcine tracheal cartilage

Abstract: NA. Laryngoscope, 126:E325-E331, 2016.

Cited by 28 publications

References 55 publications

Scaffold-free tracheal cartilage engineering using roller bottle culture

Scaffold-free tracheal cartilage engineering using roller bottle culture

Biomechanical changes of freezer-storaged and decellularized pig tracheal scaffoldings

Human airway-like multilayered tissue on 3D-TIPS printed thermoresponsive elastomer/collagen hybrid scaffolds

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