Context: Single-limb squats on a decline angle have been suggested as a rehabilitative intervention to target the knee extensors. Investigators, however, have presented very little empirical research in which they have documented the biomechanics of these exercises or have determined the optimum angle of decline used.Objective: To determine the involvement of the gastrocnemius and rectus femoris muscles and the external ankle and knee joint moments at 606 of knee flexion while performing a single-limb squat at different decline angles.Design: Participants acted as their own controls in a repeated-measures design.Patients or Other Participants: We recruited 10 participants who had no pain, injury, or neurologic disorder.Intervention(s): Participants performed single-limb squats at different decline angles.Main Outcome Measure(s): Angle-specific knee and ankle moments were calculated at 606 of knee flexion. Angle-specific electromyography (EMG) activity was calculated at 606 of knee flexion. Integrated EMG also was calculated to determine the level of muscle activity over the entire squat.Results: An increase was seen in the knee moments (P , .05) and integrated EMG in the rectus femoris (P , .001) as the decline angle increased. A decrease was seen in the ankle moments as the decline angle increased (P 5 .001), but EMG activity in the gastrocnemius increased between 166 and 246 (P 5 .018).Conclusions: As the decline angle increased, the knee extensor moment and EMG activity increased. As the decline angle increased, the ankle plantar-flexor moments decreased; however, an increase in the EMG activity was seen with the 246 decline angle compared with the 166 decline angle. This indicates that decline squats at an angle greater than 166 may not reduce passive calf tension, as was suggested previously, and may provide no mechanical advantage for the knee.Key Words: knee moments, electromyography, movement analysis Key Points N The 166 decline angle provided the maximum benefit for the knee extensors with the minimum effect for the ankle. N The 246 decline angle provided a greater challenge to the ankle and targeted the knee extensors. N The single-limb squat may produce significant cocontractions about the knee and ankle joints that the external net joint moments alone do not reflect.