Biomechanical analysis of structural deformation in living cells

Bader, Dan L.; Knight, Martin M.

doi:10.1007/s11517-008-0381-4

Cited by 37 publications

(26 citation statements)

References 81 publications

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“…This constant strain was maintained while the constructs relaxed until an equilibrium stress was reached. From the stress-strain response a tangent modulus was estimated, while an equilibrium modulus was calculated following stress relaxation [29]. …”

Section: Methodsmentioning

confidence: 99%

Biomechanical Conditioning Enhanced Matrix Synthesis in Nucleus Pulposus Cells Cultured in Agarose Constructs with TGFβ

Tilwani

Bader

Chowdhury

2012

JFB

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Biomechanical signals play an important role in normal disc metabolism and pathology. For instance, nucleus pulposus (NP) cells will regulate metabolic activities and maintain a balance between the anabolic and catabolic cascades. The former involves factors such as transforming growth factor-β (TGFβ) and mechanical stimuli, both of which are known to regulate matrix production through autocrine and paracrine mechanisms. The present study examined the combined effect of TGFβ and mechanical loading on anabolic activities in NP cells cultured in agarose constructs. Stimulation with TGFβ and dynamic compression reduced nitrite release and increased matrix synthesis and gene expression of aggrecan and collagen type II. The findings from this work has the potential for developing regenerative treatment strategies which could either slow down or stop the degenerative process and/or promote healing mechanisms in the intervertebral disc.

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Section: Methodsmentioning

confidence: 99%

Biomechanical Conditioning Enhanced Matrix Synthesis in Nucleus Pulposus Cells Cultured in Agarose Constructs with TGFβ

Tilwani

Bader

Chowdhury

2012

JFB

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“…Knowledge of the mechanics of articular cartilage may improve the understanding of mechanotransduction processes in the tissue that play important roles in the progression of joint disease [5]. Articular cartilage is anisotropic, inhomogeneous and nonlinear in nature.…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional fibril-reinforced finite element model of articular cartilage

Cheung

2009

Med Biol Eng Comput

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Collagen fiber orientations in articular cartilage are tissue depth-dependent and joint site-specific. A realistic three-dimensional (3D) fiber orientation has not been implemented in modeling fluid flow-dependent response of articular cartilage; thus the detailed mechanical role of the collagen network may have not been fully understood. In the present study, a previously developed fibril-reinforced model of articular cartilage was extended to account for the 3D fiber orientation. A numerical procedure for the material model was incorporated into the finite element code ABAQUS using the "user material" option. Unconfined compression and indentation testing was evaluated. For indentation testing, we considered a mechanical contact between a solid indenter and a medial femoral condyle, assuming fiber orientations in the surface layer to follow the split-line pattern. The numerical results from the 3D modeling for unconfined compression seemed reasonably to deviate from that of axisymmetric modeling. Significant fiber orientation dependence was observed in the displacement, fluid pressure and velocity for the cases of moderate strain-rates, or during early relaxation. The influence of fiber orientation diminished at static and instantaneous compressions.

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“…Therefore, imaging of living cells subject to various types of mechanical stimulation is becoming an indispensable tool to investigate mechanobiology and mechanotransduction on the level of single molecules (14). A multitude of devices and systems for mechanical stretching of tissues or cells ex vivo has been developed and is reviewed in detail by others (2,3).…”

mentioning

confidence: 99%

A device for simultaneous live cell imaging during uni-axial mechanical strain or compression

Gerstmair¹,

Fois²,

Innerbichler³

et al. 2009

Journal of Applied Physiology

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Mechanical stimuli control multiple cellular processes such as secretion, growth, and differentiation. A widely used method to investigate cell strain ex vivo is stretching an elastic membrane to which cells adhere. However, simultaneous imaging of dynamic signals from single living cells grown on elastic substrates during uni-axial changes of cell length is usually hampered by the movement of the sample along the strain axis out of the narrow optical field of view. We used a thin, prestrained, elastic chamber as growth substrate for the cells and deformed the chamber with a computer-controlled stretch device. An algorithm that compensates the lateral displacement during stretch kept any selected point of the whole chamber at a constant position on the microscope during strain or relaxation (compression). Adherent cells or other materials that adhere to the bottom of the chamber at any given position could be imaged during controlled positive (stretch) or negative (compression) changes of cell length. The system was tested on living alveolar type II cells, in which mechanical effects on secretion have been intensively investigated in the past.

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Biomechanical analysis of structural deformation in living cells

Cited by 37 publications

References 81 publications

Biomechanical Conditioning Enhanced Matrix Synthesis in Nucleus Pulposus Cells Cultured in Agarose Constructs with TGFβ

Biomechanical Conditioning Enhanced Matrix Synthesis in Nucleus Pulposus Cells Cultured in Agarose Constructs with TGFβ

Three-dimensional fibril-reinforced finite element model of articular cartilage

A device for simultaneous live cell imaging during uni-axial mechanical strain or compression

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