Biomaterials for Tissue Engineering

Eisenbarth, E.

doi:10.1002/adem.200700287

Cited by 69 publications

(53 citation statements)

References 99 publications

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“…An ideal scaffold should be biocompatible, biodegradable and does not induce an immune reaction or inflammation. These scaffolds can be obtained from natural or synthetic polymers [2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Extraction and Characterization of Chitin and Chitosan from Blue Crab and Synthesis of Chitosan Cryogel Scaffolds

Bölgen¹,

Demir²,

Öfkeli³

et al. 2016

J. Turk. Chem. Soc., Sect. A: Chem.

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Polymeric scaffolds produced by cryogelation technique have attracted increasing attention for tissue engineering applications. Cryogelation is a technique which enables to produce interconnected porous matrices from the frozen reaction mixtures of polymers or monomeric precursors. Chitosan is a biocompatible, biodegradable, nontoxic, antibacterial, antioxidant, and antifungal natural polymer that is obtained by deacetylation of chitin, which is mostly found in the exoskeleton of many crustaceans. In this study, chitin was chemically isolated from the exoskeleton of blue crab (Callinectes sapidus). Callinectes sapidus samples were collected from a market, as a waste material after it has been consumed as food. Demineralization, deproteinization, and decolorization steps were applied to the samples to obtain chitin. Chitosan was prepared from isolated chitin by deacetylation at high temperatures. The chemical composition of crab shell, extracted chitin and chitosan were characterized with FTIR analysis. Moreover, in order to determine the physicochemical and functional properties of the produced chitosan, solubility, water uptake, and oil uptake analysis were performed. Chitosan cryogel scaffolds were prepared by crosslinking reaction at cryogenic conditions at constant amount of chitosan (1%, w/v) with different ratios of glutaraldehyde (1, 3, and 6%, v/v) as crosslinker. The chemical structure of the scaffolds were examined by FTIR. Also, the water uptake capacity of scaffolds have been determined. Collectively, the results suggested that the characterized chitosan cryogels can be potential scaffolds to be used in tissue engineering applications.

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Section: Introductionmentioning

confidence: 99%

Extraction and Characterization of Chitin and Chitosan from Blue Crab and Synthesis of Chitosan Cryogel Scaffolds

Bölgen¹,

Demir²,

Öfkeli³

et al. 2016

J. Turk. Chem. Soc., Sect. A: Chem.

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“…More recently, Williams has defined tissue engineering as 'the creation (or formation) of new tissue for the therapeutic reconstruction of the human body, by the deliberate and controlled stimulation of selected target cells through a systematic combination of molecular and mechanical signals' [2]. Although no biomaterial is mentioned in these definitions, tissue engineering does need a biomaterial as a framework for single cells to build a vital and well-functioning tissue [3]. These scaffolds act as an extracellular matrix whose three-dimensional architecture organizes the cells, directing the growth and formation of a desired tissue [4], and facilitates the delivery of molecular and mechanical signals [5].…”

Section: Introductionmentioning

confidence: 99%

“…These different pore size domains affect such processes as vascularization, which ensures the transport of oxygen, nutrients and waste, and migration of different cell populations, which is necessary for cell survival and an adequate implant-tissue attachment [3,9,10].…”

Section: Introductionmentioning

confidence: 99%

Control of the pore architecture in three-dimensional hydroxyapatite-reinforced hydrogel scaffolds

Román

Cabañas

Peña

et al. 2011

Science and Technology of Advanced Materials

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Hydrogels (gellan or agarose) reinforced with nanocrystalline carbonated hydroxyapatite (nCHA) were prepared by the GELPOR3D technique. This simple method is characterized by compositional flexibility; it does not require expensive equipment, thermal treatment, or aggressive or toxic solvents, and yields a three-dimensional (3D) network of interconnected pores 300-900 µm in size. In addition, an interconnected porosity is generated, yielding a hierarchical porous architecture from the macro to the molecular scale. This porosity depends on both the drying/preservation technology (freeze drying or oven drying at 37• C) and on the content and microstructure of the reinforcing ceramic. For freeze-dried samples, the porosities were approximately 30, 66 and below 3% for pore sizes of 600-900 µm, 100-200 µm and 50-100 nm, respectively. The pore structure depends much on the ceramic content, so that higher contents lead to the disappearance of the characteristic honeycomb structure observed in low-ceramic scaffolds and to a lower fraction of the 100-200-µm-sized pores. The nature of the hydrogel did not affect the pore size distribution but was crucial for the behavior of the scaffolds in a hydrated medium: gellan-containing scaffolds showed a higher swelling degree owing to the presence of more hydrophilic groups.

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“…[35][36][37][38] Therefore, a scaffold intended for use in TE should facilitate the attachment, migration and growth of cells and should result finally in the formation of a functional tissue. [39] In this study, we performed indirect and direct contact-based cytotoxicity assays, where the former gives a measure of toxic effects of the scaffold on cells and the latter gives an estimate of a scaffold's ability to support cell adhesion and growth.…”

Section: Resultsmentioning

confidence: 99%

Antheraea assama Silk Fibroin‐Based Functional Scaffold with Enhanced Blood Compatibility for Tissue Engineering Applications

Kasoju

Bora

2010

Adv Eng Mater

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The architecture and surface chemistry of a scaffold determine its utility in tissue engineering (TE). Conventional techniques have limitations in fabricating a scaffold with control over both architecture and surface chemistry. To ameliorate this, in this report, we demonstrate the fabrication of an Antheraea assama silk fibroin (AASF)‐based functional scaffold. AASF is a non‐mulberry variety having superior qualities to mulberry SF and is largely unexplored in the context of TE. First, a 3D scaffold with biomimetic architecture is fabricated. The scaffold is subsequently made blood compatible by modifying the surface chemistry through a simple sulfation reaction. EDX and FTIR analysis demonstrate the successful sulfation of the scaffold. SEM observations reveal that sulfation has no any effect on the scaffold architecture. TGA reveals that it has increased thermal stability. The sulfation reaction significantly improves the overall hydrophilicity of the scaffold, as is evident from the increase in water holding capacity; this possibly enhances the blood compatibility. The enhancement in blood compatibility of the sulfated scaffold is determined from in vitro haemolysis, protein adsorption and platelet adhesion studies. The sulfated scaffold is non‐toxic and supports cell adhesion and growth, as revealed by indirect and direct contact‐based in vitro cytotoxicity assays. This study reveals that the AASF‐based functional scaffold, which has biomimetic architecture and blood‐compatible surface chemistry, could be suitable for TE applications.

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Biomaterials for Tissue Engineering

Cited by 69 publications

References 99 publications

Extraction and Characterization of Chitin and Chitosan from Blue Crab and Synthesis of Chitosan Cryogel Scaffolds

Extraction and Characterization of Chitin and Chitosan from Blue Crab and Synthesis of Chitosan Cryogel Scaffolds

Control of the pore architecture in three-dimensional hydroxyapatite-reinforced hydrogel scaffolds

Antheraea assama Silk Fibroin‐Based Functional Scaffold with Enhanced Blood Compatibility for Tissue Engineering Applications

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