Biomaterial–stem cell interactions and their impact on stem cell response

Schaap‐Oziemlak, Aneta M.; Kühn, Philipp; Kooten, Theo G. van; Rijn, Patrick van

doi:10.1039/c4ra07915a

Cited by 45 publications

(43 citation statements)

References 110 publications

(161 reference statements)

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“…Controlling the interface between biomaterials and biological entities such as proteins and cells represents the key for sophisticated cell cultures, implants, and disease models for drug screening. In the last decades, the design of biomaterials was widely investigated to direct cellular behavior . Besides the surface characteristics of the biomaterial such as its wettability, charge, chemistry, and topography, the materials mechanical stability and porosity were defined as crucial parameters for cell–matrix interactions.…”

Section: Introductionmentioning

confidence: 99%

Tyramine‐conjugated alginate hydrogels as a platform for bioactive scaffolds

Schulz

Gepp

Stracke

et al. 2018

J Biomedical Materials Res

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Alginate‐based hydrogels represent promising microenvironments for cell culture and tissue engineering, as their mechanical and porous characteristics are adjustable toward in vivo conditions. However, alginate scaffolds are bioinert and thus inhibit cellular interactions. To overcome this disadvantage, bioactive alginate surfaces were produced by conjugating tyramine molecules to high‐molecular‐weight alginates using the carbodiimide chemistry. Structural elucidation using nuclear magnetic resonance spectroscopy and contact angle measurements revealed a surface chemistry and wettability of tyramine‐alginate hydrogels similar to standard cell culture treated polystyrene. In contrast to stiff cell culture plastic, tyramine‐alginate scaffolds were found to be soft (60–80 kPa), meeting the elastic moduli of human tissues such as liver and heart. We further demonstrated an enhanced protein adsorption with increasing tyramine conjugation, stable for several weeks. Cell culture studies with human mesenchymal stem cells and human pluripotent stem cell‐derived cardiomyocytes qualified tyramine‐alginate hydrogels as bioactive platforms enabling cell adhesion and contraction on (structured) 2‐D layer and spherical matrices. Due to the alginate functionalization with tyramines, stable cell–matrix interactions were observed beneficial for an implementation in biology, biotechnology, and medicine toward efficient cell culture and tissue substitutes. © 2018 The Authors. Journal of Biomedical Materials Research Part A published by Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 114–121, 2019.

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Section: Introductionmentioning

confidence: 99%

Tyramine‐conjugated alginate hydrogels as a platform for bioactive scaffolds

Schulz

Gepp

Stracke

et al. 2018

J Biomedical Materials Res

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“…Once PHAs are implanted in vivo, it is the surface that interacts with the host (e.g., cells, bacteria, etc.). Accordingly, surface properties are extremely important as they determine the interactions at the biomaterial–biological environment …”

Section: Introductionmentioning

confidence: 99%

“…Accordingly, surface properties are extremely important as they determine the inter actions at the biomaterial-biological environment. [8,9] Surface mimicking natural extracellular matrix supports cell adhesion, proliferation, and differentiation that can be influenced by surface biochemical, biophysical, and micro environment. [10] Cells behave differently toward surfaces being modified with a variety of functional groups.…”

Section: Introductionmentioning

confidence: 99%

Surface Modification of Polyhydroxyalkanoates toward Enhancing Cell Compatibility and Antibacterial Activity

Liu

Zhang

et al. 2017

Macro Materials & Eng

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Biomaterials for in vivo application should induce positive interaction with various histocytes and inhibit bacteria inflection as well. Cells and/or bacteria response to the extracellular environment is therefore the basic principle to design the biomaterials surface in order to induce the specific biomaterial–biological interaction. Polyhydroxyalkanoate (PHAs) are of growing interests because of their natural origin, biodegradability, biocompatibility, and thermoplasticity; however, quite inert and intrinsic hydrophobic characteristics have hindered their extensive usage in medical applications. Surface modification of PHAs tailors the chemistry, wettability, and topography without altering the bulk properties, and introduces specific proteins/peptides and/or antibacterial agents to mediate cell–matrix interactions. This review describes the recent developments on the surface modification of PHAs to construct cell compatible and antibacterial surfaces.

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“…Thus, substrates of differing nanoscale topography that are otherwise viewed as “equivalent” in terms of compliance and bioactivity could potentially differ markedly with respect to the bioactivity of an adsorbed protein layer. A thorough review of existing literature indicates that this potentially critical confounding factor appears to be either universally unappreciated or discounted without appropriate experimental controls …”

Section: Introductionmentioning

confidence: 99%

Surface‐Driven Collagen Self‐Assembly Affects Early Osteogenic Stem Cell Signaling

Razafiarison

Silván

Meier

et al. 2016

Adv Healthcare Materials

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This study reports how extracellular matrix (ECM) ligand self-assembly on biomaterial surfaces and the resulting nanoscale architecture can drive stem cell behavior. To isolate the biological effects of surface wettability on protein deposition, folding, and ligand activity, a polydimethylsiloxane (PDMS)-based platform was developed and characterized with the ability to tune wettability of elastomeric substrates with otherwise equivalent topology, ligand loading, and mechanical properties. Using this platform, markedly different assembly of covalently bound type I collagen monomers was observed depending on wettability, with hydrophobic substrates yielding a relatively rough layer of collagen aggregates compared to a smooth collagen layer on more hydrophilic substrates. Cellular and molecular investigations with human bone marrow stromal cells revealed higher osteogenic differentiation and upregulation of focal adhesion-related components on the resulting smooth collagen layer coated substrates. The initial collagen assembly driven by the PDMS surface directly affected α1β1 integrin/discoidin domain receptor 1 signaling, activation of the extracellular signal-regulated kinase/mitogen activated protein kinase pathway, and ultimately markers of osteogenic stem cell differentiation. We demonstrate for the first time that surface-driven ligand assembly on material surfaces, even on materials with otherwise identical starting topographies and mechanical properties, can dominate the biomaterial surface-driven cell response.

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Biomaterial–stem cell interactions and their impact on stem cell response

Abstract: In this review, current research in the field of biomaterial properties for directing stem cells are discussed and placed in a critical perspective.

Cited by 45 publications

References 110 publications

Tyramine‐conjugated alginate hydrogels as a platform for bioactive scaffolds

Tyramine‐conjugated alginate hydrogels as a platform for bioactive scaffolds

Surface Modification of Polyhydroxyalkanoates toward Enhancing Cell Compatibility and Antibacterial Activity

Surface‐Driven Collagen Self‐Assembly Affects Early Osteogenic Stem Cell Signaling

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