Biomaterial 3D collagen I gel culture model: A novel approach to investigate tumorigenesis and dormancy of bladder cancer cells induced by tumor microenvironment

Qiu, Yueming; Qiu, Shi; Deng, Liangliang; Nie, Ling; Gong, Ling; Liao, Xinyang; Zheng, Xiaonan; Jin, Kun; Li, Jiakun; Tu, Xiang; Liu, Liangren; Liu, Zhenhua; Bao, Yige; Ai, Jianzhong; Lin, Tianhai; Yang, Lu; Wang, Qiang

doi:10.1016/j.biomaterials.2020.120217

Cited by 35 publications

(42 citation statements)

References 58 publications

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“…When treating the case of spheroids, which are known to be good tumor models [ 21 , 24 , 35 , 54 ], interesting data were found, regarding the role of collagen or the ECM [ 55 ]. Indeed, it is known that collagen can be synthesized by cells or can be recruited from the extracellular matrix, and in particular, collagen I was found to be a good model for the microenvironment of bladder cancer cells when studying tumorigenesis [ 33 ]. Using small amounts of collagen (here, ranging from 0 to 0.03 mg/mL), it was found that cells re-arrange efficiently in order to form a more compact structure (see Figure 5 ) using collagen as a proxy [ 24 , 56 ].…”

Section: Discussionmentioning

confidence: 99%

“…Then, ten-thousand cells were added into the well. Finally, type I rat tail collagen (Corning, Bedford, MA, USA) at a 0.01 or 0.03 mg/mL concentration (4

C and pH ∼ 7.4) was added to the medium, since collagen is a good model to mimic the microenvironment of bladder cancer cells [ 33 ]. The 96-well plate was centrifuged for 10 min at 200× g .…”

Section: Methodsmentioning

confidence: 99%

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Viscoelastic Properties in Cancer: From Cells to Spheroids

Abidine

Giannetti

Révilloud

et al. 2021

Cells

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AFM-based rheology methods enable the investigation of the viscoelastic properties of cancer cells. Such properties are known to be essential for cell functions, especially for malignant cells. Here, the relevance of the force modulation method was investigated to characterize the viscoelasticity of bladder cancer cells of various invasiveness on soft substrates, revealing that the rheology parameters are a signature of malignancy. Furthermore, the collagen microenvironment affects the viscoelastic moduli of cancer cell spheroids; thus, collagen serves as a powerful proxy, leading to an increase of the dynamic moduli vs. frequency, as predicted by a double power law model. Taken together, these results shed new light on how cancer cells and tissues adapt their viscoelastic properties depending on their malignancy and the microenvironment. This method could be an attractive way to control their properties in the future, based on the similarity of spheroids with in vivo tumor models.

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Section: Discussionmentioning

confidence: 99%

“…Then, ten-thousand cells were added into the well. Finally, type I rat tail collagen (Corning, Bedford, MA, USA) at a 0.01 or 0.03 mg/mL concentration (4

C and pH ∼ 7.4) was added to the medium, since collagen is a good model to mimic the microenvironment of bladder cancer cells [ 33 ]. The 96-well plate was centrifuged for 10 min at 200× g .…”

Section: Methodsmentioning

confidence: 99%

Viscoelastic Properties in Cancer: From Cells to Spheroids

Abidine

Giannetti

Révilloud

et al. 2021

Cells

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“…3D collagen gel culture was performed according previous report [ 33 ]. Briefly, type I collagen was diluted to 0.6 mg/ml with culture medium.…”

Section: Methodsmentioning

confidence: 99%

Type I collagen promotes tumor progression of integrin β1 positive gastric cancer through a BCL9L/β-catenin signaling pathway

Shan

Song

et al. 2021

Aging

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The mechanism of extracellular matrix induced tumor progression is poorly understood. Based on the TCGA database and clinical tumor tissues analysis, we observed abundant type I collagen expression in tumor tissues and poor overall survival in gastric patients with high integrin β1 (ITGB1) expression. In vitro , our study found that 3D collagen culture promoted the capability of colony formation and growth in ITGB1 positive gastric cancer, whereas limited colony growth was observed in ITGB1 negative gastric cancer, suggesting the role of ITGB1 in type I collagen associated tumor progression. Mechanistically, we demonstrated that type I collagen was capable of promoting the activation of BCL9L/β-catenin signaling pathway through ITGB1, thereby contributing to the gastric cancer development. Subsequently, β-catenin signals further up-regulated the expression anti-apoptosis protein BCL2, leading to the chemo-resistance in gastric cancer cells. Blockade of β-catenin signals efficiently improved the anticancer effects of chemotherapy, providing an innovative sight for clinical gastric cancer therapy.

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“…Upon ER stress, autophagy can recycle misfolded proteins and damaged organelles, thus promoting survival signaling and maintaining dormancy [91,92]. In addition, redox can activate autophagy through the integrins/PI3K/Akt/mTOR axis and transcriptional control, thereby managing dormancy induction [63,93,94]. Intriguingly, oxidative stress also inhibits autophagy by multiple mechanisms.…”

Section: Redox Sustaining Cancer Cell Quiescencementioning

confidence: 99%

Redox Control of the Dormant Cancer Cell Life Cycle

Huang

Hui

et al. 2021

Cells

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Following efficient tumor therapy, some cancer cells may survive through a dormancy process, contributing to tumor recurrence and worse outcomes. Dormancy is considered a process where most cancer cells in a tumor cell population are quiescent with no, or only slow, proliferation. Recent advances indicate that redox mechanisms control the dormant cancer cell life cycle, including dormancy entrance, long-term dormancy, and metastatic relapse. This regulatory network is orchestrated mainly through redox modification on key regulators or global change of reactive oxygen species (ROS) levels in dormant cancer cells. Encouragingly, several strategies targeting redox signaling, including sleeping, awaking, or killing dormant cancer cells are currently under early clinical evaluation. However, the molecular mechanisms underlying redox control of the dormant cancer cell cycle are poorly understood and need further exploration. In this review, we discuss the underlying molecular basis of redox signaling in the cell life cycle of dormant cancer and the potential redox-based targeting strategies for eliminating dormant cancer cells.

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Biomaterial 3D collagen I gel culture model: A novel approach to investigate tumorigenesis and dormancy of bladder cancer cells induced by tumor microenvironment

Cited by 35 publications

References 58 publications

Viscoelastic Properties in Cancer: From Cells to Spheroids

Viscoelastic Properties in Cancer: From Cells to Spheroids

Type I collagen promotes tumor progression of integrin β1 positive gastric cancer through a BCL9L/β-catenin signaling pathway

Redox Control of the Dormant Cancer Cell Life Cycle

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