Biomass to Xylose

Rashid, Rozina; Ejaz, Uroosa; Sohail, Muhammad

doi:10.1007/978-3-030-61837-7_15

Cited by 4 publications

(2 citation statements)

References 167 publications

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“…Exploring biodiversity in the search for new biocatalysts produced by indigenous microorganisms may permit the development of biocatalysts at the commercial level. Previously, various microbial strains, including Penicillium sp., Aspergillus niger , Bacillus sp., Thermobacillus xylanilyticus , Aspergillus oryzae and Aspergillus foetidus , have been reported to produce xylanase [ 20 ]. In spite of the availability of high-throughput genetic engineering techniques, the isolation of novel indigenous strains with the ability to overproduce metabolites of commercial importance has remained at the core of industrial microbiology research.…”

Section: Resultsmentioning

confidence: 99%

Utilization of Corncob as an Immobilization Matrix for a Xylanolytic Yeast Strain

et al. 2023

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Immobilization of microbial cells for the production of industrially important enzymes has been reported to offer the advantages of recyclability, higher yields and cost effectiveness. The search for an appropriate matrix that is affordable and easy to prepare is a significant topic in microbial biotechnology. Here, an abundant type of agro-industrial waste—corncob—was utilized as an immobilization matrix for the production of xylanase from an indigenous yeast strain, Saccharomyces cerevisiae MK-157. This is the first report describing xylanase production from immobilized S. cerevisiae. To render the corncob matrix more porous, alkaline pretreatment was undertaken and yeast cells were immobilized on the matrix by cultivating at 30 °C for 48 h in Sabouraud dextrose broth. After incubation, the immobilized matrix was transferred to mineral salt medium containing 1% xylan and incubated at 30 °C for 24 h. Xylanase production was determined in cell-free culture supernatant and the matrix was recycled for up to seven cycles. Moreover, xylanase-mediated saccharification was carried out using sugarcane bagasse as a substrate and the release of reducing sugars was monitored. The results showed that the immobilized yeast produced 4.97 IU mL−1 xylanase in the first production cycle, indicating a >tenfold increase compared to the free cells. Xylanase production further increased to its maximum levels (9.23 IU mL−1) in the fourth production cycle. Nonetheless, the cells retained 100% productivity for up to seven cycles. The volumetric and specific productivity of xylanase were also the highest in the fourth cycle. Scanning electron microscopy images revealed the rough surface of the untreated corncob, which became more porous after alkaline pretreatment. Immobilized yeast cells were also visible on the corncob pieces. The saccharification of a natural resource—sugarcane bagasse—using xylanase preparation yielded 26 mg L−1 of reducing sugars. Therefore, it can be concluded that yeast strains can yield sufficient quantities of xylanase, allowing possible biotechnological applications. Moreover, corncob can serve as a cost-effective matrix for industrially important yeast strains.

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Section: Resultsmentioning

confidence: 99%

Utilization of Corncob as an Immobilization Matrix for a Xylanolytic Yeast Strain

et al. 2023

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“…Moreover, xylanase is used for clarification of juices and wine, treatment of agricultural wastes and de-inking of wastepaper. This enzyme is also employed in different industries including textile, leather, paper and pulp, ethanol production, and pharmaceutical industries [13]. However, presence of lignin in SB hinders the access of substrates to their respective enzymes and inhibiting the effective conversion of cellulose and hemicellulose into monomeric sugars [14,15].…”

mentioning

confidence: 99%

Toward a zero waste approach: Utilization of sugarcane bagasse for dye removal and multienzymes production

Ejaz,

Taj,

Sohail

et al. 2023

J Basic Microbiol

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Global production of sugarcane bagasse (SB) by sugar industries exceeds more than 100 tons per annum. SB is rich in lignin and polysaccharide and hence can serve as a low‐cost energy and carbon source for the growth of industrially important microorganism. However, various other applications of SB have also been investigated. In this study, SB was used as an adsorbent to remove an azo dye, malachite green. Subsequently, the dye‐adsorbed SB was fermented by Trametes pubescens MB 89 for the production of laccase enzyme. The fungal pretreated SB was further utilized as a substrate for the simultaneous production of multiple plant cell wall degrading enzymes including, cellulase, xylanase, pectinase, and amylase by thermophilic bacterial strains. Results showed that 0.1% SB removed 97.04% malachite green at 30°C after 30 min from a solution containing 66 ppm of the dye. Fermentation of the dye‐adsorbed SB by T. pubescens MB 89 yielded 667.203 IU mL−1 laccase. Moreover, Brevibacillus borstelensis UE10 produced 38.41 and 18.6 IU mL−1 β‐glucosidase and pectinase, respectively, by using fungal‐pretreated SB. Cultivation of B. borstelensis UE27 in the medium containing the same substrate yielded 32.14 IU mL−1 of endoglucanase and 27.23 IU mL−1 of β‐glucosidase. Likewise, Neobacillus sedimentimangrovi UE25 could produce a mix of β‐glucosidase (37.24 IU mL−1), xylanase (18.65 IU mL−1) and endoglucanase (26.65 IU mL−1). Hence, this study led to the development of a method through which dye‐containing textile effluent can be treated by SB along with the production of industrially important enzymes.

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Characterization of a novel end product tolerant and thermostable cellulase from Neobacillus sedimentimangrovi UE25

Ejaz

Moin

Sohail

et al. 2023

Enzyme and Microbial Technology

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Biomass to Xylose

Cited by 4 publications

References 167 publications

Utilization of Corncob as an Immobilization Matrix for a Xylanolytic Yeast Strain

Utilization of Corncob as an Immobilization Matrix for a Xylanolytic Yeast Strain

Toward a zero waste approach: Utilization of sugarcane bagasse for dye removal and multienzymes production

Characterization of a novel end product tolerant and thermostable cellulase from Neobacillus sedimentimangrovi UE25

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