Biomass production from glutamate fermentation wastewater by the co-culture of Candida halophila and Rhodotorula glutinis

Abstract

Conversion of oil-rich salad oil manufacturing wastewater (SOMW) into protein source for animal feed through biomass production of yeast isolate was investigated in this study. Five species of yeasts, including Rhodotorula rubra, Candida tropicalis, C. utilis, C. boidinii, Trichosporon cutaneum, were isolated from SOMW following enrichment culture. Of them, C. utilis was chosen as the sole biomass producer in the study due to its greatest oil uptake rate, 0.96 kg oil kg À1 biomass d À1 , and highest specific growth rate, 0.25 h À1 . The cells of C. utilis contained 26% protein, 9% crude lipid, 55% carbohydrate and balanced amino acid compositions. The initial N:C ratio in SOMW drastically influenced oil reduction efficiency, biomass production and protein content of C. utilis, and therefore a range between 1:6 and 1:8 was recommended in consideration of these three factors simultaneously.

Production ofCandidaBiomass from Hydrolysed Agricultural Biowaste

“…Candida sp. is one of the most widely used organisms in the ScP production (1,2,15,16,20,21). over the years efforts have been made to use agricultural residues for production of microbial biomass.…”

Aproveitamento do melaço e vinhaça de cana-de-açúcar como substrato para produção de biomassa protéica e lipídica por leveduras e bactéria

“…Entre os subprodutos mais abundantes, podem ser citados: o soro de queijo ou de leite (PARASKEVOPOULOU et al, 2003), e os resíduos da produção do glutamato (ZHENG et al, 2005), material celulósico de origem vegetal (DIAZ et al, 2003). Também merecem realce o melaço e a vinhaça de cana-de-açúcar, que podem ser empregados para o desenvolvimento de produtos biotecnológicos de interesse industrial (ALTAF et al, 2005) por muitos microrganismos como leveduras, algas, fungos e bactérias (ARNOLD; KNAPP; JOHNSON, 2000;ANUPAMA;RAVINDRA, 2000).…”

Effect of mineral salts and nitrogen source on yeast (Candida utilis NOY1) biomass production using tubers wastes

“…Yeasts are common microorganisms which can grow on fruit and vegetable processing wastes. Several studies were done on bio-transformation of different agricultural waste into value-added products such as yeast biomass from salad oil manufacturing wastewater (Zheng et al, 2005a), ethanol production from non-sterilized beet molasses (Patrascu et al, 2009), biomass production from rice straw and tubers wastes hydrolysate (Zheng et al, 2005b;Ouédraogo et al, 2012a), and cultivation of the yeast on pineapple cannery effluent (Rosma et al, 2005). Yeast from genera Candida, Saccharomyces, Torulopsis and Lipomyces are able to transfer carbon source from carbohydrate to lipid, while the protoplasm contains high quantity of glyceride, phospholipid and ergosterol.…”