Biomass production from Bacillus sp. RAB9 using several carbon sources

Sousa, C.M.R. de; Klainer, Bruno; Lima, Karine Thiers Leitão; Pinto, Gustavo Adolfo Saavedra

doi:10.1186/1753-6561-8-s4-p172

Cited by 2 publications

(2 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the significant cost of glucose in the total cost of mixotrophic algal biomass production is a major drawback. Low-cost carbon source from industrial and agricultural waster such as corn powder hydrolysate and sugar cane molasses has been employed to replace glucose in algal mixotrophic growth ( Xu et al, 2006 ; Sousa et al, 2014 ). While algae-based lipid is suitable for biodiesel production, algae-based starch is a useful feedstock for bioethanol production ( Chisti, 2007 ; Hu et al, 2008 ; Ho et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome and Metabolome Profiling of a Novel Isolate Chlorella sorokiniana G32 (Chlorophyta) Displaying Enhanced Starch Accumulation at High Growth Rate Under Mixotrophic Condition

et al. 2022

View full text Add to dashboard Cite

Chlorella sorokiniana is one of the most productive microalgal species with a high potential for the production of biofuels and other high value-added molecules. Many studies have focused on its capability of mixotrophic growth using reduced organic carbon and growth pattern shift between autotrophic and mixotrophic conditions. In this study, we investigated growth patterns of a novel isolate, C. sorokiniana G32, under mixotrophic growth conditions supplemented with a low level (1.25 g L–1) and a high level (5 g L–1) of glucose. Physiological, transcriptomic (i.e., RNA-seq), and metabolomic (i.e., LC-MS/MS) methods were used. We showed that peak growth based on OD680nm absorbance is ∼4-fold higher with high glucose vs. low glucose supplementation. Photosynthetic efficiency (Fv/Fm) in G32 mixotrophic cultures with high or low glucose supplementation remains identical to that of G32 phototrophic growth. We also found that the conversion rate between absorbance-based cell density and cell dry weight with high glucose supplementation was lower than with low glucose. This suggests that more cell biomass is produced under high glucose treatment than with low glucose. The result was confirmed via sucrose density gradient centrifugation. It is likely that accumulation of high concentration of starch may account for this effect. Transcriptomic analysis of G32 cultures (i.e., via RNA-seq) in response to reciprocal change of glucose levels reveals that expression of a subset of differentially expressed genes (DEGs) is correlated with the amount of glucose supplementation. These DEGs are designated as glucose-specific responsive (GSR) genes. GSR genes are enriched for a number of energy metabolic pathways. Together with metabolomics data (i.e., LC-MS/MS), we show that under high-level supplementation, glucose is preferentially oxidized through an oxidative pentose phosphate pathway. Collectively, our results indicate the mechanism of regulation of glucose assimilation and energy metabolism in G32 under mixotrophic conditions with different levels of glucose supplementation revealed by transcriptomic and metabolomic analyses. We propose that C. sorokiniana G32 has the potential for the production of high value-added molecules.

show abstract

Section: Discussionmentioning

confidence: 99%

Transcriptome and Metabolome Profiling of a Novel Isolate Chlorella sorokiniana G32 (Chlorophyta) Displaying Enhanced Starch Accumulation at High Growth Rate Under Mixotrophic Condition

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The composition of the nutrient medium has a great influence on the microbial growth. Various components usually used for culture of Bacillus [14,15], among them, naturally derived nutrient such as glucose, malt extract, peptone, meat extract and yeast extract were the most components used as carbon and nitrogen sources. However, the effect of each ingredient on microbial growth are still controversial.…”

Section: Screening Of Culture Medium Components For B Clausii Growthmentioning

confidence: 99%

Improvement of Bacillus clausii cell HH1 density via culture medium optimization and pH-stat fed batch fermentation

Pham,

Nguyen,

2023

Vietnam J. Sci. Technol.

View full text Add to dashboard Cite

In this study, the culture medium and fermentation modes were studied aiming to improve the cell density of Bacillus clausii. Firstly, the factorial design method using Minimum Run Resolution IV design was used to evaluate the relative importance of culture medium components to the growth of Bacillus clausii. The results showed that three components peptone, yeast extract and malt extract were the components significantly affecting the bacterial biomass. Then, the optimization of these three ingredient concentrations using a response surface methodology with the Box-Behnken design resulted that the maximal biomass had been achieved using the medium containing 7.64 g/L peptone, 10 g/L yeast extract and 6.36 g/L malt extract. Finally, the pH-stat fed batch fermentation was conducted in a 2-liter bioreactor where the 9X concentrated optimal culture medium was feed into the bioreactor based on the pH signal. As a result, the microbial cell density increased by 2.9-fold compared to that achieved through batch fermentation.

show abstract

Biomass production from Bacillus sp. RAB9 using several carbon sources

Cited by 2 publications

References 4 publications

Transcriptome and Metabolome Profiling of a Novel Isolate Chlorella sorokiniana G32 (Chlorophyta) Displaying Enhanced Starch Accumulation at High Growth Rate Under Mixotrophic Condition

Transcriptome and Metabolome Profiling of a Novel Isolate Chlorella sorokiniana G32 (Chlorophyta) Displaying Enhanced Starch Accumulation at High Growth Rate Under Mixotrophic Condition

Improvement of Bacillus clausii cell HH1 density via culture medium optimization and pH-stat fed batch fermentation

Contact Info

Product

Resources

About