Biomass composition of two new energy cane cultivars compared with their ancestral Saccharum spontaneum during internode development

Fanelli, Amanda; Reinhardt, Laurie A.; Matsuoka, S.; Ferraz, André; Silva, Tatiane da Franca; Hatfield, Ronald D.; Romanel, Elisson

doi:10.1016/j.biombioe.2020.105696

Cited by 8 publications

(3 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, p CA is predominantly ester-linked to lignin, mostly to S units ( Ralph et al, 1994a ; Hatfield et al, 2009 ; Ralph, 2010 ). Among widely cultivated crops, Saccharum spp., sorghum, and maize are particularly good sources of p CA, as they have high levels of p CA in their culms, ranging from 3 to 6% of the dry weight, predominating over (releasable) FA (0.5–2% weight) ( Masarin et al, 2011 ; del Río et al, 2015 ; Hatfield et al, 2017 ; Fanelli et al, 2020 ). Furthermore, these are C4 grasses, with highly efficient photosynthetic rates, having great potential as bioenergy crops ( Carpita and McCann, 2008 ; van der Weijde et al, 2013 ; Hoang et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of a Sugarcane BAHD Acyltransferase Alters Hydroxycinnamate Content in Maize Cell Wall

Fanelli

Rancour²,

Sullivan

et al. 2021

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

The purification of hydroxycinnamic acids [p-coumaric acid (pCA) and ferulic acid (FA)] from grass cell walls requires high-cost processes. Feedstocks with increased levels of one hydroxycinnamate in preference to the other are therefore highly desirable. We identified and conducted expression analysis for nine BAHD acyltransferase ScAts genes from sugarcane. The high conservation of AT10 proteins, together with their similar gene expression patterns, supported a similar role in distinct grasses. Overexpression of ScAT10 in maize resulted in up to 75% increase in total pCA content. Mild hydrolysis and derivatization followed by reductive cleavage (DFRC) analysis showed that pCA increase was restricted to the hemicellulosic portion of the cell wall. Furthermore, total FA content was reduced up to 88%, resulting in a 10-fold increase in the pCA/FA ratio. Thus, we functionally characterized a sugarcane gene involved in pCA content on hemicelluloses and generated a C4 plant that is promising for valorizing pCA production in biorefineries.

show abstract

Section: Introductionmentioning

confidence: 99%

Overexpression of a Sugarcane BAHD Acyltransferase Alters Hydroxycinnamate Content in Maize Cell Wall

Fanelli

Rancour²,

Sullivan

et al. 2021

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…[8] Sugarcane is constantly improving, aiming at its energy use, through the crossing of species that give the final product greater resistance, higher productivity and higher fiber content [9]. This process leads to the production of "energy cane" through crosses between genera and species such as Erianthus, Miscanthus, Narenga, Sclerostachya and other Saccharum [10].…”

Section: Introductionmentioning

confidence: 99%

Liquid chromatographic fractionation of bio-oil from sugarcane bagasse: influence of heating rate on bio-oil yield and quality

Conrado,

Cardoso,

Farrapeira

et al. 2024

Cad. Pedagógico

View full text Add to dashboard Cite

Brazil stands out in the field of using biomass as a source of energy and biomaterials, due to its territorial extension, biodiversity and climatic conditions. In this sense, one can highlight the high potential of bio-products generated from biomass. This is the case of sugarcane bagasse, produced in large quantities allied to the sugar and alcohol industry. In Brazil, much research has been done to improve its amount of fiber, aiming at the production of alternative fuels and generating the so-called "energy cane". Thus, it was studied the use of sugarcane bagasse for the production of bio-oil through pyrolysis and the isolation of fractions for industrial application. A comparison was also made between two types of sugarcane, a commercial variety (Saccharum sp.) and a variety with some genetic improvement (Erianthus arundinaceus). The final pyrolysis temperature was set at 500 ° C by varying the heating rates (25, 45, and 65 ° C min-1). The bio-oils were fractionated using preparative liquid chromatography and their fractions were analyzed by gas chromatography coupled to mass spectrometry. The fractionation of bio-oils increased the number of compounds identified by about 50%, besides allowing the isolation of apolar compounds. In addition, it was found that the genetic improved sugarcane presented higher bio-oil content, with higher hydrocarbon content, when compared to commercial sugarcane, demonstrating that the improvement process was efficient. Among the compounds identified were phenols, furfural derivatives and hydrocarbons, which indicates the potential use of bio-oil not only as bio-fuels, but also for industrial purposes.

show abstract

“…Renewable resources, such as lignocellulosic biomass, have been gaining prominence in the production of energy and high-valueadded inputs (MATEI, et al, 2020). Within this focus, the conversion of these biomasses into second-generation bioethanol has been developing and being applied industrially (FANELLI, et al, 2020;SOLARTE-TORO, et al, 2019). This industrial field is increasingly connected to developing more efficient physical-chemical and biochemical processes in the production of biofuels, aiming at lower consumption of energy, water, chemical inputs and generation of waste from pre-treatments and subsequent treatments that may cause damage to the environment.…”

Section: Introductionmentioning

confidence: 99%

Pre-Treatment With Hydrochloric Acid and Enzymatic Hydrolysis of Sugar Cane Bagass to Obtain Glucose

Abreu,

Silva

2023

JER

View full text Add to dashboard Cite

Pretreatment and enzymatic hydrolysis are two important steps within the process of converting lignocellulosic biomass into glucose. In this work, sugarcane bagasse (BCA) ground into particles smaller than 40 mesh (< 425 µm) was pre-treated with hydrochloric acid (HCl) in different concentrations (0.75, 1.0 and 1.25 mol/L), in time varying from 30 to 90 min by autoclaving (121 ºC / 1 atm) in solid ratio :net 1:10. The pretreated BCA was subjected to hydrolysis with commercial enzymes at a solid:liquid ratio of 1:30, with100 µL of cellulase mixtures and 100 µL of Aspergilus niger laccase, in citrate-phosphate buffer (50 mM; pH 4.8) for 72h at 50 ºC under agitation at 150 rpm. Pre-treatment with HCl showed an efficiency in delignification of BCA of 59.2% and the glycan fraction increased to 64.48% in the solid residue of BCA recovered after pretreatment carried out with HCl 1.25 mol/L, time 30 min. Within these operational parameters, enzymatic hydrolysis continued for 72h. Within a mass balance analysis, a solids recovery of 42.8% was observed for the pre-treatment stage with HCl. Therefore, for every 100 g of pre-treated BCA, within the process, residue with 64.48 g of glycan and 9.23 g of lignin was recovered and for every 100 g of BCA recovered after pre-treatment and enzymatically treated with cellulases and laccase it was possible to obtain 12.0g of glycan hydrolyzed into glucose. It is concluded that pre-treatment with dilute acid and hydrolysis with commercial enzymes showed efficiency in converting BCA glycan into glucose.

show abstract

Biomass composition of two new energy cane cultivars compared with their ancestral Saccharum spontaneum during internode development

Cited by 8 publications

References 35 publications

Overexpression of a Sugarcane BAHD Acyltransferase Alters Hydroxycinnamate Content in Maize Cell Wall

Overexpression of a Sugarcane BAHD Acyltransferase Alters Hydroxycinnamate Content in Maize Cell Wall

Liquid chromatographic fractionation of bio-oil from sugarcane bagasse: influence of heating rate on bio-oil yield and quality

Pre-Treatment With Hydrochloric Acid and Enzymatic Hydrolysis of Sugar Cane Bagass to Obtain Glucose

Contact Info

Product

Resources

About