Biomass and lipid characterization of microalgae genera Botryococcus, Chlorella, and Desmodesmus aiming high-value fatty acid production

Ferreira, Gabriela F.; Pinto, Luisa Fernanda Ríos; Carvalho, Patrícia de Oliveira; Coelho, Mirela B.; Eberlin, Marcos N.; Filho, Rubens Maciel; Fregolente, Leonardo Vasconcelos

doi:10.1007/s13399-019-00566-3

Cited by 43 publications

(15 citation statements)

References 98 publications

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“…The carbohydrates content (58% (w/w) of CDW) of optimized biomass was found comparably higher than carbohydrates content of strains reported earlier such as Ulva fasciata (46.96%), Chlorella salina (50%) ( Barakat et al, 2021 , Mayers et al, 2018 ) and comparable with the highest carbohydrate content of 49% and 50% reported for commercially important microalgae Chlamydomonas sp . ( Morales-Sánchez et al, 2020 ) and Chlorella vulgaris ( Ferreira et al, 2019 ) respectively. The high carbohydrate content is favorable for providing the amount of fermentable sugars for ethanol production.…”

Section: Discussionmentioning

confidence: 99%

Deciphering role of technical bioprocess parameters for bioethanol production using microalgae

Bibi

Yasmin

Jamal

et al. 2021

Saudi Journal of Biological Sciences

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Microalgae biomass is considered an important feedstock for biofuels and other bioactive compounds due to its faster growth rate, high biomass production and high biomolecules accumulation over first and second-generation feedstock. This research aimed to maximize the specific growth rate of fresh water green microalgae Closteriopsis acicularis, a member of family Chlorellaceae under the effect of pH and phosphate concentration to attain enhanced biomass productivity. This study investigates the individual and cumulative effect of phosphate concentration and pH on specific growth characteristics of Closteriopsis acicularis in autotrophic mode of cultivation for bioethanol production. Central-Composite Design (CCD) strategy and Response Surface Methodology (RSM) was used for the optimization of microalga growth and ethanol production under laboratory conditions. The results showed that high specific growth rate and biomass productivity of 0.342 day −1 and 0.497 g L −1 day −1 respectively, were achieved at high concentration of phosphate (0.115 g L −1 ) and pH (9) at 21st day of cultivation. The elemental composition of optimized biomass has shown enhanced elemental accumulation of certain macro (C, O, P) and micronutrients (Na, Mg, Al, K, Ca and Fe) except for nitrogen and sulfur. The Fourier transform infrared spectroscopic analysis has revealed spectral peaks and high absorbance in spectral range of carbohydrates, lipids and proteins, in optimized biomass. The carbohydrates content of optimized biomass was observed as 58%, with 29.3 g L −1 of fermentable sugars after acid catalyzed saccharification. The bioethanol yield was estimated as 51 % g ethanol/g glucose with maximum of 14.9 g/L of bioethanol production. In conclusion, it can be inferred that high specific growth rate and biomass productivity can be achieved by varying levels of phosphate concentration and pH during cultivation of Closteriopsis acicularis for improved yield of microbial growth, biomass and bioethanol production.

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Section: Discussionmentioning

confidence: 99%

Deciphering role of technical bioprocess parameters for bioethanol production using microalgae

Bibi

Yasmin

Jamal

et al. 2021

Saudi Journal of Biological Sciences

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“…They mainly focus on the FA composition and aim to establish a relationship between the FA profile and PUFA abundance with antioxidant activity [70]. Algae extracts are complex mixtures that comprise different classes of lipids, including neutral lipids, such as triacylglycerol (TAG), sterols, free FA; and polar lipids, such as phospholipids, glycolipids, and betaine lipids [73][74][75][76][77]. Recently, it has been suggested that polar lipids such as phospholipids, are better at delivering omega-3 PUFA [78].…”

Section: Algae Lipids With Antioxidant Activitymentioning

confidence: 99%

Algal Lipids as Modulators of Skin Disease: A Critical Review

et al. 2022

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The prevalence of inflammatory skin diseases continues to increase with a high incidence in children and adults. These diseases are triggered by environmental factors, such as UV radiation, certain chemical compounds, infectious agents, and in some cases, people with a genetic predisposition. The pathophysiology of inflammatory skin diseases such as psoriasis or atopic dermatitis, but also of skin cancers, is the result of the activation of inflammation-related metabolic pathways and the overproduction of pro-inflammatory cytokines observed in in vitro and in vivo studies. Inflammatory skin diseases are also associated with oxidative stress, overproduction of ROS, and impaired antioxidant defense, which affects the metabolism of immune cells and skin cells (keratinocytes and fibroblasts) in systemic and skin disorders. Lipids from algae have been scarcely applied to modulate skin diseases, but they are well known antioxidant and anti-inflammatory agents. They have shown scavenging activities and can modulate redox homeostasis enzymes. They can also downmodulate key inflammatory signaling pathways and transcription factors such as NF-κB, decreasing the expression of pro-inflammatory mediators. Thus, the exploitation of algae lipids as therapeutical agents for the treatment of inflammatory skin diseases is highly attractive, being critically reviewed in the present work.

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“…SD, RAW and RHW supervised sterols and vitamins has attracted the attention of biotechnological, pharmaceutical, cosmetic, biofuel, food and feed industries (Chisti, 2007;Christaki et al, 2011;Draaisma et al, 2013;Pulz & Gross, 2004). Nevertheless, bioprospecting continues and researchers are characterizing new microalgae species in terms of growth and lipid composition (Barkia et al, 2019;Breuer et al, 2012;Duong et al, 2015;Ferreira et al, 2019;Gim et al, 2014;Lim et al, 2012).…”

Section: Authors Contributionsmentioning

confidence: 99%

Genetic engineering of microalgae for enhanced lipid production

Muñoz

Südfeld

Naduthodi

et al. 2021

Biotechnology Advances

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Genetic transformation of microalgae remains a challenge due to poor intracellular delivery of exogenous molecules. This limitation is caused by the structure and composition of the cell wall and cell membrane of each species.Moreover, successful delivery of proteins or nucleic acids cannot be assessed by determining transformability since their functionality is not always known in the studied microorganism. We propose a quick and effective screening tool for the prediction and optimization of electroporation settings by monitoring cell permeability and viability using Sytox Green and propidium iodide respectively.We determined voltage settings for the microalgae Chlamydomonas reinhardtii, Chlorella vulgaris, Neochloris oleoabundans and Acutodesmus obliquus. To evaluate the predicted settings, we delivered labelled DNA and proteins into the cells. We demonstrated that high transformation efficiencies can be accomplished when predicted values were applied with functional plasmids.Additionally, we increased transformation efficiencies by testing cell concentrations, light intensities and fragment sizes. This method can be used to determine suitable transformation conditions for non-transformed microalgae species and to increase the insight on established transformation protocols.

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Biomass and lipid characterization of microalgae genera Botryococcus, Chlorella, and Desmodesmus aiming high-value fatty acid production

Cited by 43 publications

References 98 publications

Deciphering role of technical bioprocess parameters for bioethanol production using microalgae

Deciphering role of technical bioprocess parameters for bioethanol production using microalgae

Algal Lipids as Modulators of Skin Disease: A Critical Review

Genetic engineering of microalgae for enhanced lipid production

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