Biomarkers of oxidative stress in the land snail, Theba pisana for assessing ecotoxicological effects of urban metal pollution

Radwan, Mohamed; El‐Gendy, K.S.; Gad, Amira F.

doi:10.1016/j.chemosphere.2010.01.056

Cited by 91 publications

(54 citation statements)

References 34 publications

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“…According to Lukkari, Taavitsainen, Vaisanen, and Haimi (2009), the role of GST is to detoxify electrophile intermediate compounds by conjugation with glutathione and several xenobiotics and heavy metals induced increased activity of the GST system in exposed organisms. The significant increased GST activity observed in this work may be related to free radical reactions, resulting in the induction of metal-binding protein and the subsequent metal sequestering, to result in its presence in a biologically inactive form, thus enabling the survival of the organism (Radwan, El-Gendy, & Gad, 2010). The observed increase in GST activity in this study agreed with the work of Saint-Denis, Narbonne, Arnaud, Thybaud, and Ribera (1999), who observed increase in GST activity in earthworms exposed to benzo(a)pyrene after three weeks of exposure.…”

Section: Discussionmentioning

confidence: 99%

Glutathione-S-Transferase Production in Earthworm as Tool for Assessment of Heavy Metal Pollution in Abattoir Soil

Owagboriaye

Dedeke

Ademolu

2016

RBT

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Abstract:The use of direct response of animals to environmental challenges by production of biomarkers is a better tool to assess environmental pollution than the conventional methods. This study aimed to measure Glutathione-S-transferase (GST) in earthworms as tools for assessing heavy metal pollution in abattoir soil. Five (5) replicates each of earthworm species (Libyodrilus violaceous, Eudrilus eugeniae and Alma millsoni), soil and rumen waste samples were collected from three (3) abattoir sites (Lafenwa, Gbonogun and Madojutimi abattoirs), and a control site located within Federal University of Agriculture Abeokuta, beside an undisturbed stream with no rumen waste. Heavy metal (Cu, Zn, Pb, Cd, Co, Cr, Ni and Mn) concentrations in rumen waste, abattoir soils and earthworm tissues were determined using Atomic Absorption Spectrophotometer. The pH and organic matter (OM) concentrations of the rumen waste and abattoir soils were determined by standard methods. GST activities in the earthworm tissues were determined through the conjugation of 1 mM reduced glutathione (GSH) with 1 mM 1-chloro-2,4-dinitrobenzene (CDNB). The rumen waste recorded significantly higher (p ≤ 0.05) % OM, heavy metal concentrations and pH level than in their respective abattoir soils. The mean heavy metal concentrations of Cu, Zn, Pb, Cd and Mn were highest in the tissue of earthworm species obtained from Lafenwa abattoir. A significantly (p ≤ 0.05) higher GST activities were recorded in the tissue of earthworm species obtained from Lafenwa and Gbonogun abattoirs. Libyodrilous violaceus obtained from Lafenwa abattoir recorded the highest GST activity (8.47±1.39) in their tissue followed by the ones from Gbonogun abattoir (8.21±0.85). A significant (p ≤ 0.05) positive correlations was observed between GST activities in earthworm tissues and heavy metal concentrations. GST activities can therefore be used to assess the level of heavy metal pollution in abattoir soils. Rev. Biol. Trop. 64 (2): 779-789. Epub 2016 June 01.

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Section: Discussionmentioning

confidence: 99%

Glutathione-S-Transferase Production in Earthworm as Tool for Assessment of Heavy Metal Pollution in Abattoir Soil

Owagboriaye

Dedeke

Ademolu

2016

RBT

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“…Heavy metal bioaccumulations in gastropods tissues are generally dependent on metal, species (Liang et al 2004), physiological condition, and the habitat environment (Cravo & Bebianno 2005). Many studies have shown that land snails can be appropriate biomonitoring organisms (Otitoloju et al 2009;Berandah et al 2010;Radwan et al 2010;Anim et al 2011;Itziou & Dimitriadis 2011).…”

Section: Discussionmentioning

confidence: 99%

“…However, metal levels in Helix aspersa collected from five locations in Italy ranged between 8.65-75.0 µg/g for Cu, 1.62-80.5 µg/g for Pb, 126-514 µg/g for Zn and 5.61-8.93 µg/g for Cd (Regoli et al 2006). The metal levels in Theba pisana collected from six sites in Alexandria, Egypt, ranged between 48.5-357.3 µg/g for Cu, 6.86-28.8 µg/g for Pb, 0.002-0.001 µg/g for Cd and 123.1-392.5 µg/g for Zn (Radwan et al 2010). The differences in heavy metal levels between the present study and previous investigations (Regoli et al 2006;Radwan et al 2010) could be due to the tested snail species, animal body size, the distance from the major road where the snails were collected and the type of emissions in the environment of the studied area.…”

Section: Discussionmentioning

confidence: 99%

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Bioaccumulation and histopathological changes of the digestive gland of the land snailEobania vermiculata(Mollusca: Gastropoda), as biomarkers of terrestrial heavy metal pollution in Taif city

Mohammadein¹,

El-Shenawy²,

AL-Fahmie³

2013

Italian Journal of Zoology

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“…Aside of these enzymes, there are much more antioxidants acting as free radical scavengers or substrates. For example, most importantly reduced glutathione or other enzymes like glutathione reductase (GSR) or glutathione-S-transferase (GST), which additionally need reduced glutathione as a cofactor for their activity (Meister 1988;Pannunzio and Storey 1998;Radwan et al 2010). In the context of our results, we assume that one or several enzymes of the antioxidant defence system may have an activity optimum or are expressed in a higher amount at low temperatures (25 and 33°C), in the following called 'defence mechanism 1'.…”

Section: Fox Assaymentioning

confidence: 99%

Hsp70 and lipid peroxide levels following heat stress in Xeropicta derbentina (Krynicki 1836) (Gastropoda, Pulmonata) with regard to different colour morphs

Dieterich

Troschinski

Schwarz

et al. 2015

Cell Stress and Chaperones

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Terrestrial snails which live under dry and hot conditions need efficient mechanisms of adaptation to counteract the problems of desiccation and over-heating. A profoundly heat tolerant snail species is the Mediterranean Xeropicta derbentina, exhibiting different shell colour morphs ranging from pale white to darkly banded. Considering that dark-pigmented snails are believed to have a disadvantage due to faster heating, we investigated possible differences in the stress markers Hsp70 and lipid peroxideation between four pre-defined colour morphs which were exposed to different temperatures for eight hours. The highest Hsp70 levels were observed in response to 38-40°C. Levels decreased when this temperature was exceeded. Snails of a pre-defined colour category 3 (with a large black band at the umbilicus side of the shell) showed the most prominent Hsp70 response. Lipid peroxideation levels also showed a maximum at 38°C but displayed a second peak at rather high temperatures at which the Hsp70 level already had decreased (45-48°C). Particularly pure white snails (category 1) and the most pigmented ones (category 4) were found to have different levels of lipid peroxidation at 38°C and 45°C compared to the other morphs. A hypothesis involving a combined two-phase defence mechanism, to which both, the Hsp70 protection system and the antioxidant defence system, may contribute, is discussed.

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Biomarkers of oxidative stress in the land snail, Theba pisana for assessing ecotoxicological effects of urban metal pollution

Cited by 91 publications

References 34 publications

Glutathione-S-Transferase Production in Earthworm as Tool for Assessment of Heavy Metal Pollution in Abattoir Soil

Glutathione-S-Transferase Production in Earthworm as Tool for Assessment of Heavy Metal Pollution in Abattoir Soil

Bioaccumulation and histopathological changes of the digestive gland of the land snailEobania vermiculata(Mollusca: Gastropoda), as biomarkers of terrestrial heavy metal pollution in Taif city

Hsp70 and lipid peroxide levels following heat stress in Xeropicta derbentina (Krynicki 1836) (Gastropoda, Pulmonata) with regard to different colour morphs

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