Biomarkers of oxidative stress in erythrocytes as a function of human age

Maurya, Pawan Kumar; Kumar, Prabhanshu; Chandra, Pranjal

doi:10.5662/wjm.v5.i4.216

Cited by 88 publications

(70 citation statements)

References 61 publications

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“…The process of ageing is in general characterized by the increased level of oxidative stress [35]. Numerous studies showed a positive correlation between resistance to oxidative stress and maximal lifespan in a variety of mammals, from hamsters to humans [24, 33].…”

Section: Discussionmentioning

confidence: 99%

Expression of cellular protective proteins SIRT1, HSP70 and SOD2 correlates with age and is significantly higher in NK cells of the oldest seniors

et al. 2017

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BackgroundNK cells are key effector lymphocytes of innate immunity provided with constitutive cytolytic activity, however, their role in human ageing is not entirely understood. The study aimed to analyze the expression of proteins involved in cellular stress response sirtuin 1 (SIRT1), heat shock protein 70 (HSP70) and manganese superoxide dismutase (SOD2) in non-stimulated NK cells of the oldest seniors (n = 25; aged over 85; mean age 88 years) and compare with NK cells of the old (n = 30; aged under 85; mean age 76 years) and the young (n = 32; mean age 21 years) to find potential relationships between the level of expression of these proteins in NK cells and longevity. The concentration of carbonyl groups and 8-isoprostanes in NK cell lysates reflecting the level of oxidative stress was also measured.ResultsThe group of the oldest seniors differed from the other age groups by significantly higher percentage of NK cells expressing SIRT1, HSP70 and SOD2. The concentration of both carbonyl groups and 8-isoprostanes in NK cell extracts remained within the normal range in all age groups. The percentage of NK cells with the expression of, respectively, SIRT1, HSP70 and SOD2 correlated positively with age. Some correlations between expression levels of particular protective proteins SIRT1, HSP70 and SOD2 were observed in the study population.ConclusionsThe increased expression of cellular protective proteins SIRT1, HSP70 and SOD2 in NK cells of the oldest seniors seems to correspond to longevity and the observed correlations may suggest the involvement of these proteins in establishing NK cell homeostasis specific for healthy ageing process.

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Section: Discussionmentioning

confidence: 99%

Expression of cellular protective proteins SIRT1, HSP70 and SOD2 correlates with age and is significantly higher in NK cells of the oldest seniors

et al. 2017

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“…It is difficult to explain why differences were not evident in previous studies, particularly when diabetic cats were considerably older and had higher body weight . Aging and obesity in humans are associated with increased oxidative stress of erythrocytes, and thus differences would have been expected in older and heavier cats …”

Section: Discussionmentioning

confidence: 89%

“…18,19 Aging and obesity in humans are associated with increased oxidative stress of erythrocytes, and thus differences would have been expected in older and heavier cats. 29,30 The median concentration of membrane carbonyls in diabetic cats at the time of diagnosis was approximately 6-fold higher than in controls, but the variability of the results was also striking. In fact, the concentration of membrane carbonyls was almost undetectable in approximately 20% of cats with DM.…”

Section: Discussionmentioning

confidence: 94%

“…However, if there had been no abnormalities in glutathione concentration, other thiol‐based antioxidants should have been decreased to explain their reduction in the present study. In fact, the thiols contain various substances with sulfhydryl groups (ie, SH), which are all targets for reactive oxygen and nitrogen species, thus counteracting the effect of pro‐oxidants . In contrast to erythrocyte cytoplasm, the concentration of thiols in erythrocyte membranes did not differ between the 2 groups.…”

Section: Discussionmentioning

confidence: 99%

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Oxidative status of erythrocytes, hyperglycemia, and hyperlipidemia in diabetic cats

Zini

Gabai

Salesov

et al. 2020

Veterinary Internal Medicne

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Background Erythrocytes of diabetic cats have decreased superoxide dismutase activity, possibly indicative of oxidative stress. Hypothesis Erythrocytes of diabetic cats undergo oxidative stress, which is caused by hyperglycemia and hyperlipidemia, and improves with treatment. Animals Twenty‐seven client‐owned cats with diabetes mellitus, 11 matched healthy cats, and 21 purpose‐bred healthy cats. Methods Prospective study. Advanced oxidized protein products, carbonyls (protein oxidation by‐products), and thiols (antioxidants) were quantified in erythrocyte membrane, thiobarbituric acid reactive substances (TBAR, lipid peroxidation by‐products), and thiols in erythrocyte cytoplasm of all cats. Comparison were performed between diabetic and matched healthy cats, between diabetic cats achieving remission or not, and among purpose‐bred cats after 10 days of hyperglycemia (n = 5) or hyperlipidemia (n = 6) versus controls treated with saline (n = 5) or untreated (n = 5). Results Compared with controls, erythrocytes of diabetic cats initially had higher median membrane carbonyls (4.6 nmol/mg total protein [range: 0.1‐37.7] versus 0.7 [0.1‐4.7], P < .001) and lower cytoplasmic TBAR (1.9 nmol/mg [0.5‐2.4] versus 2.4 [1.4‐3.5] P < .001), and thiols (419 nmol/mg [165‐621] versus 633 [353‐824], P < 0.001). After 12‐16 weeks of treatment in diabetic cats, carbonyls decreased by 13% (P < .001), but remained higher (P < .001) and TBAR and thiols lower (P = .02, P < .001) than those in controls. No differences were observed between diabetic cats achieving remission or not, and among purpose‐bred cats. Conclusions and Clinical Importance Diabetes mellitus is associated with increased protein oxidation and reduced antioxidant defenses, which persist during treatment and remission, although mild improvement in protein oxidation occurs. Short‐term hyperglycemia or hyperlipidemia does not cause oxidative stress. The reason for decreased TBAR remains unknown.

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“…Although erythrocyte has its own pathway, both enzymatic and nonenzymatic, acting as an antioxidative mechanism to protect and counteract those generated ROS [2], the imbalance production of ROS and antioxidant on erythrocyte causes a number of cardiovascular diseases such as acute coronary syndrome [3], hypercoagulation [4], endothelial cell dysfunction [5], and diabetic induced cardiovascular defects [6]. Several studies have suggested that fruit, vegetables, and cereals together with herbs may alleviate or prevent oxidative stress on erythrocyte [7–10].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Protective Effect of Phikud Navakot Extraction on Erythrocyte

Kengkoom

Ampawong

2016

Evidence-Based Complementary and Alternative Medicine

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Phikud Navakot (PN), Thai herbal remedy in National List of Essential Medicines, has been claimed to reduce many cardiovascular symptoms especially dizziness and fainting. Apart from blood supply, erythrocyte morphology, in both shape and size, is one of the main consideration factors in cardiovascular diseases and may be affected by vascular oxidative stress. However, little is known about antioxidative property of PN on erythrocyte to preserve red blood cell integrity. In this study, 1,000 μM hydrogen peroxide-induced oxidative stress was conducted on sheep erythrocyte. Three doses of PN (1, 0.5, and 0.25 mg/mL) and 10 μM of ascorbic acid were compared. The released hemoglobin absorbance was measured to demonstrate hemolysis. Electron microscopic and immunohistochemical studies were also performed to characterize dysmorphic erythrocyte and osmotic ability in relation to aquaporin- (AQP-) 1 expression, respectively. The results revealed that all doses of PN and ascorbic acid decreased the severity of dysmorphic erythrocyte, particularly echinocyte, acanthocyte, knizocyte, codocyte, clumping, and other malformations. However, the most effective was 0.5 mg/mL PN dosage. In addition, hydrostatic pressure may be increased in dysmorphic erythrocyte in association with AQP-1 upregulation. Our results demonstrated that PN composes antioxidative effect to maintain the integrity and osmotic ability on sheep erythrocyte.

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Biomarkers of oxidative stress in erythrocytes as a function of human age

Cited by 88 publications

References 61 publications

Expression of cellular protective proteins SIRT1, HSP70 and SOD2 correlates with age and is significantly higher in NK cells of the oldest seniors

Expression of cellular protective proteins SIRT1, HSP70 and SOD2 correlates with age and is significantly higher in NK cells of the oldest seniors

Oxidative status of erythrocytes, hyperglycemia, and hyperlipidemia in diabetic cats

In Vitro Protective Effect of Phikud Navakot Extraction on Erythrocyte

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