Biomarkers of Oxidative Stress: Benefits and Drawbacks for their Application in Biomonitoring of Aquatic Environments

Monserrat, José M.; Letts, Rafaela Elias; Ferreira, Josencler L. Ribas; Ventura‐Lima, Juliane; Amado, Lílian Lund; Rocha, Ângela Sanches; Gorbi, Stefania; Bocchetti, Raffaella; Benedetti, Maura; Regoli, Francesco

doi:10.1002/9781444345988.ch23

Cited by 5 publications

(7 citation statements)

References 91 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most GO categories for enriched biological processes of upregulated genes fell into the broader category of translation on both days 21 and 28, with additional enriched categories that included cellular response to retinoic acid, cellular response to vitamin D and metabolic processes. Retinoic acid is formed through the oxidation of retinol (vitamin A) and both vitamin A and D can act as nonenzymatic quenchers of ROS (Monserrat et al., 2011). In line with this, enriched GO categories for upregulated molecular functions at both time points included glutathione transferase activity, oxidoreductase activity, and peroxidase activity (Figure ).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Gene regulation underpinning increased thermal tolerance in a laboratory‐evolved coral photosymbiont

Chakravarti

Buerger

Levin³

et al. 2020

Molecular Ecology

View full text Add to dashboard Cite

Small increases in ocean temperature can disrupt the obligate symbiosis between corals and dinoflagellate microalgae, resulting in coral bleaching. Little is known about the genes that drive the physiological and bleaching response of algal symbionts to elevated temperature. Moreover, many studies to‐date have compared highly divergent strains, making it challenging to accredit specific genes to contrasting traits. Here, we compare transcriptional responses at ambient (27°C) and bleaching‐relevant (31°C) temperatures in a monoclonal, wild‐type (WT) strain of Symbiodiniaceae to those of a selected‐strain (SS), derived from the same monoclonal culture and experimentally evolved to elevated temperature over 80 generations (2.5 years). Thousands of genes were differentially expressed at a log fold‐change of >8 between the WT and SS over a 35 days temperature treatment period. At 31°C, WT cells exhibited a temporally unstable transcriptomic response upregulating genes involved in the universal stress response such as molecular chaperoning, protein repair, protein degradation and DNA repair. Comparatively, SS cells exhibited a temporally stable transcriptomic response and downregulated many stress response genes that were upregulated by the WT. Among the most highly upregulated genes in the SS at 31°C were algal transcription factors and a gene probably of bacterial origin that encodes a type II secretion system protein, suggesting interactions with bacteria may contribute to the increased thermal tolerance of the SS. Genes and functional pathways conferring thermal tolerance in the SS could be targeted in future genetic engineering experiments designed to develop thermally resilient algal symbionts for use in coral restoration and conservation.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Neither genes were significantly upregulated by either the WT@31 or SS@31. Antioxidants such as these are common biomarkers of oxidative stress (Monserrat et al., 2011). However, extracellular ROS levels were not elevated by the SS@27 in Chakravarti et al.…”

Section: Resultsmentioning

confidence: 99%

Gene regulation underpinning increased thermal tolerance in a laboratory‐evolved coral photosymbiont

Chakravarti

Buerger

Levin³

et al. 2020

Molecular Ecology

View full text Add to dashboard Cite

show abstract

“…genes and proteins involved in the OxSR) and the direction of change (i.e. upregulation or suppression) vary among species, habitats and exposure conditions5960. This may reflect the molecular complexity and redundancy of the antioxidant protection systems in the cell, as well as the dual role of ROS as important signaling molecules, on one hand, and potentially damaging agents, on the other61.…”

Section: Discussionmentioning

confidence: 99%

Long-Term Acclimation to Different Thermal Regimes Affects Molecular Responses to Heat Stress in a Freshwater Clam Corbicula Fluminea

Falfushynska

Phan

Sokolova

2016

Sci Rep

View full text Add to dashboard Cite

Global climate change (GCC) can negatively affect freshwater ecosystems. However, the degree to which freshwater populations can acclimate to long-term warming and the underlying molecular mechanisms are not yet fully understood. We used the cooling water discharge (CWD) area of a power plant as a model for long-term warming. Survival and molecular stress responses (expression of molecular chaperones, antioxidants, bioenergetic and protein synthesis biomarkers) to experimental warming (20–41 °C, +1.5 °C per day) were assessed in invasive clams Corbicula fluminea from two pristine populations and a CWD population. CWD clams had considerably higher (by ~8–12 °C) lethal temperature thresholds than clams from the pristine areas. High thermal tolerance of CWD clams was associated with overexpression of heat shock proteins HSP70, HSP90 and HSP60 and activation of protein synthesis at 38 °C. Heat shock response was prioritized over the oxidative stress response resulting in accumulation of oxidative lesions and ubiquitinated proteins during heat stress in CWD clams. Future studies should determine whether the increase in thermal tolerance in CWD clams are due to genetic adaptation and/or phenotypic plasticity. Overall, our findings indicate that C. fluminea has potential to survive and increase its invasive range during warming such as expected during GCC.

show abstract

“…Imbalance between production of ROS and scavenging processes is commonly caused by most forms of pollution stress and adverse water quality parameters. Aquatic organisms have both enzymatic and non-enzymatic antioxidant defense systems for dealing with increased production of ROS; ROS-scavenging enzymes include superoxide dismutase, catalase, glutathione peroxidase, among others, while glutathione is the main non-enzymatic antioxidant (Lesser, 2011;Monserrat et al, 2011). Therefore, both the modulation of antioxidant enzymes and changes in glutathione pool are frequently used as useful biomarkers for monitoring effects of environmental stressors on aquatic organisms (Livingstone, 2001).…”

Section: Introductionmentioning

confidence: 99%

“…The reaction of ROS with lipids is considered one of the most prevalent mechanisms of cellular injury, and malondialdehyde is a commonly measured endproduct of lipid peroxidation (Li et al, 2011;Shaw et al, 2012). Measurement of antioxidant enzymatic activities and concentrations of non-enzymatic antioxidants, as well as evaluation of products of oxidative damage are broadly employed as oxidative stress measures and have been successfully employed to characterize impact of environmental stressors in laboratory and field studies (Lesser, 2011;Monserrat et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Effects of prolonged exposure to low pH on enzymatic and non-enzymatic antioxidants in Japanese Medaka (Oryzias latipes)

Ramírez-Duarte

Jin

Kurobe

2016

Science of The Total Environment

View full text Add to dashboard Cite

Biomarkers of Oxidative Stress: Benefits and Drawbacks for their Application in Biomonitoring of Aquatic Environments

Cited by 5 publications

References 91 publications

Gene regulation underpinning increased thermal tolerance in a laboratory‐evolved coral photosymbiont

Gene regulation underpinning increased thermal tolerance in a laboratory‐evolved coral photosymbiont

Long-Term Acclimation to Different Thermal Regimes Affects Molecular Responses to Heat Stress in a Freshwater Clam Corbicula Fluminea

Effects of prolonged exposure to low pH on enzymatic and non-enzymatic antioxidants in Japanese Medaka (Oryzias latipes)

Contact Info

Product

Resources

About