Biomarkers of oxidative stress and inflammation in people witha physical disability treated with a standardized extract ofNasturtium officinale: A randomized, double‐blind, and placebo‐controlled trial

Abstract

It is well established that plants from the Brassicaceae family, particularly watercress, have been associated to reduce oxidative DNA damage. Nasturtium officinale R. Br (watercress) contains glucosinolates, with anti-inflammatory action and protective effect on human health against oxidative stress. We aimed to evaluate whether the standardized extract of Nasturtium officinale (SENO) is capable of changing biomarkers of oxidative stress and inflammation in people with physical disabilities. 65 people enrolled this study: as a control group composed by; 15 people with no physical disability assessed once, 25 people with physical disabilities using 750 mg/kg/day of SENO, and 25 people with physical disabilities using 750 mg/kg/day of placebo-control for 5 weeks. Biomarkers of oxidative stress and inflammation were analyzed on day 0 and 36. The results indicated that SENO was associated with decreasing levels of lipid peroxidation, protein carbonyl, catalase, superoxide dismutase, and C-reactive protein. Furthermore, the cytokine kit demonstrated below and out of invertible range, which was impossible to detect the inflammatory process. Despite the cytokine kit was not able to detect the inflammation; these data might provide supportive evidence that SENO, have affected positively people with physical disabilities decreasing their biomarkers of oxidative stress and C-reactive protein. Further studies are required.

Orientin Prolongs the Longevity of Caenorhabditis elegans and Postpones the Development of Neurodegenerative Diseases via Nutrition Sensing and Cellular Protective Pathways

“…Our findings support that orientin has multiple therapeutic effects, such as antioxidant, antiaging, antiviral, antiinflammation, antiadipogenesis, cardioprotective, radioprotective, and neuroprotective [5]. Although orientin was water-soluble [74], pharmacokinetic studies have proven that orientin can be rapidly absorbed and transported to various organs and tissues in the body, with better absorption, faster clearance, and no accumulation of poisoning [75]. Therefore, in the future research, its pharmacological action mechanism should be studied in depth, including experiments in mammal animal models of aging and aging-related diseases for the beneficial effects of orientin in view of the translation into clinics.…”

Effect of watercress extract supplementation on lipid profile and oxidative stress markers in overweight people with physical disability: A randomized, double‐blind, and placebo‐controlled trial

“…This may be related to the action of isothiocyanates derived from glucosinolates that accumulate in these vegetables (Armah et al, 2015). Our research shows that SENO had the positive effect of decreasing the biomarkers of oxidative stress and C‐reactive protein (CR‐P) levels in humans (Clemente et al, 2020). CR‐P is an acute inflammatory marker (Sproston & Ashworth, 2018) and has been associated with hyperlipidemia (Ma et al, 2016).…”

“…The plant material and the extraction of SENO for this study were done as described by Clemente et al (2019) in an acute and sub‐acute oral toxicity study of SENO in Wistar rats. The high‐performance liquid chromatography (HPLC) analysis and the chemical constituents of the fluid extract of Nasturtium officinale were already demonstrated by Clemente et al (2020). The placebo and the final extract (Hydro‐glycolic extract) with 5.0 mg/ml −1 of phenylethyl glucosinolate as the major marker (Anvisa, 2011; Carvalho, 2008) were used in the preclinical study (Clemente et al, 2019).…”

“…The placebo and the final extract (Hydro‐glycolic extract) with 5.0 mg/ml −1 of phenylethyl glucosinolate as the major marker (Anvisa, 2011; Carvalho, 2008) were used in the preclinical study (Clemente et al, 2019). In a recently published clinical trial (Clemente et al, 2020), it was demonstrated that up to 1,000 mg/kg/d could be the threshold for avoiding adverse effects.…”

“…The present study used the same protocol with minor modification and experimental design of a recently published paper from our laboratory (Clemente et al, 2020) but with different inclusion and exclusion criteria to select the subjects. Seventy‐five individuals with a physical disability were invited to participate in this study.…”