Biomarkers of milk fat and the risk of myocardial infarction in men and women: a prospective, matched case-control study

Warensjö, Eva; Jansson, Jan-Ha°kan; Cederholm, Tommy; Boman, Kurt; Eliasson, Mats; Hallmans, Go¨ran; Johansson, Ingegerd; Sjögren, Per

doi:10.3945/ajcn.2009.29054

Cited by 124 publications

(105 citation statements)

References 45 publications

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“…Our finding may resemble the results from a reported longer 6-month intervention with three to five daily portions of dairy foods that doubled the intake of milk, cheese and yoghurt but failed to affect any of the inflammatory markers IL-6, CRP, E-selectin or TNF-a (Wennersberg et al, 2009). Nevertheless, our data do not shed light on a recent Swedish report of a lower association between incident myocardial infarction and consumption of fermented dairy foods than when nonfermented dairy foods were eaten (Warrensjo et al, 2010).…”

Section: Discussioncontrasting

confidence: 80%

“…Individual dairy fats have not been specifically tested, which may be important given the findings in a large prospective study, suggesting a cardioprotective effect from consuming fermented but not non-fermented dairy foods (Warrensjo et al, 2010). Cheese intake had been previously found not to be associated with cardiovascular disease in observation studies (Nestel, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Circulating inflammatory and atherogenic biomarkers are not increased following single meals of dairy foods

et al. 2011

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Background/Objectives: Inflammation characterizes obesity and is nutritionally modifiable. The hypothesis of this study is that full-fat dairy foods influence circulating inflammatory and atherogenic biomarkers according to fermentation status. Subjects/Methods: Thirteen overweight subjects participated in five test meals. Single breakfasts containing control low-fat milk or 45 g fat from butter, cream, yoghurt or cheese were tested over 3 weeks. Plasmas obtained 3 and 6 h were later analyzed for inflammatory markers interleukin (IL)-6, IL-1b, tumor necrosis factor-a and high-sensitive C-reactive protein, and atherogenesis-related markers monocyte chemoattractant protein-1, macrophage inflammatory protein-1a, intercellular adhesion molecule-1 and vascular cell adhesion molecule-1. A 4-week study in 12 subjects compared the effects on these biomarkers of diets containing B50 g dairy fat daily as either butter, cream and ice cream (non-fermented) or cheese plus yoghurt (fermented) dairy foods. Results: In single-meal study, one outlier subject showed marked increments in biomarkers, hence the following results apply to 12. Within group analysis includes significant falls at 3 h in four inflammatory markers after cream, butter and low fat, and three atherogenesis-related biomarkers after cream. Changes were few after cheese and yoghurt. By 6 h, most values returned to baseline. However, between group analysis showed no differences between the five meals. The 4-week study showed no significant differences in fasting biomarker concentrations between non-fermented and fermented dairy diets. Conclusions: Single high-fat meals containing sequentially four different full-fat dairy foods did not increase eight circulating biomarkers related to inflammation or atherogenesis. Among subjects, significant falls occurred at 3 h in inflammatory biomarkers after cream and butter but were not specific for full-fat dairy foods. We could not confirm the reported increments in inflammation after fat meals.

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Section: Discussioncontrasting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Circulating inflammatory and atherogenic biomarkers are not increased following single meals of dairy foods

et al. 2011

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“…Several circulating fatty acids (e.g., 15:0, 17:0) have been suggested as biomarkers of dairy consumption (17)(18)(19), and they were reported to be associated with lower risk of diabetes (20,21) or cardiovascular disease (22,23). Previously, we found that erythrocyte trans-18:1 isomers are associated with dairy product consumption and lower prevalence of metabolic syndrome and diabetes in middle-aged and older Chinese men and women (24).…”

mentioning

confidence: 71%

Dairy Consumption, Type 2 Diabetes, and Changes in Cardiometabolic Traits: A Prospective Cohort Study of Middle-Aged and Older Chinese in Beijing and Shanghai

Zong

Sun

et al. 2013

Diabetes Care

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OBJECTIVETo prospectively investigate associations of dairy consumption with risk of type 2 diabetes and changes of cardiometabolic traits. RESEARCH DESIGN AND METHODSIn 2005, 2,091 middle-aged and older Chinese men and women were recruited and followed for 6 years. Baseline dairy consumption was assessed by a 74-item food frequency questionnaire. Erythrocyte fatty acids were analyzed by gas chromatography coupled with flame ion detector. Cardiometabolic traits were measured at both baseline and follow-up visits. RESULTSOnly 1,202 (57.5%) participants reported any dairy consumption, with a median intake of 0.89 (interquartile range 0.19-1.03) serving/day. Compared with nonconsumers, the relative risks (RRs) of type 2 diabetes among those having 0.5-1 serving/day and >1 serving/day were 0.70 (95% CI 0.55-0.88) and 0.65 (0.49-0.85), respectively, after multivariate adjustment (P trend < 0.001), which were attenuated by further adjusting for changes in glucose during follow-up (P trend = 0.07). Total dairy consumption was associated with favorable changes in glucose, waist circumference, BMI, diastolic blood pressure (all P trend < 0.05), and systolic blood pressure (P trend = 0.05) after multivariate adjustment, including baseline values of dependent variables. Erythrocyte trans-18:1 isomers were significantly correlated with total dairy consumption (r s = 0.37, P trend < 0.001), and these dairy food biomarkers were associated with a lower risk of type 2 diabetes. The RR of type 2 diabetes comparing extreme quartiles of trans-18:1 isomers was 0.82 (0.65-1.04, P trend = 0.02), which was attenuated after adjustment for dairy consumption (P trend = 0.15). CONCLUSIONSDairy consumption was associated with a significantly lower risk of type 2 diabetes and favorable changes of cardiometabolic traits in Chinese.

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“…Milk is an important biological fluid that can be easily obtained and used for biomarker identification studies (Saleh et al, 1998;Warensjö et al, 2010;Klein et al, 2012). Milk is also an abundant source of dietary miRNAs (Baier et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Comparative studies of two methods for miRNA isolation from milk whey

Jin

Wei

Liu

et al. 2015

J. Zhejiang Univ. Sci. B

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MicroRNAs (miRNAs) from milk whey have been considered for their potential as noninvasive biomarkers for milk quality control and disease diagnosis. However, standard protocols for miRNA isolation and quantification from milk whey are not well established. The objective of this study was to compare two methods for the isolation of miRNAs from milk whey. These two methods were modified phenol-based technique (Trizol LS ® followed by phenol precipitation, the TP method) and combined phenol and column-based approach (Trizol LS ® followed by cleanup using the miRNeasy kit, the TM method). Yield and quality of RNA were rigorously measured using a NanoDrop ND-1000 spectrophotometer and then the distribution of RNA was precisely detected in a Bioanalyzer 2100 instrument by microchip gel electrophoresis. Several endogenous miRNAs (bta-miR-141, bta-miR-146a, bta-miR-148a, bta-miR-200c, bta-miR-362, and bta-miR-375) and an exogenous spike-in synthetic control miRNA (cel-miR-39) were quantified by real-time polymerase chain reaction (PCR) to examine the apparent recovery efficiency of milk whey miRNAs. Both methods could successfully isolate sufficient small RNA (<200 nt) from milk whey, and their yields were quite similar. However, the quantification results show that the total miRNA recovery efficiency by the TM method is superior to that by the TP method. The TM method performed better than the TP for recovery of milk whey miRNA due to its consistency and good repeatability in endogenous and spike-in miRNA recovery. Additionally, quantitative recovery analysis of a spike-in miRNA may be more accurate to reflect the milk whey miRNA recovery efficiency than using traditional RNA quality analysis instruments (NanoDrop or Bioanalyzer 2100).

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Biomarkers of milk fat and the risk of myocardial infarction in men and women: a prospective, matched case-control study

Abstract: Milk fat biomarkers were associated with a lower risk of developing a first MI, especially in women. This was partly confirmed in analysis of fermented milk and cheese intake. Components of metabolic syndrome were observed as potential intermediates for the risk relations.

Cited by 124 publications

References 45 publications

Circulating inflammatory and atherogenic biomarkers are not increased following single meals of dairy foods

Circulating inflammatory and atherogenic biomarkers are not increased following single meals of dairy foods

Dairy Consumption, Type 2 Diabetes, and Changes in Cardiometabolic Traits: A Prospective Cohort Study of Middle-Aged and Older Chinese in Beijing and Shanghai

Comparative studies of two methods for miRNA isolation from milk whey

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