Biomarkers of in vivo fertility in sperm and seminal plasma of fertile stallions

Novak, Susan; Smith, Taylor A.; Paradis, François; Burwash, Les; Dyck, Michael K.; Foxcroft, G. R.; Dixon, Walter T.

doi:10.1016/j.theriogenology.2010.04.025

Cited by 116 publications

(92 citation statements)

References 22 publications

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“…In the present study we chose six stallions with differing fertility, as evaluated in terms of semen profile (progressive motility and percentage of spermatozoa with normal morphology) and percentage of pregnancies per season, calculated by dividing the number of all pregnant mares (for each stallion individually) by the number of mares bred during the year. Recently, many developments have been achieved in the field of gene expression analysis, providing new molecular detection tools to aid our understanding of stallion fertility (Bright et al 2009;Novak et al 2010). RNA in the spermatozoa could be used in genomic analysis to evaluate male fertility.…”

Section: Discussionmentioning

confidence: 99%

Implication of transcriptome profiling of spermatozoa for stallion fertility

Suliman

Becker

Wimmers

2018

Reprod. Fertil. Dev.

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Abstract. Poor fertility of breeding stallions is a recognised problem in the equine industry. The aim of the present study was to detect molecular pathways using two groups of stallions that differed in pregnancy rates as well as in the proportion of normal and motile spermatozoa. RNA was isolated from spermatozoa of each stallion and microarray data were analysed to obtain a list of genes for which transcript abundance differed between the groups (P #0.05, fold change $1.2). In all, there were 437 differentially expressed (DE) genes between the two groups (P # 0.05, fold change $1.2). Next, the DE genes were analysed using Database for Annotation, Visualisation, and Integrated Discovery (DAVID). Finally, ingenuity pathways analysis (IPA) was used to identify top biological functions and significant canonical pathways associated with the DE genes. Analysis using the DAVID database showed significant enrichment in the gene ontology (GO) term 'RNA binding' (P ¼ 0.05) and in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway cytokinecytokine receptor interaction (P ¼ 0.02). Furthermore, IPA analysis showed interconnected biological functions and canonical pathways involved in the regulation of spermatogenesis and male fertility. In addition, significantly enriched metabolic pathways were identified. In conclusion, the present study has identified, for the first time, molecular processes in stallion spermatozoa that could be associated with stallion fertility.

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Section: Discussionmentioning

confidence: 99%

Implication of transcriptome profiling of spermatozoa for stallion fertility

Suliman

Becker

Wimmers

2018

Reprod. Fertil. Dev.

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“…Thacker et al [83] observed overexpression of this protein in sperm from fertile men. CLU was suggested as a fertility biomarker in bulls [95], stallions [97], and humans [40]. Taken together, these results suggest that protein ubiquitination might be altered in the High sperm DNA fragmentation group, leading to defective removal of sperm with damaged DNA and incomplete response to unfolded proteins.…”

Section: Enriched Functions In the Low Sperm Dna Fragmentation Groupmentioning

confidence: 91%

Unraveling the sperm proteome and post-genomic pathways associated with sperm nuclear DNA fragmentation

Intasqui

Camargo

Giudice

et al. 2013

J Assist Reprod Genet

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Purpose Sperm DNA fragmentation has been suggested as a marker for infertility diagnosis and prognosis. Hence, understanding its impact on male physiology and post-genomic pathways would be clinically important. We performed the proteomics and functional enrichment analyses of viable spermatozoa from ejaculates with low and high sperm DNA fragmentation to identify protein expression and pathways altered in association with sperm DNA fragmentation. Methods Sperm DNA fragmentation using the Comet assay and the Komet 6.0.1 software was assessed in raw samples from 89 subjects from a human reproduction service. The Low and High sperm DNA fragmentation groups were formed according to the Olive Tail Moment variable. Spermatozoa proteins from these groups were pooled and analyzed by a shotgun proteomic approach (2D nanoUPLC-ESI-MS E ). Differentially expressed proteins were used for a functional enrichment study. Results Two hundred and fifty-seven proteins were identified or quantified in sperm from the Low and High sperm DNA fragmentation groups. Of these, seventy-one proteins were exclusively or overexpressed in the Low group, whereas twenty-three proteins were exclusively or overexpressed in the High group. One hundred and sixty-three proteins were conserved between these groups. We also functionally related the differentially expressed proteins in viable spermatozoa from the groups. Processes such as triacylglycerol metabolism, energy production, protein folding, response to unfolded proteins, and cellular detoxification were found to be altered in these cells. Conclusions Sperm DNA fragmentation is associated with differential protein expression in viable spermatozoa. These proteins may potentially be used as biomarkers for sperm DNA integrity.

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“…21 Moreover, alpha-enolase was identified as having a positive relationship with the first cycle conception rate in stallions. 22 In infertile men, sperm-specific enolase enzyme activity was elevated in normal sperm compared to abnormal sperm. 23 All these experimental evidence demonstrate how alpha-enolase expression could be linked with sperm motility and healthiness, and in this way could influence the ERCR rate.…”

Section: View Article Onlinementioning

confidence: 98%

Unravelling the bull fertility proteome

et al. 2013

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In the last few decades a negative association between the level of milk production and fertility has been observed. Currently, the most utilized method of measuring male fertility employed by the livestock industry is related to the Non-Return Rate (NRR). Through differential proteome analysis, this study evaluated changes in the expression of the protein profile of spermatozoa collected from 16 bulls with different levels of field fertility expressed as an estimated relative conception rate (ERCR). The main aim is to identify putative protein markers to be used as putative indices of fertility. Two dimensional electrophoresis coupled with mass spectrometry analysis was used for protein separation and identification. To improve differential proteome analysis among experimental groups, a part of shotgun MS analysis was also performed. Three protein spots showed a differential expression pattern among all ERCR classes. Alpha enolase was significantly down-regulated in the ERCRÀ group, while two other proteins, isocitrate dehydrogenase and triosephosphate isomerase, were up-regulated in ERCRÀ in comparison to ERCR+. Alpha-enolase and isocitrate dehydrogenase subunit alpha (IDH-alpha) have been described in the literature for having a potential role in bull fertility. The possibility of determining protein biomarkers for fertility is more useful and less expensive than ERCR for acquiring rapid estimation of fertility because it does not require the use of field insemination trials. Shotgun MS analysis conducted on the same samples revealed 7 proteins down-regulated in the ERCRÀ group and 1 protein up-regulated. Among these proteins, calmodulin, ATP synthase mitochondrial subunits alpha and delta, malate dehydrogenase and sperm equatorial segment protein 1 were shown to be linked with sperm fertility.

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Biomarkers of in vivo fertility in sperm and seminal plasma of fertile stallions

Cited by 116 publications

References 22 publications

Implication of transcriptome profiling of spermatozoa for stallion fertility

Implication of transcriptome profiling of spermatozoa for stallion fertility

Unraveling the sperm proteome and post-genomic pathways associated with sperm nuclear DNA fragmentation

Unravelling the bull fertility proteome

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