Biomarkers of caspofungin resistance in <i>Candida albicans</i> isolates: A proteomic approach

Cesare, Giuseppe Buda De; Hafez, Ahmed Ibrahem; Stead, David; Lloréns, Carlos; Munro, Carol A.

doi:10.1080/21505594.2022.2081291

Cited by 6 publications

(7 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The pH-responsive protein 2 (PHR2), also known as 1,3-beta-glucanosyltransferase, is an important component of the cell wall architecture. In C. albicans , PHR2 had an increased expression level after caspofungin treatment, and this protein was also detected in high levels in caspofungin-resistant isolates [ 40 ]. Our result reinforces the relevant role of PHR2 in responding to caspofungin in distinct Candida species.…”

Section: Discussionmentioning

confidence: 99%

Caspofungin Affects Extracellular Vesicle Production and Cargo in Candida auris

Amatuzzi

Zamith-Miranda

Rocha

et al. 2022

JoF

View full text Add to dashboard Cite

Antifungal resistance has become more frequent, either due to the emergence of naturally resistant species or the development of mechanisms that lead to resistance in previously susceptible species. Among these fungal species of global threat, Candida auris stands out for commonly being highly resistant to antifungal drugs, and some isolates are pan-resistant. The rate of mortality linked to C. auris infections varies from 28% to 78%. In this study, we characterized C. auris extracellular vesicles (EVs) in the presence of caspofungin, an echinocandin, which is the recommended first line antifungal for the treatment of infections due to this emerging pathogen. Furthermore, we also analyzed the protein and RNA content of EVs generated by C. auris cultivated with or without treatment with caspofungin. We observed that caspofungin led to the increased production of EVs, and treatment also altered the type and quantity of RNA molecules and proteins enclosed in the EVs. There were distinct classes of RNAs in the EVs with ncRNAs being the most identified molecules, and tRNA-fragments (tRFs) were abundant in each of the strains studied. We also identified anti-sense RNAs, varying from 21 to 55 nt in length. The differentially abundant mRNAs detected in EVs isolated from yeast subjected to caspofungin treatment were related to translation, nucleosome core and cell wall. The differentially regulated proteins identified in the EVs produced during caspofungin treatment were consistent with the results observed with the RNAs, with the enriched terms being related to translation and cell wall. Our study adds new information on how an echinocandin can affect the EV pathway, which is associated with the yeast cell being able to evade treatment and persist in the host. The ability of C. auris to efficiently alter the composition of EVs may represent a mechanism for the fungus to mitigate the effects of antifungal agents.

show abstract

Section: Discussionmentioning

confidence: 99%

Caspofungin Affects Extracellular Vesicle Production and Cargo in Candida auris

Amatuzzi

Zamith-Miranda

Rocha

et al. 2022

JoF

View full text Add to dashboard Cite

show abstract

“…that can be transmitted from patient to patient, population level resistance may also rise and spread 81,82 . Further development and wider adoption of stewardship programmes is needed to ensure that prescribing follows evidence-based guidelines, and future research may be guided by the identification of biomarkers of drug resistance 83,84 . A body of literature attests to the fact that stewardship programmes can reduce inappropriate prescribing, and thus reduce selective pressure, without adversely affecting clinical outcomesalthough such studies may often be insufficiently powered to detect changes in clinical endpoints compared with changes in drug use or expenditure 85,86 .…”

Section: Translational Pipeline and Strategies To Reduce And Mitigate...mentioning

confidence: 99%

The importance of antimicrobial resistance in medical mycology

et al. 2022

View full text Add to dashboard Cite

Prior to the SARS-CoV-2 pandemic, antibiotic resistance was listed as the major global health care priority. Some analyses, including the O’Neill report, have predicted that deaths due to drug-resistant bacterial infections may eclipse the total number of cancer deaths by 2050. Although fungal infections remain in the shadow of public awareness, total attributable annual deaths are similar to, or exceeds, global mortalities due to malaria, tuberculosis or HIV. The impact of fungal infections has been exacerbated by the steady rise of antifungal drug resistant strains and species which reflects the widespread use of antifungals for prophylaxis and therapy, and in the case of azole resistance in Aspergillus, has been linked to the widespread agricultural use of antifungals. This review, based on a workshop hosted by the Medical Research Council and the University of Exeter, illuminates the problem of antifungal resistance and suggests how this growing threat might be mitigated.

show abstract

“…Polyene drugs can be fungicidal but are toxic to the host, while azoles are fungistatic, therefore fungi are more prone to develop resistance. 2 C. albicans colonization occurs as a result of the fungus adhering to the epithelium, which then invades the vaginal epithelial cells, causing infection 1,3 and candidiasis. 4 Candidalysin is a fungal peptide poison that is important in mucosal infections because it directly damages epithelial membranes.…”

Section: Introductionmentioning

confidence: 99%

“…Polyene drugs can be fungicidal but are toxic to the host, while azoles are fungistatic, therefore fungi are more prone to develop resistance. 2 …”

Section: Introductionmentioning

confidence: 99%

The Effects of Holothurin and Caspofungin on the Vaginal Cell Inflammation Parameters of the Rattus norvegicus Strain Post Induction of Candida albicans

Handayani¹,

Nurdiana²,

Winarsih³

et al. 2023

IDR

View full text Add to dashboard Cite

Candida albicans (C. albicans) is a fungus that causes superficial and invasive candidiasis in its host. Caspofungin, has been widely used as a synthetic antifungal, whereas holothurin has been shown to have potential as a natural antifungal. The purpose of this study was to see how holothurin and caspofungin affected the number of C. albicans's colonies, LDH levels, and the number of inflammatory cells in vagina of Rattus norvegicus. Patients and Methods: Design of this research is using posttest only with control group design with 48 Rattus norvegicus Wistar strains used in this study were divided into six treatment groups. Each group was divided into three-time intervals of 12, 24, and 48 hours. LDH markers were tested using ELISA, inflammatory cells were counted manually, and the number of colonies was calculated using colonymetry before being diluted with NaCl 0.9% and planted in sabouraud dextrose agar (SDA). Results: According to the findings, inflammatory cells in the treatment of holothurin (48-hours) had an OR of 1.68 CI (−0.79-4.16) P = 0.09 and caspofungin had an OR of 4.18 CI (1.26-9.63) P = 0.09. Meanwhile, LDH in the holothurin (48-hour) treatment obtained OR 348, CI (286-410), P=0.03, and Caspofungin OR 393, CI (277-508), P=0.03. Colonies were obtained with zero numbers in the holothurin treatment (48 hours) and with Caspofungin OR 393, CI (273-508) P=0.00. Conclusion: Holothurin and caspofungin administration reduced the number of C. albicans colonies and the number of inflammatory cells (P 0.05), implying that holothurin and caspofungin could prevent C. albicans infection.

show abstract

Biomarkers of caspofungin resistance in Candida albicans isolates: A proteomic approach

Cited by 6 publications

References 56 publications

Caspofungin Affects Extracellular Vesicle Production and Cargo in Candida auris

Caspofungin Affects Extracellular Vesicle Production and Cargo in Candida auris

The importance of antimicrobial resistance in medical mycology

The Effects of Holothurin and Caspofungin on the Vaginal Cell Inflammation Parameters of the Rattus norvegicus Strain Post Induction of Candida albicans

Contact Info

Product

Resources

About