Biomarkers of bacterial spore germination

Tehri, Nimisha; Kumar, Naresh; Raghu, H. V.; Vashishth, Amit

doi:10.1007/s13213-018-1361-z

Cited by 22 publications

(13 citation statements)

References 71 publications

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“…[30][31][32][33][34] Bacterial spores are robust, vegetative, and metabolically dormant, and are produced to survive the severe and adverse climatic conditions of starvation and stress. 132,133 Exposure to germinants induces the germination of spores, which are highly resistant to varying ranges of temperature, pressure, desiccation, ultraviolet radiation, pH extremes, and noxious chemicals, including hypochlorite, aldehydes, ethylene oxide, and several other extreme conditions. 132,134 In the presence of specific small molecule germinants such as L-Ala or other nutrients, spores interact via germinant-specific receptors to reactivate their metabolism and allow for vegetative growth in Bacillus spp.…”

Section: Prokaryote Production Of D-aasmentioning

confidence: 99%

Promising applications of D-amino acids in periprosthetic joint infection

et al. 2023

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Due to the rise in our aging population, a disproportionate demand for total joint arthroplasty (TJA) in the elderly is forecast. Periprosthetic joint infection (PJI) represents one of the most challenging complications that can occur following TJA, and as the number of primary and revision TJAs continues to rise, an increasing PJI burden is projected. Despite advances in operating room sterility, antiseptic protocols, and surgical techniques, approaches to prevent and treat PJI remain difficult, primarily due to the formation of microbial biofilms. This difficulty motivates researchers to continue searching for an effective antimicrobial strategy. The dextrorotatory-isoforms of amino acids (D-AAs) are essential components of peptidoglycan within the bacterial cell wall, providing strength and structural integrity in a diverse range of species. Among many tasks, D-AAs regulate cell morphology, spore germination, and bacterial survival, evasion, subversion, and adhesion in the host immune system. When administered exogenously, accumulating data have demonstrated that D-AAs play a pivotal role against bacterial adhesion to abiotic surfaces and subsequent biofilm formation; furthermore, D-AAs have substantial efficacy in promoting biofilm disassembly. This presents D-AAs as promising and novel targets for future therapeutic approaches. Despite their emerging antibacterial efficacy, their role in disrupting PJI biofilm formation, the disassembly of established TJA biofilm, and the host bone tissue response remains largely unexplored. This review aims to examine the role of D-AAs in the context of TJAs. Data to date suggest that D-AA bioengineering may serve as a promising future strategy in the prevention and treatment of PJI.

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Section: Prokaryote Production Of D-aasmentioning

confidence: 99%

Promising applications of D-amino acids in periprosthetic joint infection

et al. 2023

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“…Independently from any previous biocide treatment, regrown B. cereus (from persister cells and endospores) in suspension had higher antimicrobial susceptibility than the regular counterparts (p < 0.05). Endospores from B. cereus biofilms will germinate and convert into vegetative cells when favorable environmental conditions are established [40]. The new vegetative bacteria are again active and susceptible to the antimicrobial activity of biocides [40].…”

Section: Antimicrobial Susceptibility Of Regrown Cells In Suspensionmentioning

confidence: 99%

“…Endospores from B. cereus biofilms will germinate and convert into vegetative cells when favorable environmental conditions are established [40]. The new vegetative bacteria are again active and susceptible to the antimicrobial activity of biocides [40]. No significant differences were found between the susceptibility of the regular planktonic cultures of P. fluorescens and regrown P. fluorescens in suspension (p > 0.05), which demonstrated the return to a susceptible planktonic state due to the loss of mechanisms of protection typical of biofilms [34].…”

Section: Antimicrobial Susceptibility Of Regrown Cells In Suspensionmentioning

confidence: 99%

Antimicrobial Susceptibility of Persister Biofilm Cells of Bacillus cereus and Pseudomonas fluorescens

et al. 2022

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The present study evaluates the antimicrobial susceptibility of persister cells of Bacillus cereus and Pseudomonas fluorescens after their regrowth in suspension and as biofilms. Two conventional (benzalkonium chloride—BAC and peracetic acid—PAA) and two emerging biocides (glycolic acid—GA and glyoxal—GO) were selected for this study. Persister cells resulted from biofilms subjected to a critical treatment using the selected biocides. All biocide treatments developed B. cereus persister cells, except PAA that effectively reduced the levels of vegetative cells and endospores. P. fluorescens persister cells comprise viable and viable but non-culturable cells. Afterwards, persister cells were regrown in suspension and in biofilms and were subjected to a second biocide treatment. In general, planktonic cultures of regrown persister cells in suspension lost their antimicrobial tolerance, for both bacteria. Regrown biofilms of persister cells had antimicrobial susceptibility close to those regrown biofilms of biocide-untreated cells, except for regrown biofilms of persister P. fluorescens after BAC treatment, which demonstrated increased antimicrobial tolerance. The most active biocide against persister cells was PAA, which did not promote changes in susceptibility after their regrowth. In conclusion, persister cells are ubiquitous within biofilms and survive after critical biocide treatment. The descendant planktonic and biofilms populations showed similar properties as the original ones.

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“…Pesticides are known to be a potential carcinogen and their effect on humans and their occurrence in diverse foods including dairy products and cereal-based food. Pesticide deposits in food items have been related to a wide assortment of human illnesses, extending from short to long-term harmful effects with toxigenic impact 1,2 . Due to high human health risks, there is a need to constantly monitoring the pesticide exposure or content in the food.…”

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confidence: 99%

Rapid detection of pesticide in milk, cereal and cereal based food and fruit juices using paper strip-based sensor

Dasriya

Joshi

Ranveer

et al. 2021

Sci Rep

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The study was aimed to validate paper strip sensors for the detection of pesticide residues in milk, cereal-based food, and fruit juices in comparison with GC–MS/MS under field conditions. The detection limit of pesticide using rapid paper strip sensor for organophosphate, carbamate, organochlorine, fungicide, and herbicide group ranges from 1 to 10, 1–50, 250–500, 1–50, and 1 ppb, respectively in milk and milk product, cereal-based food and fruit juices. Among 125 samples of milk samples collected from the market 33 milk samples comprising 31 raw milk and 2 pasteurized milk found positive for pesticide using the strip-based sensor. In cereal based food and fruit juice samples, 6 cereal flours and 4 fruit juices were found positive for pesticide residues. The pesticide positive samples were further evaluated quantitatively using GC–MS/MS wherein 7 samples comprised of raw milk, pasteurized milk, rice flour, wheat flour, maize flour, apple juice, and pomegranate juice have shown the presence of chlorpyrifos, chlorpyrifos-methyl, α-endosulfan, β-endosulfan DDD and DDT at trace level as well as at above MRL level. It is envisaged that the developed paper strip sensor can be a potential tool in the rapid and cost-effective screening of a large number of food samples for pesticide residues.

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Biomarkers of bacterial spore germination

Cited by 22 publications

References 71 publications

Promising applications of D-amino acids in periprosthetic joint infection

Promising applications of D-amino acids in periprosthetic joint infection

Antimicrobial Susceptibility of Persister Biofilm Cells of Bacillus cereus and Pseudomonas fluorescens

Rapid detection of pesticide in milk, cereal and cereal based food and fruit juices using paper strip-based sensor

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