Biomarkers of Alzheimer's disease and neurodegeneration in dried blood spots—A new collection method for remote settings

Huber, Hanna; Blennow, Kaj; Zetterberg, Henrik; Boada, Mercé; Jeromin, Andreas; Weninger, Haley; Nuñez‐Llaves, Raul; Aguilera, Núria; Ramis, Maribel; Simrén, Joel; Nilsson, Johanna; Lantero‐Rodriguez, Juan; Orellana, Adelina; García‐Gutiérrez, Fernando; Morató, Xavier; Ashton, Nicholas J.; Montoliu‐Gaya, Laia

doi:10.1002/alz.13697

Cited by 8 publications

(1 citation statement)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In neurological disorders, NfL is considered a very suitable biomarker as it is highly neuron-specific [ 3 , 6 ]. Further advantages of NfL are a long half-life in body fluids such as CSF and peripheral blood of about 3 to 4 weeks and pre-analytical long-term stability in stored CSF, serum and plasma samples of 7 days at room temperature, more than 10 years at −80 °C as well as in dried blood spot samples for potentially long time periods [ 18 , 19 , 20 , 21 ]. The fourth-generation single-molecule array (SiMoA) technology for NfL measurements is highly sensitive and allows exact quantification even of low NfL sample concentrations with a lower limit of 0.8 ng/L [ 3 , 22 ].…”

Section: Discussionmentioning

confidence: 99%

Neurofilament Light Chain Concentration in Cerebrospinal Fluid in Children with Acute Nontraumatic Neurological Disorders

Geis,

Gutzeit,

Disse

et al. 2024

Children

View full text Add to dashboard Cite

(1) Introduction: This pilot study aimed to analyze neurofilament light chain levels in cerebrospinal fluid (cNfL) in a cohort of children with different acute nontraumatic neurological conditions. (2) Methods: This prospective observational cohort study consisted of 35 children aged 3 months to 17 years and was performed from November 2017 to December 2019. Patients’ clinical data were reviewed, and patients were assigned to the following groups: n = 10 (28.6%) meningitis, 5 (14.3%) Bell’s palsy, 7 (20.0%) febrile non-CNS infection, 3 (8.6%) complex febrile seizure, 4 (11.4%) idiopathic intracranial hypertension, and 6 (17.1%) others. cNfL levels were measured using a sensitive single-molecule array assay. (3) Results: The cNfL levels [median (range)] in children with meningitis were 120.5 pg/mL (58.1–205.4), in Bell’s palsy 88.6 pg/mL (48.8–144.5), in febrile non-CNS infection 103.9 pg/mL (60.1–210.8), in complex febrile seizure 56 pg/mL (53.2–58.3), and in idiopathic intracranial hypertension 97.1 pg/mL (60.1–124.6). Within the meningitis group, children with Lyme neuroborreliosis (LNB) had significantly higher cNfL concentrations (median 147.9 pg/mL; range 87.8–205.4 pg/mL) than children with enterovirus meningitis (72.5 pg/mL; 58.1–95.6 pg/mL; p = 0.048) and non-significantly higher cNfL levels when compared to Bell’s palsy (88.6 pg/mL; 48.8–144.5 pg/mL; p = 0.082). There was no correlation between cNfL levels and age. (4) Conclusions: Although the number of patients in this pilot study cohort is limited, higher cNfL levels in children with LNB compared to those with viral meningitis (significant) and Bell’s palsy (trend) may indicate the potential of cNfL as a biomarker in the differential diagnosis of pediatric meningitis and facial palsy.

show abstract

Section: Discussionmentioning

confidence: 99%

Neurofilament Light Chain Concentration in Cerebrospinal Fluid in Children with Acute Nontraumatic Neurological Disorders

Geis,

Gutzeit,

Disse

et al. 2024

Children

View full text Add to dashboard Cite

show abstract

Significance of plasma p‐tau217 in predicting long‐term dementia risk in older community residents: Insights from machine learning approaches

Xiao,

Zhou,

Zhao

et al. 2024

Alzheimer's & Dementia

View full text Add to dashboard Cite

INTRODUCTIONWhether plasma biomarkers play roles in predicting incident dementia among the general population is worth exploring.METHODSA total of 1857 baseline dementia‐free older adults with follow‐ups up to 13.5 years were included from a community‐based cohort. The Recursive Feature Elimination (RFE) algorithm aided in feature selection from 90 candidate predictors to construct logistic regression, naive Bayes, bagged trees, and random forest models. Area under the curve (AUC) was used to assess the model performance for predicting incident dementia.RESULTSDuring the follow‐up of 12,716 person‐years, 207 participants developed dementia. Four predictive models, incorporated plasma p‐tau217, age, and scores of MMSE, STICK, and AVLT, exhibited AUCs ranging from 0.79 to 0.96 in testing datasets. These models maintained robustness across various subgroups and sensitivity analyses.DISCUSSIONPlasma p‐tau217 outperforms most traditional variables and may be used to preliminarily screen older individuals at high risk of dementia.Highlights Plasma p‐tau217 showed comparable importance with age and cognitive tests in predicting incident dementia among community older adults. Machine learning models combining plasma p‐tau217, age, and cognitive tests exhibited excellent performance in predicting incident dementia. The training models demonstrated robustness in subgroup and sensitivity analysis.

show abstract

Circulating apoE4 protein levels from dried blood spots predict cognitive function in a large population‐based survey setting

Deza‐Lougovski,

Weiss,

Horton

et al. 2024

Alzheimer's & Dementia

View full text Add to dashboard Cite

INTRODUCTIONThe apolipoprotein E (APOE) ε4 allele carries risk for cognitive impairment, but whether the level of circulating apoE4 protein in carriers affects cognition is unclear, as is how health and lifestyle impact circulating apoE4 levels.METHODSWe assayed apoE4 protein levels in dried blood spots of 12,532 adults aged 50+. Regression analyses tested the likelihood of cognitive impairment between groups and within those with detected apoE4 protein. Predictors of circulating apoE4 were assessed.RESULTSWe detected protein binding that indicates the presence of an APOE ε4 allele in 28.4% of this group. This group was more likely to have cognitive impairment, and this risk increases with age. However, higher apoE4 levels were associated with less likelihood of cognitive impairment within this group. Antihypertensive medication predicted apoE4 protein levels.DISCUSSIONThe apoE4 isoform is associated with a deficient protein and worse cognition. This association is modulated by the level of circulating apoE4 protein in ε4 carriers.Highlights An assay to quantify apoE4 levels from dried blood spot samples was applied. The apoE4 protein was detected as specific binding at ≥30,000 pg/mL in 28.4% of samples. Having the apoE4 protein was associated with worse cognitive performance. Higher apoE4 protein levels in those who have it were associated with better cognition. Cardiovascular factors influenced levels of apoE4 protein.

show abstract

Biomarkers of Alzheimer's disease and neurodegeneration in dried blood spots—A new collection method for remote settings

Cited by 8 publications

References 51 publications

Neurofilament Light Chain Concentration in Cerebrospinal Fluid in Children with Acute Nontraumatic Neurological Disorders

Neurofilament Light Chain Concentration in Cerebrospinal Fluid in Children with Acute Nontraumatic Neurological Disorders

Significance of plasma p‐tau217 in predicting long‐term dementia risk in older community residents: Insights from machine learning approaches

Circulating apoE4 protein levels from dried blood spots predict cognitive function in a large population‐based survey setting

Contact Info

Product

Resources

About