Biomarkers involved in the proliferation of the odontogenic keratocyst, glandular odontogenic cyst and botryoid odontogenic cyst

Felipe, Joaquim Cezar; França, Glória Maria de; Barros, Caio César Da Silva; Felix, Fernanda Aragão; Silva, Weslay Rodrigues da; Lucena, Hévio Freitas de; Oliveira, Cláudia Nunes; Galvão, Hébel Cavalcanti

doi:10.1007/s10006-021-01026-x

Cited by 7 publications

(8 citation statements)

References 38 publications

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“…The vast majority of studies (24 out of 29) reported experiments on FFPE tissue samples ( Table S3 ), two studies included both fresh-frozen and FFPE samples [ 6 , 39 ], one study reviewed only fresh-frozen samples [ 42 ], and in vitro analysis of human odontogenic lesion-derived cells was performed in two studies [ 43 , 47 ]. A minority of studies (10 out of 29) documented the microscopic diagnosis of included odontogenic entities, either by providing a detailed microscopic description [ 6 , 11 , 12 , 29 , 35 ] or by mentioning an appropriate reference [ 13 , 32 , 36 , 37 , 39 ], e.g., the WHO 2017 [ 1 ] or 2005 [ 51 ] editions for odontogenic tumors and cysts.…”

Section: Resultsmentioning

confidence: 99%

“…OCT4 was negative [ 28 ] or focally expressed in the superficial epithelial layer [ 32 ] or in a few stromal cells of OKC [ 10 ], whereas one study employing a polyclonal anti-OCT4 antibody reported 100% positivity in OKC [ 31 ]. In contrast, six studies agreed on the strong nuclear expression of the SOX2 protein in OKC epithelial cells, mainly in the basal and/or intermediate layers [ 28 , 32 , 33 , 36 , 37 , 42 ], while one study showed SOX2 stromal positivity [ 10 ]. In addition, SOX2 RNA was significantly upregulated compared with AMBL or DF, according to a microarray-based [ 42 ] and an RNA-Seq-based study [ 37 ], respectively.…”

Section: Resultsmentioning

confidence: 99%

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The Stem Cell Expression Profile of Odontogenic Tumors and Cysts: A Systematic Review and Meta-Analysis

Kalogirou,

Lekakis,

Petroulias

et al. 2023

Genes

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Background: Stem cells have been associated with self-renewing and plasticity and have been investigated in various odontogenic lesions in association with their pathogenesis and biological behavior. We aim to provide a systematic review of stem cell markers’ expression in odontogenic tumors and cysts. Methods: The literature was searched through the MEDLINE/PubMed, EMBASE via OVID, Web of Science, and CINHAL via EBSCO databases for original studies evaluating stem cell markers’ expression in different odontogenic tumors/cysts, or an odontogenic disease group and a control group. The studies’ risk of bias (RoB) was assessed via a Joanna Briggs Institute Critical Appraisal Tool. Meta-analysis was conducted for markers evaluated in the same pair of odontogenic tumors/cysts in at least two studies. Results: 29 studies reported the expression of stem cell markers, e.g., SOX2, OCT4, NANOG, CD44, ALDH1, BMI1, and CD105, in various odontogenic lesions, through immunohistochemistry/immunofluorescence, polymerase chain reaction, flow cytometry, microarrays, and RNA-sequencing. Low, moderate, and high RoBs were observed in seven, nine, and thirteen studies, respectively. Meta-analysis revealed a remarkable discriminative ability of SOX2 for ameloblastic carcinomas or odontogenic keratocysts over ameloblastomas. Conclusion: Stem cells might be linked to the pathogenesis and clinical behavior of odontogenic pathologies and represent a potential target for future individualized therapies.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

The Stem Cell Expression Profile of Odontogenic Tumors and Cysts: A Systematic Review and Meta-Analysis

Kalogirou,

Lekakis,

Petroulias

et al. 2023

Genes

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“…Recently, another diagnosis approach was proposed in relation to immunohistochemistry and biomarkers—Maruyama et al observed that CK13 was exclusively expressed in GOC lesions in comparison to central mucoepidermoid carcinoma, while CK17 expression was related to malignancies [ 12 ]. Moreover, Junior et al [ 25 ] compared the expression of three different biomarkers of GOC, odontogenic keratocyst and botryoid odontogenic cyst and found that high expression of epidermal growth factor receptor (EGFR) was related to botryoid odontogenic cysts and GOCs presented higher expression of nuclear Cyclin D1. Furthermore, Kaplan et al suggest that Ki-67 and p53 biomarkers can help in differentiating GOC from low-grade mucoepidermoid carcinoma [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

Glandular Odontogenic Cyst in the Anterior Mandible: A Case Report of a Conservative Approach and a Recurrence Detection

et al. 2023

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Purpose: The glandular odontogenic cyst (GOC) is considered a rare developmental cyst, with an odontogenic origin and both epithelial and glandular characteristics, with less than 200 reported cases in the literature. Methods: In the present case, a 29-year-old man was referred for evaluation of an asymptomatic slow-growing swelling in the anterior region of the mandible, with one-year history. The patient’s medical history did not reveal any systemic alteration. The extraoral examination did not show enlargement of the facial contour and the intraoral examination showed vestibular and lingual swelling. Panoramic radiography and CT scan revealed a well-defined unilocular radiolucent lesion involving the inferior incisors and canines bilaterally. Results: Histopathological analysis revealed multiple cysts lined by stratified epithelium with varying thickness and characteristics, in addition to duct-like structures filled with PAS-positive amorphous material, suggestive of GOC. Conservative treatment was performed through surgical curettage, peripheral ostectomy of the surgical site and apicectomy of the teeth involved in the lesion. There was one recurrence, which was detected in postoperative follow-up, leading to a new surgical approach. Conclusions: Fifteen months after the second procedure, no signs of recurrence were identified, and bone neoformation within the surgical site occurred, supporting that a conservative approach for the treatment of GOC is viable.

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“…Though the results are partially promising, the exact molecular mechanism of invasion in ameloblastomas is not completely understood. 6 Cell motility is essential for tumor invasion and subsequent dissemination or metastases. This increase in motility occurs via the modulation of actin filaments to form finger-like plasma membrane protrusions termed invadopodia.…”

Section: Discussionmentioning

confidence: 99%

“…Literature review reveals many preliminary observations with no concrete evidence of a single marker being specific to these tumors and hence there is a need to determine new markers. 6 In this study, we have employed two markers: fascin and SALL4. Fascin, a 55-kDa is a cytoskeleton binding proteins that bundle actin filaments, assists the cell in forming stress fibres (or ruffled borders or micro spikes) and assists cell motility and migration hence it fascin can be used for predicting the aggressive clinical course of a tumor.…”

Section: Introductionmentioning

confidence: 99%

Expression of Fascin and SALL4 in odontogenic cysts and tumors: an immunohistochemical appraisal.

et al. 2022

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Background: Various stemness markers (SOX2, OCT4, and NANOG) have been studied in odontogenic cysts and tumors. However, studies on SALL4 having similar properties of stemness has not been documented. Additionally, insight into fascin as a migratory molecule is less explored. In this study, the expression of SALL4 and fascin were evaluated in ameloblastoma, adenomatoid odontogenic tumor (AOT), odontogenic keratocyst (OKC), dentigerous cyst (DC), radicular cyst (RC), and calcifying odontogenic cyst (COC). Methods: Semi-quantitative analysis of fascin and SALL4 immuno-positive cells was done in a total of 40 cases of ameloblastoma (11 plexiform, 12 follicular, 12 unicystic, and 5 desmoplastic) variants, 6 cases of AOT, 15 each of OKC, DC, RC and 5 of COC. Chi-square test was applied to evaluate the association between SALL4 and fascin expression in odontogenic cysts and tumors. Results: Fascin immunopositivity was observed in peripheral ameloblast-like cells, and weak or absent in stellate reticulum-like cells. A moderate to weak immune-reactivity to SALL4 was observed in the cytoplasm of ameloblastoma, epithelial cells of dentigerous and radicular cysts, having a marked inflammatory infiltrate, which is an interesting observation. COC and AOT had negative to weak expressions. No recurrence has been reported. Conclusions: Expression of fascin in ameloblastomas elucidate their role in motility and localized invasion. Its expression in less aggressive lesions like DC, COC, AOT will incite to explore the other functional properties of fascin. SALL4 expression in the cytoplasm of odontogenic cysts and tumors may represent inactive or mutant forms which requires further validation.

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Biomarkers involved in the proliferation of the odontogenic keratocyst, glandular odontogenic cyst and botryoid odontogenic cyst

Cited by 7 publications

References 38 publications

The Stem Cell Expression Profile of Odontogenic Tumors and Cysts: A Systematic Review and Meta-Analysis

The Stem Cell Expression Profile of Odontogenic Tumors and Cysts: A Systematic Review and Meta-Analysis

Glandular Odontogenic Cyst in the Anterior Mandible: A Case Report of a Conservative Approach and a Recurrence Detection

Expression of Fascin and SALL4 in odontogenic cysts and tumors: an immunohistochemical appraisal.

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