Biomarkers in uterine leiomyoma

Levy, Gary; Hill, Micah J.; Plowden, Torie C.; Catherino, William H.; Armstrong, Alicia

doi:10.1016/j.fertnstert.2012.10.048

Cited by 34 publications

(33 citation statements)

References 59 publications

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“…Based on the multiplied value of the AUC and a cross‐validation score, the best combination was determined, which consisted of two miRNAs (miR‐191‐5p and miR‐1246), and the diagnostic index (DI) of the model was defined as (DI = (−0.479) × miR‐191‐5p + (−0.380) × miR‐1246 + 6.386). When distinguishing all US subtypes from benign ULM controls, the diagnostic performance of the model was higher than that of serum lactate dehydrogenase (LDH), which is a major conventional biomarker for US (model‐AUC: 0.83 [0.65‐1.00], and LDH‐AUC: 0.62 [0.38‐0.85]) (Figure ). Notably, when focusing on ULMS screening, the accuracy was much higher and the difference from the LDH level was more significant (model‐AUC: 0.97 [0.91‐1.00], and LDH‐AUC: 0.64 [0.34‐0.94]) (Figure A).…”

Section: Resultsmentioning

confidence: 99%

Serum microRNA profile enables preoperative diagnosis of uterine leiomyosarcoma

Yokoi

Matsuzaki²,

Yamamoto³

et al. 2019

Cancer Science

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Uterine leiomyosarcoma (ULMS) is the major subtype of uterine sarcoma (US) and contributes to uterine cancer deaths. Although preoperative diagnosis of US remains challenging, frequent application of laparoscopic surgery for benign uterine leiomyomas (ULM) requires precise exclusion of US. MicroRNAs are stably present in the bloodstream, and the application of circulating miRNAs as disease biomarkers has been recognized. In the present study, we aimed to identify diagnostic biomarkers for distinguishing US from ULM by focusing on circulating miRNAs. All serum samples were collected preoperatively between 2009 and 2017, and all cases were histopathologically diagnosed. Whole miRNA profiles were obtained using a miRNA microarray. By analyzing expression levels of the miRNAs, candidate miRNAs were selected based on diagnostic performance in discriminating US from ULM, and a diagnostic model was then constructed. A total of 90 serum samples were analyzed, and clustering analyses revealed that the profiles of ULMS were distinct from those of controls. Based on leave-one-out cross-validation, seven miRNAs were selected as biomarker candidates. Based on model construction, the optimal model consisted of two miRNAs (miR-1246 and miR-191-5p), with an area under the receiver operating characteristic curve (AUC) for identifying ULMS of 0.97 (95% confidence interval [CI], 0.91-1.00). In contrast, serum lactate dehydrogenase had an AUC of only 0.64 (95% CI, 0.34-0.94). Seven serum miRNAs with high diagnostic performance for preoperative US screening were detected, and a promising diagnostic model for ULMS was generated. K E Y W O R D Scirculating microRNA, diagnostic biomarkers, liquid biopsy, uterine leiomyosarcoma, uterine sarcoma | 3719 YOKOI et al.

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Section: Resultsmentioning

confidence: 99%

Serum microRNA profile enables preoperative diagnosis of uterine leiomyosarcoma

Yokoi

Matsuzaki²,

Yamamoto³

et al. 2019

Cancer Science

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“…Similarly, Sakaguchi et al [18] showed that coordinated expression of ERα and ERβ might be necessary for normal estrogen action in myometrium [19]. In addition, it has been shown ERα is phosphorylated at a higher rate on serine in leiomyoma compared with surrounding myometrium, for that reason it is possible that phosphorylated ERα regulated by p44/42 MAPK, will have a role in development of uterine leiomyoma [20,21].…”

Section: Discussionmentioning

confidence: 95%

The influence of the expression of steroid receptors on angiogenesis, proliferation and apoptosis in myomas of pre- and postmenopausal women

et al. 2019

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Introduction/Objective The aim of this study was to determine the effects of the estrogen and progesterone receptor status on angiogenesis, proliferation, and apoptosis of myoma cells in premenopausal (PreM) and postmenopausal (PostM) women. Methods This was a cross section; clinical-experimental, retrospective, non-interventional study in the field of the study of fundamental pathogenesis mechanisms of disease using pathohistological materials from the existing archive. The research included 76 patients diagnosed with uterine leiomyomas, operatively treated in the Clinic for Gynecology and Obstetrics, Clinical Centre Kragujevac, Serbia. According to the menstrual status, we formed two experimental subgroups. The first group was PreM women (n = 35; 46.2 ± 5.02 years old), and the second group was PostM women (n = 41; 60.25 ± 5.41 years old). Hematoxylin-eosin staining for myoma and myometrium was conducted, as well as immunohistochemistry for ERα, ERβ, PRа, vascular endothelial growth factor, endoglin, Ki67, and caspase-3. Results Progesterone receptor was overexpressed in myoma and myometrium of PreM compared to myoma and myometrium of PostM women. Expression of caspase-3 was a statistically significant increase in PostM women compared to PreM group. ERα and ERβ were not changed among groups neither in myoma nor in myometrium samples. Conclusion According to our data, PRа had higher influence on apoptosis and cell growth than estrogen receptors. Since PRа was increased in PreM in both myoma and myometrium, probably this expression led further to lower expression of apoptotic marker in PreM women.

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“…Our patient had no clinical or radiological ascites, and given her previous history of laparoscopic myomectomy, we did not consider measuring the tumor markers, because the mass was unlikely to be malignant. However, in cases of suspected malignancy such as leiomyosarcoma, tumor markers may be useful in monitoring therapy and follow-up, especially if initially elevated [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

Port site parasitic leiomyoma after laparoscopic myomectomy: a case report and review of the literature

2018

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BackgroundUterine fibroids are the commonest benign gynecological tumors. Laparoscopic myomectomy is becoming increasingly popular as one of the surgical treatment options for symptomatic cases. Large tissues such as leiomyomas or even the uterus need to be morcellated in order to be retrieved from the abdominal cavity. Some of the morcellated fragments or small fibroids may be accidentally left in the abdominal cavity during the retrieval process. These may subsequently become implanted in the abdominal cavity, develop blood supply from the surrounding structures, and grow to form parasitic myomas with varied clinical presentation, depending on the location and size.Case presentationA 47-year-old African woman presented to our hospital 6 years after laparoscopic myomectomy with a lower abdominal mass. Her work-up revealed an anterior abdominal wall mass consistent with uterine leiomyoma. She was scheduled for excision of the mass, which was subsequently histologically confirmed to be a uterine fibroid.ConclusionsParasitic leiomyomas are a rare late complication of power morcellation following laparoscopic myomectomy or hysterectomy. Most patients present with an abdominal/pelvic mass and may need surgical excision to relieve the symptoms. Care should be taken during power morcellation to prevent excessive fragmentation of the tissues, some of which may become implanted and persist to form parasitic myomas. Moreover, effort should be made to retrieve all myoma fragments by carefully checking the abdominal cavity. Whenever possible, the morcellation should be done in a containment bag.

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Biomarkers in uterine leiomyoma

Cited by 34 publications

References 59 publications

Serum microRNA profile enables preoperative diagnosis of uterine leiomyosarcoma

Serum microRNA profile enables preoperative diagnosis of uterine leiomyosarcoma

The influence of the expression of steroid receptors on angiogenesis, proliferation and apoptosis in myomas of pre- and postmenopausal women

Port site parasitic leiomyoma after laparoscopic myomectomy: a case report and review of the literature

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