Biomarkers in marine mussels, Mytilus galloprovincialis, exposed to environmentally relevant levels of the pesticides, chlorpyrifos and penoxsulam

Patetsini, E.; Dimitriadis, V.K.; Kaloyianni, Martha

doi:10.1016/j.aquatox.2012.09.009

Cited by 98 publications

(29 citation statements)

References 63 publications

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“…In addition, covalent binding to certain breakdown products of lipid hydroperoxides, such as malondialdehyde (MDA), can result in DNA strand breaks and crosslinks (Cheung et al, 2002), while it is wellknown that carbonyl compounds are toxic due to their carcinogenic properties (Labieniec and Gabryelak, 2004). The significant positive correlation (r = 0.88; p b 0.05) between primary DNA damage and protein carbonylation found in our study suggested the involvement of carbonylated proteins in Δ-9-THC genotoxicity and agreed data by Patetsini et al (2013), who found an increase of DNA fragmentation linked to protein carbonyl content in M. galloprovincialis specimens treated with two pesticides. Although several studies showed that DNA strand breaks are apoptosis-inducing factor in zebra mussels (Binelli et al, 2009a,b;Parolini and Binelli, 2012) and the main contributor to micronuclei induction (Van Goethem et al, 1997), only a significant increase (p b 0.01) of micronucleated cell frequency (Fig.…”

Section: Discussionsupporting

confidence: 91%

“…2a) showed a progressive aggravation of the bivalve health status, confirming that treated specimens suffered a cellular stress situation likely linked to the induction of oxidative stress (Lowe et al, 1995), mainly at the highest concentration. This condition could lead to notable oxidative damage to different cellular macromolecules, such as lipids of membranes, proteins and DNA, as observed in Mediterranean mussels exposed to environmentally relevant levels of the pesticides chlorpyrifos and penoxsulam (Patetsini et al, 2013). The measure of lipid peroxidation, a traditional end-point of oxidative stress (Tedesco et al, 2010), and protein carbonyl content of tissues, the most general indicator of severe protein oxidative damage (Dalle-Donne et al, 2003), supported this hypothesis.…”

Section: Discussionmentioning

confidence: 61%

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Oxidative and genetic responses induced by Δ-9-tetrahydrocannabinol (Δ-9-THC) to Dreissena polymorpha

Parolini

Binelli

2014

Science of The Total Environment

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 61%

Oxidative and genetic responses induced by Δ-9-tetrahydrocannabinol (Δ-9-THC) to Dreissena polymorpha

Parolini

Binelli

2014

Science of The Total Environment

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“…Thus, moderately damaged comets are those in classes 1 (rate of damage 5-20 %) and 2 (rate of damage 20-40 %), while highly damaged comets are those in classes 3 (rate of damage 40-75 %) and 4 (rate of damage [75 %). This criterion has been used in other publications by our group (Miranda-Vilela et al 2010;Rivero-Wendt et al 2013), as well as by other groups (Cuong et al 2006;Patetsini et al 2013).…”

Section: Resultsmentioning

confidence: 99%

Genotoxic evaluation in Oreochromis niloticus (Fish: Characidae) of recombinant spore–crystal complexes Cry1Ia, Cry10Aa and Cry1Ba6 from Bacillus thuringiensis

et al. 2013

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Bioinsecticides from Bacillus thuringiensis (Bt) are widely used around the world in biological control against larval stages of many insect species. Bt has been considered a biopesticide that is highly specific to different orders of insects, non-polluting and harmless to humans and other vertebrates, thus becoming a viable alternative for combating agricultural pests and insect vectors of diseases. The family of Bt δ-endotoxins are crystal-protein inclusions showing toxicity to insects' midgut, causing cell lysis leading to starvation, septicemia and death. The aim of this study is to evaluate the genotoxic potential of recombinant Bt spore-crystals expressing Cry1Ia, Cry10Aa and Cry1Ba6 on peripheral erythrocyte cells of Oreochromis niloticus, through comet assay, micronucleus (MN) test and nuclear abnormalities (NA) analysis. Fish (n = 10/group) were exposed for 96 h at 10(7) spores 30 l(-1), 10(8) spores 30 l(-1) or 10(9) spores 30 l(-1) of Bt spore-crystals. Cry1Ia showed a significant increase in comet cells at levels 1 and 2, but not at levels 3 and 4, so it was not mutagenic nor did it induce MN or NA. These three spore-crystals showed some fish toxicity at only the highest exposure level, which normally does not occur in the field.

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“…Although pesticides may demonstrated some effects on hemocytes after long exposure in in vivo experiments (Patetsini et al, 2013), testing of long exposure period is difficult in in vitro assays as hemocytes do not survive for long time outside of oysters.…”

Section: Discussionmentioning

confidence: 99%

Pacific oyster (Crassostrea gigas) hemocyte are not affected by a mixture of pesticides in short-term in vitro assays

Moreau

Burgeot

Renault

2013

Environ Sci Pollut Res

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Pesticides are frequently detected in estuaries among the pollutants found in estuarine and coastal areas and may have major ecological consequences. They could endanger organism growth, reproduction, or survival. In the context of high-mortality outbreaks affecting Pacific oysters, Crassostrea gigas, in France since 2008, it appears of importance to determine the putative effects of pesticides on oyster susceptibility to infectious agents. Massive mortality outbreaks reported in this species, mainly in spring and summer, may suggest an important role played by the seasonal use of pesticides and freshwater input in estuarine areas where oyster farms are frequently located. To understand the impact of some pesticides detected in French waters, their effects on Pacific oyster hemocytes were studied through short-term in vitro experiments. Bivalve immunity is mainly supported by hemocytes eliminating pathogens by phagocytosis and producing compounds including lysosomal enzymes and antimicrobial molecules. In this study, oyster hemocytes were incubated with a mixture of 14 pesticides and metaldehyde alone, a molecule used to eliminate land mollusks. Hemocyte parameters including dead/alive cells, nonspecific esterase activities, intracytoplasmic calcium, lysosome number and activity, and phagocytosis were monitored by flow cytometry. No significant effect of pesticides tested at different concentrations was reported on oyster hemocytes maintained in vitro for short-term periods in the present study. It could be assumed that these oyster cells were resistant to pesticide exposure in tested conditions and developing in vivo assays appears as necessary to better understand the effects of pollutants on immune system in mollusks.

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Biomarkers in marine mussels, Mytilus galloprovincialis, exposed to environmentally relevant levels of the pesticides, chlorpyrifos and penoxsulam

Cited by 98 publications

References 63 publications

Oxidative and genetic responses induced by Δ-9-tetrahydrocannabinol (Δ-9-THC) to Dreissena polymorpha

Oxidative and genetic responses induced by Δ-9-tetrahydrocannabinol (Δ-9-THC) to Dreissena polymorpha

Genotoxic evaluation in Oreochromis niloticus (Fish: Characidae) of recombinant spore–crystal complexes Cry1Ia, Cry10Aa and Cry1Ba6 from Bacillus thuringiensis

Pacific oyster (Crassostrea gigas) hemocyte are not affected by a mixture of pesticides in short-term in vitro assays

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