Biomarkers for atopic dermatitis

Thijs, Judith L.; Krastev, Todor K.; Weidinger, Stephan; Buckens, Constantinus F.; Bruin‐Weller, Marjolein de; Bruijnzeel-Koomen, Carla A.F.M.; Flohr, Carsten; Hijnen, DirkJan

doi:10.1097/aci.0000000000000198

Cited by 194 publications

(89 citation statements)

References 95 publications

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“…SC tapes and interstitial fluid) are consistent with those from blood samples. Utilizing a multiplex analysis, Thijs et al [43] found a significant decrease in the levels of 7 out of 31 investigated biomarkers, including CCL17 and CCL22, in blood samples from patients with moderate-to-severe AD who were treated with a potent corticosteroid [44]. This is in line with the results of the present study in which a significant decrease in CCL17 and IL-8 was found after the therapy with the emollient containing magnesium and ceramides.…”

Section: Discussionsupporting

confidence: 82%

“…This is in line with the results of the present study in which a significant decrease in CCL17 and IL-8 was found after the therapy with the emollient containing magnesium and ceramides. CCL17 and CCL22 act via the Th2 cell chemokine receptor CCR4 [44,45,46,47] . In a recent meta-analysis, serum levels of CCL17 were the most reliable available biomarker of AD disease severity, showing correlation coefficients of 0.60 (95% CI 0.48-0.70) and 0.64 (95% CI 0.57-0.70) in longitudinal and cross-sectional studies, respectively [41,43,44,48,49].…”

Section: Discussionmentioning

confidence: 99%

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Stratum Corneum Tape Stripping: Monitoring of Inflammatory Mediators in Atopic Dermatitis Patients Using Topical Therapy

Koppes

Brans

Hadžavdić

et al. 2016

Int Arch Allergy Immunol

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Objective: The aim of this study was to explore the tape strip sampling technique in the assessment of stratum corneum levels of inflammatory mediators in a clinical trial setting. Methods: Thirty-eight inflammatory mediators were analyzed by a multiplex-assay in the stratum corneum, collected by adhesive tapes before and after 6 weeks of therapy, in mild and moderate atopic dermatitis (AD) patients (n = 90). Treatment was a ceramide- and magnesium-containing emollient. Results: Twenty-four mediators could quantitatively be determined. The Th2 mediators interleukin (IL)-4, IL-13, CCL2 (monocyte chemotactic protein-1), CCL22 (macrophage-derived chemokine), and CCL17 [thymus and activation-regulated chemokine (TARC)] were significantly decreased after therapy as well as IL-1β, IL-2, IL-8 (CXCL8), IL-10, acute-phase protein serum amyloid A, C-reactive protein, and vascular adhesion molecule-1. The decrease of CCL17 and IL-8 was correlated with the decrease in disease severity in a subgroup of moderate AD individuals. Conclusion: Stratum corneum tape stripping offers a minimally invasive approach for studying local levels of immunomodulatory molecules in the skin. CCL17 (TARC) and IL-8 were found to be the most promising biomarkers of AD and might be useful for investigating the course of skin diseases and the effect of local therapy.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Stratum Corneum Tape Stripping: Monitoring of Inflammatory Mediators in Atopic Dermatitis Patients Using Topical Therapy

Koppes

Brans

Hadžavdić

et al. 2016

Int Arch Allergy Immunol

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“…Recently, IL-18 levels in the maternal serum and cord blood were found to be a predisposing factor of childhood AD [152]. Elevated levels of serum IL-18 and IL-18 receptors have been suggested as biomarkers of AD severity [146,153], but additional research is required to confirm this suggestion [154,155]. …”

Section: Immunological Abnormalitiesmentioning

confidence: 99%

“…Furthermore, increased CCL17 levels in cord blood are associated with the development of infantile AD [182]. A recent meta-analysis indicated that serum CCL17 is the most reliable biomarker identified to date [154]. Similar to CCL17, macrophage-derived chemokine, also known as CCL22, is a chemoattractant for CCR4-expressing T cells.…”

Section: Immunological Abnormalitiesmentioning

confidence: 99%

Molecular Mechanisms of Cutaneous Inflammatory Disorder: Atopic Dermatitis

Kim

Cho

et al. 2016

IJMS

106

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Atopic dermatitis (AD) is a multifactorial inflammatory skin disease resulting from interactions between genetic susceptibility and environmental factors. The pathogenesis of AD is poorly understood, and the treatment of recalcitrant AD is still challenging. There is accumulating evidence for new gene polymorphisms related to the epidermal barrier function and innate and adaptive immunity in patients with AD. Newly-found T cells and dendritic cell subsets, cytokines, chemokines and signaling pathways have extended our understanding of the molecular pathomechanism underlying AD. Genetic changes caused by environmental factors have been shown to contribute to the pathogenesis of AD. We herein present a review of the genetics, epigenetics, barrier dysfunction and immunological abnormalities in AD with a focus on updated molecular biology.

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“…Although the majority of AD patients have highly increased total IgE levels, these levels do not correlate with disease severity [3]. During the synthesis of immunoglobulins, light chains are produced in excess over heavy chains [4, 5].…”

Section: Introductionmentioning

confidence: 99%

Immunoglobulin free light chains in adult atopic dermatitis patients do not correlate with disease severity

Thijs

Knipping

Bruijnzeel-Koomen

et al. 2016

Clin Transl Allergy

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BackgroundAlthough total IgE levels have been proposed as a biomarker for disease severity in atopic dermatitis (AD) and are increased in the majority of AD patients, they do not correlate with disease severity during short-term follow-up. During the synthesis of immunoglobulins, free light chains (Ig-FLCs) are produced in excess over heavy chains. In comparison with IgE molecules, Ig-FLCs have a very short serum half-life. Therefore, Ig-FLCs might be more suitable as a biomarker for disease severity during follow-up. Recent studies showed increased serum levels of kappa Ig-FLCs in infants with AD, correlating with disease severity. The aim of this study was to investigate serum kappa Ig-FLC levels in adults with AD, and their correlation to disease severity.MethodsSerum kappa If-FLC and total IgE levels were measured in 82 moderate to severe AD patients and 49 non-atopic controls. Blood was collected from patients before start of treatment with potent topical steroids (European classification: III–IV). 32 patients were treated during a clinical admission, and in this subpopulation a second blood sample was taken after 2 weeks of treatment. Clinical severity was determined by the Six Area Six Sign Atopic Dermatitis (SASSAD) severity score and a panel of serum biomarkers, including thymus and activation-regulated chemokine (TARC).ResultsSerum kappa Ig-FLCs levels in adult AD patients were not increased compared to non-atopic controls. Moreover, we observed no correlation between kappa Ig-FLC serum levels and disease severity determined by SASSAD and a panel of serum biomarkers, including TARC. Serum kappa Ig-FLC levels did also not decrease during treatment.ConclusionThere are no differences in serum kappa Ig-FLC levels between adult patients suffering from moderate to severe AD compared to non-atopic controls. Moreover, serum levels of kappa Ig-FLCs cannot be used as a biomarker for disease severity in adult AD.

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Biomarkers for atopic dermatitis

Cited by 194 publications

References 95 publications

Stratum Corneum Tape Stripping: Monitoring of Inflammatory Mediators in Atopic Dermatitis Patients Using Topical Therapy

Stratum Corneum Tape Stripping: Monitoring of Inflammatory Mediators in Atopic Dermatitis Patients Using Topical Therapy

Molecular Mechanisms of Cutaneous Inflammatory Disorder: Atopic Dermatitis

Immunoglobulin free light chains in adult atopic dermatitis patients do not correlate with disease severity

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