Biomarkers and bioaccumulation of clam Ruditapes philippinarum in response to combined cadmium and benzo[α]pyrene exposure

Wang, Lin; Pan, Luqing; Liu, Na; Liu, Dong; Xu, Chaoqun; Miao, Jingjing

doi:10.1016/j.fct.2011.06.015

Cited by 79 publications

(30 citation statements)

References 42 publications

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“…To date, most researchers have focused on characterization of sHSP function in single stress responses, but there are a few studies characterizing the toxic effects of complex pollution exposures. Wang et al (2011) indicated that coexposure to Cd and B[a]P greatly enhanced bioaccumulation of these compounds in Ruditapes philippinarum, which suggested that accumulated Cd and B[a]P in clams apparently interact with each other and may, therefore, greatly modify the toxic effect of the individual chemicals.Marine organisms from coastal waters experience environmental challenges that generate stressful conditions (Zhang et al 2012). Bivalve mollusks are ideal for assessments of levels of environmental pollution in coastal and estuarine areas because they are ubiquitous, sedentary, and filter feeders (Boening 1999, Zhang et al 2012.…”

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confidence: 99%

Identification of a small HSP gene from hard clam Meretrix meretrix and its potential as an environmental stress biomarker

Zhang¹,

Liu²,

He³

et al. 2013

Aquat. Biol.

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Heat shock proteins (HSPs) are molecular chaperones that help organisms cope with stressful conditions. In this study, a novel small HSP (sHSP) gene was identified from the hard clam Meretrix meretrix (designated as Mm-HSP20). The full length of the cDNA is 1222 bp, consisting of a 5'-terminal untranslated region (5'UTR) of 103 bp, a 3'UTR of 565 bp, and a 522 bp open reading frame encoding a polypeptide of 173 amino acids. Sequence comparison showed that Mm-HSP20 had a moderate degree of homology to the sHSP of other organisms. A sHSP feature domain, an alpha-crystallin domain, and a V/IXI/V motif in the C-terminal extension were identified in the Mm-HSP20 amino acid sequence, indicating that Mm-HSP20 is a new member of the sHSP family. The Mm-HSP20 transcript was constitutively expressed in 6 different test tissues, with the highest expression level detected in the digestive gland by fluorescent quantitative realtime PCR. The temporal expression of Mm-HSP20 mRNA in the digestive gland and hemocytes was evaluated after exposure to cadmium (Cd) (40 µg l −1 CdCl 2 ) and benzo [a]pyrene (50 µg l −1 B[a]P) individually, and in combination (40 µg l −1 CdCl 2 + 5 0 µg l −1 B[a]P). Mm-HSP20 expression level increased significantly after Cd and B[a]P exposure in both tissue types. Conversely, significant Mm-HSP20 transcript repression was detected in the 24 h B[a]P-treated digestive gland samples. Considerable up-regulation of Mm-HSP20 mRNA level was observed after multiple exposures. These results indicate that Mm-HSP20 plays a role in mechanisms involved in coping with environmental stress in hard clams. Mm-HSP20 may be suitable for use as a biomarker for heavy metal and B[a]P contamination. KEY WORDS: Hard clam · Meretrix meretrix · HSP20 · mRNA expression · Heavy metal · Cd · Benzo[a]pyrene · BiomarkerResale or republication not permitted without written consent of the publisher Aquat Biol 18: 243-252, 2013 sources, they bioaccumulate, and they are not biodegradable (Zhang et al. 2006). Among these, cadmium (Cd) is one of the most abundant and ubiquitously distributed toxic elements found in aquatic systems (Yuan et al. 2004, Wan et al. 2008). The concentrations of heavy metals observed in coastal sediments are several times higher than those in water columns, and they can have considerable adverse effects on marine organisms. The bioaccumulation of sediment-bound metals in benthic species is extremely important in food webs and ultimately to humans (Wang 2002).Benzo [a]pyrene (B[a]P) is a representative polycyclic aromatic hydrocarbon (PAH). It is therefore classified as a pervasive environmental contaminant. B[a]P is found in water columns and sediments (Men et al. 2009). It enters marine environments via anthropogenic sources, including petroleum spillage, industrial discharge, and urban runoff (Soclo et al. 2000, Boonyatumanond et al. 2006. B[a]P bioaccumulation causes damage to various organ systems in exposed aquatic organisms. Toxicological studies have demonstrated the effects of B[a]P on organ...

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mentioning

confidence: 99%

Identification of a small HSP gene from hard clam Meretrix meretrix and its potential as an environmental stress biomarker

Zhang¹,

Liu²,

He³

et al. 2013

Aquat. Biol.

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“…Moschino et al (2012) noted that R. philippinarum is a good sentinel species for the study of environmental pollution, especially in bioaccumulation and biomarker studies. There are several studies on the bioaccumulation in R. philippinarum for metals (Baudrimont et al, 2005;Figueira et al, 2012;Giani et al, 2012), polychlorinated biphenyls (PCBs) and pesticides (Choi et al, 2014), benzo[a]pyrene (Wang et al, 2011;Liu et al 2014) …”

Section: Hebei Spirit Oil Spill Occurred Near the Taeanmentioning

confidence: 99%

“…Since the embryo of bivalves is highly sensitive to environmental contaminants such as metals, pesticides and organic pollutants, standard toxicity test methods for bivalve embryo are well established (ASTM, 1994;USEPA, 1995). Many kinds of biomarkers in bivalves are also analyzed for evaluating the exposure levels and potential biological effects of toxic contaminants (Maioli et al, 2010;Wang et al, 2011;Moschino et al, 2012). In contrast to embryo stage, adult stage of bivalves is not widely used for routine toxicity tests.…”

Section: Introductionmentioning

confidence: 99%

Bioaccumulation of polycyclic aromatic hydrocarbons in Manila clam (Ruditapes philippinarum) exposed to crude oil-contaminated sediments

Lee¹,

Lee²,

Sung³

et al. 2014

The Korean Journal of Malacology

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The bioaccumulation of 16 United States Environmental Protection Agency (USEPA) priority polycyclic aromatic hydrocarbons (PAHs) and alkylated PAHs in the Manila clam, Ruditapes philippinarum exposed to sediments artificially contaminated by Iranian Heavy Crude Oil was measured and the biota-sediment accumulation factor (BSAF) was estimated through laboratory experiments. The proportion of 16 PAHs accumulated in the tissue of R. philippinarum was only from 3 to 7% of total PAHs. Among 16 PAHs, the concentration of naphthalene was highest in the tissue. Alkylated PAHs were highly accumulated more than 93% of total PAHs. The C3 dibenzothiophene was most highly accumulated. The relative composition of alkylated naphthalenes in the tissue of R. philippinarum was lower than in the sediments. In contrast, those of alkylated compounds of fluorenes, phenanthrenes, dibenzothiophenes were higher in the tissue than the sediments. The BSAF for sum of 16 PAHs was 0.11 to 0.13 g carbon/g lipid and that for alkylated PAHs was 0.05 to 0.06 g carbon/g lipid. Naphthalene showed the highest BSAF value. Alkylated PAHs with the same parent compound, BSAF tended to increase with the number of alkylated branch increased, except for alkylated chrysenes. BSAF of total PAHs lies between that of field-based values, and are also similar to those of other persistent organic pollutants (PCBs, DDTs, HCHs). This study provides the BSAF values of individual alkylated PAHs accumulated in R. philippinarum for the first time and will be used as a basis for further understanding the bioaccumulation of organic contaminants in the marine benthic organisms.

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“…Once entering the body system of an organism, PAHs can exert their toxic effect by either the original compound or the resulting production of reactive oxygen species (ROS) during metabolism [1,4], causing lysosomal membrane disruption [5]. It has been demonstrated that the antioxidant system of aerobic organisms is important for eliminating ROS, and the induction of antioxidant enzymes can be considered as a self-defense process of the body to adapt unsafe environment and to reduce toxicity.…”

Section: Introductionmentioning

confidence: 99%

“…These chemical substances accumulate within the bodies of aquatic organisms, causing different toxicological responses [1]. There are 16 polycyclic aromatic hydrocarbons (PAHs) on the the US EPA priority pollutant list [2], including benzo(a)Pyrene (BaP) and chrysene (CHR).…”

Section: Introductionmentioning

confidence: 99%

Immunotoxic effect of Benzo[α]Pyrene and chrysene in juvenile white shrimp Litopenaeus vannamei

2014

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The effects of benzo(a)pyrene (Bap) (0.03, 0.3 and 3 μg L−1) and chrysene (CHR) (0.3, 2.1 and 14.7 μg L−1) on the function of the immune system of juvenile white shrimp Litopenaeus vannamei were determined under laboratory conditions. This included the total hemocyte count (THC) in the hemolymph, phagocytic activityand pro-phenoloxidase (pro-PO) activity of the hemocyte, phenoloxidase (PO) activity, α2-macroglobulin (α2-M) activity, bacteriolytic activity and antibacterial activity in the hemolymph. The results showed that BaP and CHR could inhibit the immune function of L. vannamei significantly under high concentration BaP and CHR exposure. The results of this study indicated that the immunotoxicity of PAHs in a descending order was BaP>CHR. Moreover, the results indicated the THC in hemolymph, pro-PO activity and phagocytic activity of hemocyte, and bacteriolytic activity in hemolymphcould be used as potentially suitable biomarkersfor early warning indication of PAHs toxicity, this could provide useful information for toxic risk assessment of environmental pollutants.

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Biomarkers and bioaccumulation of clam Ruditapes philippinarum in response to combined cadmium and benzo[α]pyrene exposure

Cited by 79 publications

References 42 publications

Identification of a small HSP gene from hard clam Meretrix meretrix and its potential as an environmental stress biomarker

Identification of a small HSP gene from hard clam Meretrix meretrix and its potential as an environmental stress biomarker

Bioaccumulation of polycyclic aromatic hydrocarbons in Manila clam (Ruditapes philippinarum) exposed to crude oil-contaminated sediments

Immunotoxic effect of Benzo[α]Pyrene and chrysene in juvenile white shrimp Litopenaeus vannamei

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