Biomarker studies in northern Bohemia.

Binková, Blanka; Lewtas, Joellen; Míšková, I.; Rössner, Pavel; Černá, Milena; Mračková, Gabriela; Peterková, Kateřina; Mumford, Judy L.; Meyer, Steve; Srám, Radim J.

doi:10.1289/ehp.104-1469612

Cited by 40 publications

(21 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These exposures are similar to those reported for non-smoking subjects who, for occupational reasons, spent much time outdoors in the relatively polluted region of Teplice in the Czech Republic ( Binkova et al, 1996 ). Surprisingly, the corresponding levels for Halkida subjects were similar to, or, for the winter period, higher that those found in Athens ( 56.2 and 32.3 g/m 3 , winter and summer, respectively ).…”

Section: Variables Both Locations Athens Halkidasupporting

confidence: 84%

“…Operation of diverse particle sources can also explain why similar PM 2.5 values, but 3 -fold higher PAH values, were observed in Teplice as compared to Athens ( Binkova et al, 1996 ). For example, it is likely that the intensive use of coal for residential heating in Teplice, as compared to the preponderance of diesel -powered central heating in Athens, may significantly contribute to the high PAH exposures seen at the former location in winter.…”

Section: Variables Both Locations Athens Halkidamentioning

confidence: 90%

See 1 more Smart Citation

Personal exposures to PM2.5 and polycyclic aromatic hydrocarbons and their relationship to environmental tobacco smoke at two locations in Greece

Georgiadis

Stoikidou

Topinka

et al. 2001

J Expo Sci Environ Epidemiol

View full text Add to dashboard Cite

In the context of a large -scale molecular epidemiology study of biomarkers of genotoxicity of air pollution, 24 -h mean personal exposures to airborne PM 2.5 ( particulate matter < 2.5 m ) and associated polycyclic aromatic hydrocarbon ( PAHs ) were measured in 194 non -smoking technical institute students living in the city of Athens, Greece ( an area with moderately high levels of air pollution ) and the nearby small town of Halkida anticipated to have lower pollution levels. Extensive information relevant to the assessment of long -term and recent exposure to PAH was obtained from questionnaires as well as a time ± location ± activity diary ( TLAD ) which was kept by all subjects during a 4 -day observation period. During the last 24 h of this period, subjects underwent personal exposure monitoring for PM 2.5 and PAH, while a sample of blood was donated at the end of this period. All subjects were monitored in this way twice; once during a winter season ( October ± February ) and once during the following summer season ( June ± September ) . Nine subjects with plasma cotinine levels above 20 ng / ml were considered as unreported smokers and excluded from the study. Winter PM 2.5 exposures were lower in Athens ( geometric mean 39.7 g / m 3 ) than Halkida ( geometric mean 56.2 g / m 3 ) (P < 0.001 ) , while there was no significant location difference during the summer ( Athens: geometric mean 32.3 g / m 3 , Halkida: geometric mean 32.9 g / m 3 ; P= 0.79 ) . On the other hand, PAH exposures ( sum of the eight carcinogenic PAHs ) were significantly higher in Athens than in Halkida during the winter ( Athens: geometric mean 8.26 ng / m 3 , Halkida: geometric mean 5.80 ng / m 3 ; P < 0.001 ) as well as during the summer ( Athens: geometric mean 4.44 ng / m 3 , Halkida: geometric mean 1.48 ng / m 3 ; P < 0.001 ) . There was a significant difference in the profile of the PAH exposures at the two locations, the proportion of lighter PAH ( benzo This difference appeared to be related to individual exposure to environmental tobacco smoke ( ETS ) , as indicated by ( a ) the correlation at the individual level between the CHRYS / BPer ratio and declared time of recent exposure to ETS as well as plasma cotinine levels, especially during the winter; ( b ) the parallel variation of the mean levels of all three markers ( declared ETS exposure, cotinine levels, CHRYS / BPer ratio ) among three subgroups of subjects ( Athens subjects who had lowest levels of all three markers; Halkida subjects other than those living in the institute campus area; and Halkida subjects living in the institute campus area who had the highest levels of all three markers ) . This demonstrates that ETS can have a distinctive effect on the PAH exposure profile of subjects exposed to relatively low levels of urban air pollution.

show abstract

Section: Variables Both Locations Athens Halkidasupporting

confidence: 84%

Section: Variables Both Locations Athens Halkidamentioning

confidence: 90%

Personal exposures to PM2.5 and polycyclic aromatic hydrocarbons and their relationship to environmental tobacco smoke at two locations in Greece

Georgiadis

Stoikidou

Topinka

et al. 2001

J Expo Sci Environ Epidemiol

View full text Add to dashboard Cite

show abstract

“…Few prior studies have evaluated associations between ambient air pollution and PAH-DNA binding in newborns. However, a significant association between air pollution and WBC-DNA adduct levels has been reported previously in adult populations (1,21).…”

Section: Discussionmentioning

confidence: 75%

Relationship between ambient air pollution and DNA damage in Polish mothers and newborns.

Whyatt

Santella

Jędrychowski

et al. 1998

Environ Health Perspect

View full text Add to dashboard Cite

Industrialized regions in Poland are characterized by high ambient pollution, including polycyclic aromatic hydrocarbons (PAHs) from coal burning for industry and home heating. In experimental bioassays, certain PAHs are transplacental carcinogens and developmental toxicants. Biologic markers can facilitate evaluation of effects of environmental PAHs on the developing infant. We measured the amount of PAHs bound to DNA (PAH-DNA adducts) in maternal and umbilical white blood cells. The cohort consisted of 70 mothers and newborns from Krakow, Poland, an industrialized city with elevated air pollution. Modulation of adduct levels by genotypes previously linked to risk of lung cancer, specifically glutathione S-transferase Ml (GSTM1) and cytochrome P4501A1 (CYP1A1) Mspl restriction fragment length polymorphism (RFLP), was also investigated. There was a dose-related increase in maternal and newborn adduct levels with ambient pollution at the women's place of residence among subjects who were not employed away from home (p<0.05). Maternal smoking (active and passive) significantly increased maternal (p<0.01) but not newborn adduct levels. Neither CYP1A1 Mspl nor GSTM1 polymorphisms was associated with maternal adducts. However, adducts were significantly higher in newborns heterozygous or homozygous for the CYP1A1 Mspl RFLP compared to newborns without the RFLP (p=0.04).Results indicate that PAH-induced DNA damage in mothers and newborns is increased by ambient air pollution. In the fetus, this damage appears to be enhanced by the CYP1A1 Mspl polymorphism.Environ Health Perspect 106(Suppl 3):821-826 (1998). http.//ehpnetl.niehs.nih.gov/docs/1998/ Suppl-3/821-826whyattlabstract html

show abstract

“…41 ). Binkova et al 42 found in a group of healthy non-smoking women, living in an area polluted by PAHs, the average level of 5.35 and 7.12 %tDNA (DNA-SSBs; medians). Other authors studied a group of policemen who were exposed to combination of PAHs and ultraviolet radiation 43 .…”

Section: Discussionmentioning

confidence: 99%

Genotoxic changes in peripheral lymphocytes after therapeutic exposure to crude coal tar and ultraviolet radiation

Málková¹,

Köhlerová²,

Fiala³

et al. 2016

BIOMED PAP

View full text Add to dashboard Cite

Aims. Goeckerman therapy is based on combined exposure to UV radiation (UVA, UVB) and crude coal tar (PAHs). Some indicators suggest a genotoxic hazard, however, the level of genotoxic risk of the therapy has not yet been investigated sufficiently. This study aims to assesss the genotoxic risk. Methods. The studied group consisted of patients with chronic stable plaque psoriasis treated by Goeckerman therapy (n = 29). Heparin-treated peripheral blood samples were collected one day before the first treatment and immediately after the last procedure. The lymphocytes were isolated from the blood. The level of genotoxicity was evaluated using an alkaline version of the Comet assay which detects DNA single strand breaks (DNA-SSBs), a neutral version of the Comet assay which detects DNA double strand breaks (DNA-DSBs), and using chromosomal aberrations. Results. The level of DNA-SSBs increased insignificantly (median; Q1-Q3): 1.4 (0.4; 0.1-1.4) vs. 2.5 (0.6; 0.3-2.7) %tDNA (P = 0.11) and the level of DNA-DSBs increased significantly: 7.8 (6.5; 3. 4-10.5) vs. 20.7 (19.3; 14.2-24.6) % DNA (P < 0.001). The total number of aberrated cells (P < 0.001) and structurally aberrated cells (P < 0.001) increased significantly. Conclusion. The elevated levels of the DNA-DSBs and the chromosomal aberrations in the peripheral lymphocytes indicated a genotoxic hazard. However, the elevated level of the chromosomal abnormalities was below the upper level of the reference range for healthy Czech adults. While, the genotoxic risk appears to be low, Goeckerman treatment represents a further contribution to the lifetime load of genotoxic factors.

show abstract

Biomarker studies in northern Bohemia.

Cited by 40 publications

References 31 publications

Personal exposures to PM2.5 and polycyclic aromatic hydrocarbons and their relationship to environmental tobacco smoke at two locations in Greece

Personal exposures to PM2.5 and polycyclic aromatic hydrocarbons and their relationship to environmental tobacco smoke at two locations in Greece

Relationship between ambient air pollution and DNA damage in Polish mothers and newborns.

Genotoxic changes in peripheral lymphocytes after therapeutic exposure to crude coal tar and ultraviolet radiation

Contact Info

Product

Resources

About