Biomarker responses in Solea senegalensis exposed to sodium hypochlorite used as antifouling

López-Galindo, Cristina; Vargas‐Chacoff, Luis; Nebot, Enrique; Casanueva, José F.; Rubio, Daniel; Solé, Montserrat; Mancera, Juan Miguel

doi:10.1016/j.chemosphere.2009.11.022

Cited by 44 publications

(24 citation statements)

References 42 publications

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“…However, in the case of the species S. senegalensis, it expresses a much lower AChE activity in muscle than the observed in other teleosts (Varó et al, 2008;Solé et al, 2008a;2010), including other flatfish species (Sturm et al, 1999, Minier et al, 2000 although it coincides with values reported for this same species from Southern Spain (Oliva, 2011). Alternatively to muscle, gills have also probed to be an appropriate tissue for neurotoxicity determination in S. senegalensis juveniles (López-Galindo et al, 2010a, 2010b. However, the activity detected in the present study was very low (Vmax 3.31 nmol/min/mg prot) in contrast to the reported values by López-Galindo et al, (2010a; in which it was 4-times higher, even though all these studies use the same analytical protocol.…”

Section: Discussioncontrasting

confidence: 66%

“…Alternatively to muscle, gills have also probed to be an appropriate tissue for neurotoxicity determination in S. senegalensis juveniles (López-Galindo et al, 2010a, 2010b. However, the activity detected in the present study was very low (Vmax 3.31 nmol/min/mg prot) in contrast to the reported values by López-Galindo et al, (2010a; in which it was 4-times higher, even though all these studies use the same analytical protocol.…”

Section: Discussioncontrasting

confidence: 66%

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Characterization of type “B” esterases and hepatic CYP450 isoenzimes in Senegalese sole for their further application in monitoring studies

Solé

Vega

Varó

2012

Ecotoxicology and Environmental Safety

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In fish, the role that cholinesterases (ChEs) play in tissues other than those implicated in neural activity, as well as the involvement of carboxylesterases (CbEs) and cytochrome P450 isoenzymes (CYPs) in drug metabolism needs investigation. For that, Senegalese sole (Solea senegalensis) specimens were selected for characterization of several type B esterases and hepatic CYPs in order to further use this fish as sentinel. ChEs (acetylcholinesterase (AChE) and pseudocholinesterases (butyrylcholinesterase-BuChE and propionilcholinesterase-PrChE)) and CbEs were measured in brain, plasma, kidney, liver, gonad, muscle and gills. Moreover, seven fluorimetric substrates were selected to study CYP related activities in fish liver. The results showed that AChE was the dominant ChE form in brain whereas pseudocholinesterases were absent in most tissues, as demonstrated by low enzymatic activities using specific substrates and the lack of inhibition by iso-OMPA. Plasma exhibited trace activities of all the esterases assayed and no BuChE activity. CbEs were dominant in liver, but they were also present in kidney and brain. For CbE determination, α-naphtyl acetate (αNA) was seen as the most adequate substrate as it displayed higher enzymatic activities and showed more in vitro sensitivity to the carbamate eserine and the organophosphate pesticide dichlorvos. Alkoxyresorufin-O-dealkylase (EROD and BFCOD) activities, indicative in mammals of CYP1A and CYP3A subfamilies, respectively, were the highest microsomal CYP-related activities in liver. The results of this preliminary work allow us to select the most adequate esterase substrate, tissue and hepatic CYP substrate for further monitoring studies.

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Section: Discussioncontrasting

confidence: 66%

Section: Discussioncontrasting

confidence: 66%

Characterization of type “B” esterases and hepatic CYP450 isoenzimes in Senegalese sole for their further application in monitoring studies

Solé

Vega

Varó

2012

Ecotoxicology and Environmental Safety

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“…This response may be due to increased production of reactive oxygen species (López-Galindo et al, 2010). The decreased activity of the treated fish observed in this work, highlighted by a low to medium linear velocity and mean dispersion, may be due to a behavior response that appears in some situations to increase the ventilation and to eliminate the toxicants from the gills (Gerhardt, 2007).…”

Section: Characterization Of the Behavioral Categoriesmentioning

confidence: 88%

Video-tracking of zebrafish (Danio rerio) as a biological early warning system using two distinct artificial neural networks: Probabilistic neural network (PNN) and self-organizing map (SOM)

et al. 2015

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“…In fish, sodium hypochlorite is capable of causing gill damage (hypertrophy, lamellar fusion) and a decreased response of detoxification mechanisms (catalase and glutathion S-transferase), maybe due to increased production of reactive oxygen species, (López-Galindo et al, 2010a). Bisphenol A is capable of causing vitellogenin induction, gonad structural changes in male, reduced sperm quality, and delayed ovulation in females, (Lahnsteiner et al, 2005;Mandich et al, 2007).…”

Section: Discussionmentioning

confidence: 98%

“…Sodium hypochlorite is extremely toxic to aquatic organisms and particularly damaging to gills (López-Galindo et al, 2010a;López-Galindo et al, 2010b). It can react with organic compounds originating halogenated and chlorination products that are even more powerful oxidants, and have biocide action (López-Galindo et al, 2010a;Emmanuel et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Resilience assessment of a biological early warning system based on the locomotor behavior of zebrafish (Danio rerio)

Fernandes

Amorim

Vasconcelos

et al. 2016

Environ Sci Pollut Res

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With the development of new tools such as biological early warning systems, it becomes extremely important to test their reliability and detection capability. This work aimed at testing the sturdiness of a video tracking system by determining whether the detection capability does not deteriorate over time, after successive exposures of the zebrafish to three different toxicants, namely sodium hypochlorite, bisphenol A, and ethanol. Zebrafish were exposed to the three tested compounds separately (one fish, one toxicant) once a day, for 1 h and 30 m over 9 days, to 9 % of the 96 h LC50 of the respective toxicant. The behavior analysis was based on nine movement descriptor parameters of the fish, namely: angular velocity; linear velocity; spatial dispersion; linear acceleration; and angular acceleration. A statistical method was developed using self-organizing map (SOM), correspondence analysis, and linear and orthogonal multiple regression models. The results indicated that the system was able to successfully detect the three toxicants. With ethanol, the detection capability was maintained, but in the case of the sodium hypochlorite and bisphenol A, a deterioration of the detection capability occurred over the 9 days. This effect may be due to the induction of detoxification mechanisms and physiological acclimation, or due to the accumulation of adverse effects caused by the repeated exposure to the toxicants. Future works, especially those focusing on the application of similar early warning systems in real-world scenarios, should regularly exchange the sentinel organisms, to avoid degradation of the detection capability, as verified with two of the three tested compounds.

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Biomarker responses in Solea senegalensis exposed to sodium hypochlorite used as antifouling

Cited by 44 publications

References 42 publications

Characterization of type “B” esterases and hepatic CYP450 isoenzimes in Senegalese sole for their further application in monitoring studies

Characterization of type “B” esterases and hepatic CYP450 isoenzimes in Senegalese sole for their further application in monitoring studies

Video-tracking of zebrafish (Danio rerio) as a biological early warning system using two distinct artificial neural networks: Probabilistic neural network (PNN) and self-organizing map (SOM)

Resilience assessment of a biological early warning system based on the locomotor behavior of zebrafish (Danio rerio)

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