Biomarker Discovery with Mass Spectrometry Imaging and Profiling

Dunham, Sage J. B.; Neumann, Elizabeth K.; Lanni, Eric J.; Ong, Ta‐Hsuan; Sweedler, Jonathan V.

doi:10.1002/9781119081661.ch4

Cited by 2 publications

(2 citation statements)

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“…MALDI is a common ionization method within the IMS field as it can visualize numerous molecular species over a broad mass range with great molecular diversity. For tissue analysis, sample preparation typically involves sectioning of tissues and thaw mounting these sections onto a glass slide or other target (e.g., conductive surface for high voltage ion sources) and subsequent application of an organic chemical matrix that aids in analyte desorption and ionization . Spatial resolution is defined by the ablation area of the laser and the distance between pixels (pitch).…”

Section: Overview Of Imaging Mass Spectrometry Technologiesmentioning

confidence: 99%

“…tissues and thaw mounting these sections onto a glass slide or other target (e.g., conductive surface for high voltage ion sources) and subsequent application of an organic chemical matrix that aids in analyte desorption and ionization. 40 Spatial resolution is defined by the ablation area of the laser and the distance between pixels (pitch). A variety of matrices are used for specific molecular classes.…”

Section: Introductionmentioning

confidence: 99%

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Multimodal Imaging Mass Spectrometry: Next Generation Molecular Mapping in Biology and Medicine

Neumann

Djambazova

Caprioli

et al. 2020

J. Am. Soc. Mass Spectrom.

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Imaging mass spectrometry has become a mature molecular mapping technology that is used for molecular discovery in many medical and biological systems. While powerful by itself, imaging mass spectrometry can be complemented by the addition of other orthogonal, chemically informative imaging technologies to maximize the information gained from a single experiment and enable deeper understanding of biological processes. Within this review, we describe MALDI, SIMS, and DESI imaging mass spectrometric technologies and how these have been integrated with other analytical modalities such as microscopy, transcriptomics, spectroscopy, and electrochemistry in a field termed multimodal imaging. We explore the future of this field and discuss forthcoming developments that will bring new insights to help unravel the molecular complexities of biological systems, from single cells to functional tissue structures and organs.

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Section: Overview Of Imaging Mass Spectrometry Technologiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%