Biomarker cruises in sepsis: who is the CAPTAIN? Discussion on “Circulating biomarkers may be unable to detect infection at the early phase of sepsis in ICU patients: the CAPTAIN prospective multicenter cohort study”

Briassoulis, George; Briassoulis, Panagiotis; Miliaraki, Marianna; Ilia, Stavroula; Parlato, Marianna; Philippart, François; Rouquette, Alexandra; Moucadel, Virginie; Cavaillon, Jean‐Marc; Misset, Benoît

doi:10.1007/s00134-018-5451-y

Cited by 10 publications

(7 citation statements)

References 7 publications

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“…The non-infectious SIRS group included trauma patients who met at least two of the four conventional criteria for SIRS 59 Assays. Data have been inconsistent regarding survivin isoforms, their cell type expressions or interphase ratios and, since this is a first trial in sepsis 34,60 , survivin was measured in white blood cells, in order to present its total intracellular potential, and in serum as well, so as to assess the comparative extracellular concentrations of the actively secreted survivin, the way other biomolecules and caspases have been previously studied [61][62][63][64][65][66] . Blood samples were obtained within 24 h of admission to the ICU and then stored at −80 °C until total RNA extraction.…”

Section: Discussionmentioning

confidence: 99%

Survivin and caspases serum protein levels and survivin variants mRNA expression in sepsis

Miliaraki

Briassoulis

Ilia

et al. 2021

Sci Rep

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Sepsis is a dysregulated host response to infection related to devastating outcomes. Recently, interest has been shifted towards apoptotic and antiapoptotic pathobiology. Apoptosis is executed through the activation of caspases regulated by a number of antiapoptotic proteins, such as survivin. The survivin and caspases’ responses to sepsis have not yet been elucidated. This is a multicenter prospective observational study concerning patients with sepsis (n = 107) compared to patients with traumatic systemic inflammatory response syndrome (SIRS) (n = 75) and to healthy controls (n = 89). The expression of survivin was quantified through real-time quantitative polymerase chain reaction for the different survivin splice variants (wild type-WT, ΔEx3, 2B, 3B) in peripheral blood leukocytes. The apoptotic or antiapoptotic tendency was specified by measuring survivin-WT, caspase-3, and -9 serum protein concentrations through enzyme-linked immunosorbent assay. The survivin-WT, -2B, -ΔΕx3 mRNA, survivin protein, and caspases showed an escalated increase in SIRS and sepsis, whereas survivin-3B was repressed in sepsis (p < 0.05). Survivin correlated with IL-8 and caspase-9 (p < 0.01). For discriminating sepsis, caspase-9 achieved the best receiver operating characteristic curve (AUROC) of 0.95. In predicting mortality, caspase-9 and survivin protein achieved an AUROC of 0.70. In conclusion, specific apoptotic and antiapoptotic pathways might represent attractive targets for future research in sepsis.

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Section: Discussionmentioning

confidence: 99%

Survivin and caspases serum protein levels and survivin variants mRNA expression in sepsis

Miliaraki

Briassoulis

Ilia

et al. 2021

Sci Rep

Self Cite

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“…Previous publications accentuate that novel biomarkers deliver a solid discriminative competence of among critically ill patients with sepsis which also relates to prognosis. 27,28 Lan et al discover that the diagnostic significance of miR-155-5p and miR-133a-3p with promising sensitivity and specificity, suggesting the potential of specific miRNA as an alternative biomarker in sepsis. 29 CLP-elicited animal models are the most commonly used models in sepsis to explore the mechanism of sepsis.…”

Section: Discussionmentioning

confidence: 99%

MiR-940 Serves as a Diagnostic Biomarker in Patients with Sepsis and Regulates Sepsis-Induced Inflammation and Myocardial Dysfunction

Zhang¹,

Wei²,

Liu³

et al. 2021

JIR

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Introduction: Sepsis is a heterogeneous syndrome with a life-long threat caused by infection. This study aimed to investigate the clinical function of miR-940 and its influence on cardiomyocyte models. Methods: The relative expression of miR-940 was assessed by qRT-PCR and the roles in the clinical diagnosis of miR-940 were revealed by the ROC curve. The relationship between miR-940 and clinical parameters was validated by Pearson analysis. The sepsis rat models were established by treatment with cecal ligation and perforation (CLP) and clinical items including left ventricular systolic pressure (LVSP), left ventricular and end-diastolic pressure (LVEDP), maximum rate of increase/decrease in left ventricular blood pressure (± dp/dt max ) as well as troponin (cTnl), creatine kinase isoenzyme (CK-MB), TNF-α, IL-1β, and IL-6 were detected. Results: The finding of qRT-PCR accentuated that the relative expression of miR-940 was significantly decreased in sepsis patients and CLP-stimulated models. The ROC curve proposed that miR-940 could be a satisfactory diagnostic biomarker for sepsis patients. Pearson analysis reinforced the expression of miR-940 was negatively associated with the PCT, WBC, CRP, Scr, SOFA score, and APACHE II score. The outcome of CLP-steered rat verified that overexpression of miR-940 inhibited the detrimental effects of CLP on myocardial dysfunction and inflammation reactions. Conclusion:The downregulation of miR-940 was reported and it might be an underlying diagnostic marker in sepsis patients. Overexpression of miR-940 protected myocardial function from damage and inflammation induced by CLP.

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“…Procalcitonin (PCT) is the most studied biomarker in sepsis, with a cut-off value of 1.1 ng/ml [sensitivity of 77% and specificity of 79%; area under the receiver operating characteristic curve of 0.85 (95% CI 0·81–0·88)] used for diagnosis of sepsis, depending on pre-test probability [ 11 ]. A single measurement of PCT for early diagnosis is clinically useful when sepsis-3 criteria are used [ 12 , 13 ]. The combination of using sepsis biomarkers and clinical variables, known as ‘bioscores’, improves early detection [ 14 ].…”

Section: Sepsismentioning

confidence: 99%

Biomarkers in the ICU: less is more? No

2020

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Biomarker cruises in sepsis: who is the CAPTAIN? Discussion on “Circulating biomarkers may be unable to detect infection at the early phase of sepsis in ICU patients: the CAPTAIN prospective multicenter cohort study”

Cited by 10 publications

References 7 publications

Survivin and caspases serum protein levels and survivin variants mRNA expression in sepsis

Survivin and caspases serum protein levels and survivin variants mRNA expression in sepsis

MiR-940 Serves as a Diagnostic Biomarker in Patients with Sepsis and Regulates Sepsis-Induced Inflammation and Myocardial Dysfunction

Biomarkers in the ICU: less is more? No

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