Biomarker analysis of American toad (Anaxyrus americanus) and grey tree frog (Hyla versicolor) tadpoles following exposure to atrazine

Snyder, Marcia N.; Henderson, W. Matthew; Glinski, Donna A.; Purucker, S. Thomas

doi:10.1016/j.aquatox.2016.11.018

Cited by 19 publications

(9 citation statements)

References 51 publications

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“…Understanding the mechanisms behind this process and how it governs exposure of pesticides in amphibians is critical in estimating how hydration status interacts with other natural and anthropogenic stressors in specific landscapes (Purucker et al 2007). Interactions of hydration status and pesticide exposure in amphibians also needs to be studied at levels beyond individual body burdens (biomarkers to population) for a broader understanding of the stressors being experienced by amphibians (Grant et al 2016;Snyder et al 2017). Rehydration rates (g/h) of two amphibian species exposed to pesticides after varying periods of dehydration…”

Section: Discussionmentioning

confidence: 99%

Effect of hydration status on pesticide uptake in anurans following exposure to contaminated soils

Glinski

Henderson

Meter

et al. 2018

Environ Sci Pollut Res

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In this study, the impact of hydration status on dermal uptake of pesticides in two species of amphibians is examined. Absorption of pesticides in anurans occurs primarily through a highly vascularized dermal seat patch; however, pesticides can also enter through the superficial dermis following exposure. Despite the growing body of literature on dermal exposure in amphibians, little is known on how hydration status influences uptake. Thus, the objective of this study was to investigate the influence of hydration status on absorption of pesticides (atrazine, triadimefon, metolachlor, chlorothalonil, and imidacloprid) in southern leopard frogs (Lithobates sphenocephala) and Fowler's toads (Anaxyrus fowleri). Amphibian treatments included dehydration periods of 0, 2, 4, 6, 8, or 10 h prior to exposure to pesticide-contaminated soils for 8 h. Following exposure, soil and whole-body homogenates were extracted and analyzed by LC-MS/MS. Dehydration time was then regressed against post-exposure concentrations to infer the impact of dehydration on dermal pesticide uptake. Increased dehydration time resulted in significantly lowered pesticide concentrations in both species (F = 67.66, p = 0.007) for the five pesticides studied. This phenomenon could be due to an energy and/or dilution effect.

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Section: Discussionmentioning

confidence: 99%

Effect of hydration status on pesticide uptake in anurans following exposure to contaminated soils

Glinski

Henderson

Meter

et al. 2018

Environ Sci Pollut Res

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“…In the course of the analysis, two papers overlooked during the development of the original WoE assessment were identified and analyzed. In the end, the papers included in the QWoE update are: (Chang et al 2005;Hecker et al 2005;Svartz et al 2012;Ghodageri and Katti 2013;Olker 2014;Shenoy 2014;Baxter et al 2015;Chen et al 2015;Sai et al 2015Sai et al , 2018Syngenta 2015Syngenta , 2017aSyngenta , 2017bVan Der Kraak et al 2015;Wirbisky and Freeman 2015;dos Santos Mendonc¸a et al 2016;Ehrsam et al 2016;Gustafson et al 2016;Khan et al 2016aKhan et al , 2016bKhan et al , 2016cLiu et al 2016;Richter et al 2016;Russart and Rhen 2016;Tulaby Dezfuly and Mohammadian 2016;Wirbisky et al 2016aWirbisky et al , 2016bWirbisky et al , 2016cD'Angelo and Freeman 2017;Du Gas et al 2017;Hoskins et al 2017;Jones et al 2017;Khalil et al 2017;Kirsten et al 2017;McMahon et al 2017;Persch et al 2017;Scott et al 2017;Shukla et al 2017;Snyder et al 2017;Wang et al 2017;…”

Section: Methodsmentioning

confidence: 99%

“…The American toad (Anaxyrus americanus) and gray tree frog (Hyla versicolor) tadpoles were exposed to atrazine for 48-h at 10, 50, 250, or 1250 lg/L under laboratory conditions (Snyder et al 2017) (quality of methods for both experiments was 1.8). No mortality was observed in any of the tested concentrations for either species, making the score for relevance 0.…”

Section: Survival Of Fish and Amphibians After Short-term Exposures Tmentioning

confidence: 99%

“…The concentrations used in this study were environmentally unrealistic. Exposures to atrazine at a concentration of 1250 lg/L (but not 10, 50, and 250 mg/L) in tadpoles of the American toad (A. americanus) and H. versicolor for 48 h resulted in changes in metabolite profiles (Snyder et al 2017). Because they were observed at unrealistic concentrations and would be expected to result non-specific narcosis, they were judged to be of no relevance to apical endpoints.…”

Section: Physiological and Biochemical Effects In Vivomentioning

confidence: 99%

See 1 more Smart Citation

Effects of atrazine on fish, amphibians, and reptiles: update of the analysis based on quantitative weight of evidence

Hanson

Solomon

Kraak

et al. 2019

Critical Reviews in Toxicology

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Quantitative weight of evidence (QWoE) provides a framework and process for evaluating different toxicological studies based on quality and relevance of the results. This framework allows for data from these studies to be combined in separate lines of evidence to address causality, and relevance to environmental risks. In 2014, such a QWoE that examined the body of available company reports and peer reviewed literature regarding the effects of the herbicide atrazine on fish, amphibians, and reptiles was published. Since that time, new studies have been conducted and/or published. One of the advantages of the QWoE framework is that additional information can be added as it becomes available. Thus, these new studies were evaluated in the same manner as previously and the new data incorporated into the existing QWoE. As before, the new updated QWoE was based on the same process of objective scoring of individual studies with respect to the quality of the methods and the relevance of individual responses to the apical endpoints of survival, growth, development, and reproduction. These new data did not identify new responses or indicate any relevant effects of atrazine. The new updated QWoE analysis concluded that atrazine does not adversely affect fish, amphibians, and reptiles, at environmentally relevant concentrations (<100 mg atrazine/L), which is consistent with the previous conclusions. These new studies and data are discussed in this paper and the accompanying supplement information provides detailed and transparent information to support these conclusions.

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“…Metabolomics allows an investigation into the phenotypic response of individuals following chemical exposure and has been used successfully to profile the effects of environmental contaminants in humans (Bonvallot et al 2018) and ecological species (Lankadurai et al 2013). These approaches have been used to evaluate the biochemical perturbations following pesticide exposures in both larval and terrestrial phase amphibians (Snyder et al 2017;Glinski et al 2019;Van Meter et al 2019), as well as endocrine disruption associated with impacts on metamorphosis (Thambirajah et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Cross‐Taxa Distinctions in Mechanisms of Developmental Effects for Aquatic Species Exposed to Trifluralin

Awkerman

Lavelle

Henderson

et al. 2020

Enviro Toxic and Chemistry

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Standard ecological risk assessment practices often rely on larval and juvenile fish toxicity data as representative of the amphibian aquatic phase. Empirical evidence suggests that endpoints measured in fish early life stage tests are often sufficient to protect larval amphibians. However, the process of amphibian metamorphosis relies on endocrine cues that affect development and morphological restructuring and are not represented by these test endpoints. The present study compares developmental endpoints for zebrafish (Danio rerio) and the African clawed frog (Xenopus laevis), 2 standard test species, exposed to the herbicide trifluralin throughout the larval period. Danio rerio were more sensitive and demonstrated a reduction in growth measurements with increasing trifluralin exposure. Size of X. laevis at metamorphosis was not correlated with exposure concentration; however, time to metamorphosis was delayed relative to trifluralin concentration. Gene expression patterns indicate discrepancies in response by D. rerio and X. laevis, and dose‐dependent metabolic activity suggests that trifluralin exposure perturbed biological pathways differently within the 2 species. Although many metabolites were correlated with exposure concentration in D. rerio, nontargeted hepatic metabolomics identified a subset of metabolites that exhibited a nonmonotonic response to trifluralin exposure in X. laevis. Linking taxonomic distinctions in cellular‐level response with ecologically relevant endpoints will refine assumptions used in interspecies extrapolation of standard test effects and improve assessment of sublethal impacts on amphibian populations. Environ Toxicol Chem 2020;39:1797–1812. Published 2020. This article is a US government work and is in the public domain in the USA.

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Biomarker analysis of American toad (Anaxyrus americanus) and grey tree frog (Hyla versicolor) tadpoles following exposure to atrazine

Cited by 19 publications

References 51 publications

Effect of hydration status on pesticide uptake in anurans following exposure to contaminated soils

Effect of hydration status on pesticide uptake in anurans following exposure to contaminated soils

Effects of atrazine on fish, amphibians, and reptiles: update of the analysis based on quantitative weight of evidence

Cross‐Taxa Distinctions in Mechanisms of Developmental Effects for Aquatic Species Exposed to Trifluralin

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