Biomanufacturing Seamless Tubular and Hollow Collagen Scaffolds with Unique Design Features and Biomechanical Properties

Singh, Anirudha; Lee, David; Sopko, Nikolai A.; Matsui, Hotaka; Sabnekar, Praveena; Liu, Xiaopu; Elisseeff, Jennifer H.; Schoenberg, Mark; Pienta, Kenneth J.; Bivalacqua, Trinity J.

doi:10.1002/adhm.201601136

Cited by 17 publications

(7 citation statements)

References 44 publications

(47 reference statements)

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“…436 From the neural perspective, wall morphology has an impact on the material's resistance. 425 with the permission of Elsevier: Aiii, 426 Ciii, 427 4iv; 428 with the permission of RSC: Ai; 70 and with the permission of Wiley: Aii, 421 Bi, 429 B: ii and iii, 430,431 Ci, 431 Cii, 432 D:iv and v. 433 Abbreviations: HUVEC: human umbilical vein endothelial cells; MSCs: mesenchymal cells; PCL: poly(e-caprolactone); PG2: pullulan/gelatin; P4HB-PGA: poly(4-hydroxybutyrate)-polyglycolide; rBC/Gel: bacterial cellulose-gelatin; RP: radial pore-oriented; SEM: scanning electron microscopy; TE: tissue engineering; VSCM: volume-stable collagen matrix.…”

Section: Reconstructive Tissue Engineeringmentioning

confidence: 99%

“…20Bi and ii, hollow collagen hyaluronic with platelet-rich plasma doped constructs can be tailored for use as ureter-like tubular and bladder-like scaffolds due to the natural collagen's chemical composition and the scaffold's ability to withstand fluid pressures. 430,437 In a similar context, abdominal tissue regeneration has also taken advantage of porous solutions. A radial poreoriented collagen scaffold (RP-composite) was tested in rabbit diaphragmatic hernias.…”

Section: Reconstructive Tissue Engineeringmentioning

confidence: 99%

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Porous biomaterials for tissue engineering: a review

et al. 2022

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Section: Reconstructive Tissue Engineeringmentioning

confidence: 99%

Section: Reconstructive Tissue Engineeringmentioning

confidence: 99%

Porous biomaterials for tissue engineering: a review

et al. 2022

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“…Bone marrow mesenchymal stem cells (BMSCs) are a type of pluripotent stem cells originating from the mesoderm. They are able to differentiate into bone, cartilage and fat (17). Autologous bone marrow transplantation can avoid the complications caused by transplant rejection, but research on and application of this technique are costly (18).…”

Section: Introductionmentioning

confidence: 99%

Comparison of the therapeutic effects of adipose‑derived and bone marrow mesenchymal stem cells on erectile dysfunction in diabetic rats

Chen

Zhu²,

Wang

et al. 2019

Int J Mol Med

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The aim of the present study was to compare the effects of adipose-derived mesenchymal stem cell (ADSC) and bone marrow mesenchymal stem cell (BMSC) transplantation into the corpora cavernosa of diabetic rats with erectile function. ADSCs and BMSCs were isolated and identified by flow cytometry. Rats with streptozocin-induced diabetes were screened using apomorphine to obtain a rat model of diabetic erectile dysfunction, followed by transplantation of ADSCs and BMSCs into the corpora cavernosa. Two weeks later, the rats were again injected with apomorphine, the intracavernous pressure (ICP) and mean arterial pressure (MAP) of the penile tissue were measured, and the corpus cavernosum tissues were harvested. Angiogenic endothelial nitric oxide synthase (eNOS) expression was detected by western blotting and immunofluorescence analysis. The blood vessels in the corpus cavernosum were observed following hematoxylin and eosin (H&E) staining, and the expression of collagen was detected by Sirius Red staining. The cellular ultrastructure was examined by transmission electron microscopy. Intracavernous injection of ADSCs significantly increased ICP and ICP/MAP. Western blotting and immunofluorescence results revealed that ADSC treatment improved the expression of eNOS in the penile tissue of diabetic rats. The H&E staining results demonstrated that ADSC treatment promoted revascularization of the corpus cavernosum, and the results of Sirius Red staining revealed that ADSC treatment reduced penile collagen in diabetic rats. Transmission electron microscopy examination revealed that the ultrastructure of the tissues in the ADSC-treated group was more complete compared with that in the untreated diabetic model group. In conclusion, ADSCs were found to be more effective compared with BMSCs in treating diabetes-related erectile dysfunction.

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“…Collagen scaffolds were fabricated in a custom-made balloon (transparent latex based) chamber as described earlier. 51 In brief, collagen scaffolds were prepared by neutralizing type I collagen (5 mg/mL in HCl, 10 mL) with 8.8 mL of Dulbecco's modified Eagle's medium (DMEM) with 1.0 g/L D-Glucose, L-glutamate, 110 mg/L sodium pyruvate (Life Technologies, NY), 1.0 mL of fetal bovine serum (FBS), and 0.2 mL of HEPES (×1, 1M) solution at 4 °C. (A) Collagen discs: The setup for the collagen disc was made with two customized molds in a hollow and cylindrical-shape balloon chamber.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Aliphatic Chain Modification of Collagen Type I: Development of Elastomeric, Compliant, and Suturable Scaffolds

Sharma

Chen

et al. 2020

ACS Appl. Bio Mater.

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Collagen type I is one of the most suitable natural biomaterials for constructing tissue-engineering scaffolds. Despite their biocompositional similarities to physiological tissues, these scaffolds lack host specific and matching mechanical properties. While it is possible to enhance their stiffness by cross-linking, it often compromises their abilities to expand or strain under minimal stress, that is, compliance (inverse of stiffness). Here, we report a simple, inexpensive, cross-linking-and elastinfree collagen-based material composition for developing elastomeric scaffolds that are highly compliant, soft yet strong, and suturable, therefore, clinically attractive. Our strategy utilizes room-temperature modification of collagen type I scaffolds with linear aliphatic chains of various lengths (C7−C18). In particular, dodecenylsuccinic anhydride (size: C12, DDSA) modified scaffolds elongated up to 400% of its initial length compared to only ∼20% for collagen-control within the applied tensile stress of 0.2 MPa without breaking. Furthermore, the suture retention strength value increased to 60 g-force from 30 g-force for collagen control. We confirmed that the C12-modified material remained structurally stable at the physiological temperature (37 °C) with a tan δ value of ∼0.3, similar to collagen control; however, tan δ increased sharply for C12-modified collagen above 42 °C, compared to 59 °C for collagen control. To understand the mechanism of hyperextensibility, we studied the morphology of the resultant material by transmission electron microscopy (TEM), which showed an altered microstructure of C12-modified collagen scaffolds. While the partially C12-modified sample had a mixture of typical collagen type I triple helix and diffused gelatinized random coil-like configuration, the fully modified samples showed thick wrinkled and entangled ribbon-like microstructures, which was different than that of thermally denatured gelatin. We further confirmed that the resultant material allowed cell growth in vitro and in vivo in a subcutaneous mouse model.

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Biomanufacturing Seamless Tubular and Hollow Collagen Scaffolds with Unique Design Features and Biomechanical Properties

Cited by 17 publications

References 44 publications

Porous biomaterials for tissue engineering: a review

Porous biomaterials for tissue engineering: a review

Comparison of the therapeutic effects of adipose‑derived and bone marrow mesenchymal stem cells on erectile dysfunction in diabetic rats

Aliphatic Chain Modification of Collagen Type I: Development of Elastomeric, Compliant, and Suturable Scaffolds

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