Bioluminescent reporters for identification of gene allelic variants

Krasitskaya, Vasilisa V.; Burakova, Ludmila P.; Pyshnaya, I. A.; Frank, Ludmila A.

doi:10.1134/s1068162012030090

Cited by 9 publications

(8 citation statements)

References 14 publications

(15 reference statements)

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“…Additionally, one of the simplest enzymatic bioassay systems, i.e., a Coelenteramide-containing Fluorescent Protein (CLM-FP), was used to evaluate the activation bioeffect of tritium. CLM-FPs are products of bioluminescent reactions of coelenterates that constitute a potential use in biomedicine as biomarkers [36][37][38]. The fluorescence characteristics of CLM-FPs were intensively studied [39][40][41][42][43][44][45][46]; they were found to be sensitive to external exposures: excitation energy [44], chemical agents [47,48], and radioactivity [49,50].…”

Section: ⎯⎯mentioning

confidence: 99%

Enzymatic Responses to Low-Intensity Radiation of Tritium

Rozhko

Nemtseva

Gardt

et al. 2020

IJMS

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The present study considers a possible role of enzymatic reactions in the adaptive response of cells to the beta-emitting radionuclide tritium under conditions of low-dose exposures. Effects of tritiated water (HTO) on the reactions of bacterial luciferase and NAD(P)H:FMN-oxidoreductase, as well as a coupled system of these two reactions, were studied at radioactivity concentrations ≤ 200 MBq/L. Additionally, one of the simplest enzymatic reactions, photobiochemical proton transfer in Coelenteramide-containing Fluorescent Protein (CLM-FP), was also investigated. We found that HTO increased the activity of NAD(P)H:FMN-oxidoreductase at the initial stage of its reaction (by up to 230%); however, a rise of luciferase activity was moderate (<20%). The CLM-FP samples did not show any increase in the rate of the photobiochemical proton transfer under the exposure to HTO. The responses of the enzyme systems were compared to the ‘hormetic’ response of luminous marine bacterial cells studied earlier. We conclude that (1) the oxidoreductase reaction contributes significantly to the activation of the coupled enzyme system and bacterial cells by tritium, and (2) an increase in the organization level of biological systems promotes the hormesis phenomenon.

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Section: ⎯⎯mentioning

confidence: 99%

Enzymatic Responses to Low-Intensity Radiation of Tritium

Rozhko

Nemtseva

Gardt

et al. 2020

IJMS

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“…This is a basis for medico-biological application of the photoprotein bioluminescence in different types of diagnostics: for monitoring the Ca 2 + content, protein location in cells and tissues, and others. Additionally, bioluminescence of photoproteins is used as an intracellular marker [15,16]. These are reasons for intensive investigations of the photoprotein bioluminescence mechanism.…”

Section: Introductionmentioning

confidence: 99%

Ultraviolet fluorescence of coelenteramide and coelenteramide-containing fluorescent proteins. Experimental and theoretical study

Alieva

Tomilin

Кузубов

et al. 2016

Journal of Photochemistry and Photobiology B: Biology

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Coelenteramide-containing fluorescent proteins are products of bioluminescent reactions of marine coelenterates. They are called 'discharged photoproteins'. Their light-induced fluorescence spectra are variable, depending considerably on external conditions. Current work studies a dependence of light-induced fluorescence spectra of discharged photoproteins obelin, aequorin, and clytin on excitation energy. It was demonstrated that photoexcitation to the upper electron-excited states (260-300nm) of the discharged photoproteins initiates a fluorescence peak in the near UV region, in addition to the blue-green emission. To characterize the UV fluorescence, the light-induced fluorescence spectra of coelenteramide (CLM), fluorophore of the discharged photoproteins, were studied in methanol solution. Similar to photoproteins, the CLM spectra depended on photoexcitation energy; the additional peak (330nm) in the near UV region was observed in CLM fluorescence at higher excitation energy (260-300nm). Quantum chemical calculations by time depending method with B3LYP/cc-pVDZ showed that the conjugated pyrazine-phenolic fragment and benzene moiety of CLM molecule are responsible for the additional UV fluorescence peak. Quantum yields of CLM fluorescence in methanol were 0.028±0.005 at 270-340nm photoexcitation. A conclusion was made that the UV emission of CLM might contribute to the UV fluorescence of the discharged photoproteins. The study develops knowledge on internal energy transfer in biological structures - complexes of proteins with low-weight aromatic molecules.

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“…see ref. [8][9][10]. We see obvious reasons for applying this technique in any dual assay regardless of its type -immune, hybridization or both.…”

Section: Resultsmentioning

confidence: 99%