Bioluminescent bioreporter integrated circuits: potentially small, rugged and inexpensive whole-cell biosensors for remote environmental monitoring

Nivens, David E.; McKnight, Timothy E.; Moser, S.; Osbourn, S.J.; Simpson, Michael L.; Sayler, Gary S.

doi:10.1046/j.1365-2672.2003.02114.x

Cited by 108 publications

(57 citation statements)

References 83 publications

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“…These organisms may be used for environmental monitoring (286,288,401), as well as to produce N-acyl homoserine lactone autoinducer molecules for controlling infections and biofilm formation (373). Vibrios are also used in vaccine (207) and probiotic (411) production.…”

Section: Perspectives and Exploitationmentioning

confidence: 99%

Biodiversity of Vibrios

Thompson

Iida

Swings

2004

Microbiol Mol Biol Rev

1,062

888

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Vibrios are ubiquitous and abundant in the aquatic environment. A high abundance of vibrios is also detected in tissues and/or organs of various marine algae and animals, e.g., abalones, bivalves, corals, fish, shrimp, sponges, squid, and zooplankton. Vibrios harbour a wealth of diverse genomes as revealed by different genomic techniques including amplified fragment length polymorphism, multilocus sequence typing, repetetive extragenic palindrome PCR, ribotyping, and whole-genome sequencing. The 74 species of this group are distributed among four different families, i.e., Enterovibrionaceae, Photobacteriaceae, Salinivibrionaceae, and Vibrionaceae. Two new genera, i.e., Enterovibrio norvegicus and Grimontia hollisae, and 20 novel species, i.e., Enterovibrio coralii, Photobacterium eurosenbergii, V. brasiliensis, V. chagasii, V. coralliillyticus, V. crassostreae, V. fortis, V. gallicus, V. hepatarius, V. hispanicus, V. kanaloaei, V. neonatus, V. neptunius, V. pomeroyi, V. pacinii, V. rotiferianus, V. superstes, V. tasmaniensis, V. ezurae, and V. xuii, have been described in the last few years. Comparative genome analyses have already revealed a variety of genomic events, including mutations, chromosomal rearrangements, loss of genes by decay or deletion, and gene acquisitions through duplication or horizontal transfer (e.g., in the acquisition of bacteriophages, pathogenicity islands, and super-integrons), that are probably important driving forces in the evolution and speciation of vibrios. Whole-genome sequencing and comparative genomics through the application of, e.g., microarrays will facilitate the investigation of the gene repertoire at the species level. Based on such new genomic information, the taxonomy and the species concept for vibrios will be reviewed in the next years

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Section: Perspectives and Exploitationmentioning

confidence: 99%

Biodiversity of Vibrios

Thompson

Iida

Swings

2004

Microbiol Mol Biol Rev

1,062

888

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“…We mainly employ GFP as a quantifiable biological response, but also demonstrate that the design strategy can be used to control a natural phenotype by creating a strain (A2) that enters a biofilm-forming state in response to transient activation of the SOS pathway. Although the engineering of cellular behavior is not novel, most existing programmed cells are designed for specific purposes, such as whole-cell biosensing (35)(36)(37)(38)(39), programmed self-destruction (40)(41)(42)(43), and protein synthesis controlled by excess glucolytic flux (44) or cell density (33,45). The modular approach that we propose would facilitate a standardization of genetic circuits that, in analogy to electronic circuit modules, could be used as general components in the construction of programmable cells for a variety of biotechnological and bioengineering applications.…”

mentioning

confidence: 99%

Programmable cells: Interfacing natural and engineered gene networks

Kobayashi

Kærn

Araki

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

537

493

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Novel cellular behaviors and characteristics can be obtained by coupling engineered gene networks to the cell's natural regulatory circuitry through appropriately designed input and output interfaces. Here, we demonstrate how an engineered genetic circuit can be used to construct cells that respond to biological signals in a predetermined and programmable fashion. We employ a modular design strategy to create Escherichia coli strains where a genetic toggle switch is interfaced with: (i) the SOS signaling pathway responding to DNA damage, and (ii) a transgenic quorum sensing signaling pathway from Vibrio fischeri. The genetic toggle switch endows these strains with binary response dynamics and an epigenetic inheritance that supports a persistent phenotypic alteration in response to transient signals. These features are exploited to engineer cells that form biofilms in response to DNA-damaging agents and cells that activate protein synthesis when the cell population reaches a critical density. Our work represents a step toward the development of ''plug-and-play'' genetic circuitry that can be used to create cells with programmable behaviors.heterologous gene expression ͉ synthetic biology ͉ Escherichia coli

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“…The bioluminescent-bioreporter integrated circuit (BBIC) was one of the first developments in which genetically engineered bacteria are interfaced with an electronic circuit and has been reported many times in the last decade since its first development [Simpson et al 2001b;Bolton et al 2002;Ripp et al 2003;Nivens et al 2004;Vijayaraghavan et al 2007]. In these studies, a biosensor, i.e.…”

Section: Optical Microchip-based Biosensorsmentioning

confidence: 99%

Synthetic Biology and Microdevices

Venken

Marchal

Vanderleyden

2013

J. Emerg. Technol. Comput. Syst.

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Recent developments demonstrate that the combination of microbiology with micro-and nanoelectronics is a successful approach to develop new miniaturized sensing devices and other technologies. In the last decade, there is a shift from the optimization of the abiotic components, e.g. the chip, to the improvement of the processing capabilities of cells through genetic engineering. The synthetic biology approach will not only give rise to systems with new functionalities, but will also improve the robustness and speed of their response towards applied signals. To this end, the development of new genetic circuits has to be guided by computational design methods that enable to tune and optimize the circuit response. As the successful design of genetic circuits is highly dependent on the quality and reliability of its composing elements, intense characterization of standard biological parts will be crucial for an efficient rational design process in the development of new genetic circuits. Microengineered devices can thereby offer a new analytical approach for the study of complex biological parts and systems. By summarizing the recent techniques in creating new synthetic circuits and in integrating biology with microdevices, this review aims at emphasizing the power of combining synthetic biology with microfluidics and microelectronics.

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Bioluminescent bioreporter integrated circuits: potentially small, rugged and inexpensive whole-cell biosensors for remote environmental monitoring

Cited by 108 publications

References 83 publications

Biodiversity of Vibrios

Biodiversity of Vibrios

Programmable cells: Interfacing natural and engineered gene networks

Synthetic Biology and Microdevices

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