Bioluminescent assay of microbial ATP in postmortem tissues for the estimation of postmortem interval

Abstract

To study the relationship between changes of microbial ATP in four kinds of murine tissues and the postmortem interval (PMI), healthy SD rats were sacrificed and their muscles, livers, spleens and kidneys were sampled at different postmortem intervals. The concentration of microbial ATP was detected using bioluminescent assay and the data was statistically analyzed. The concentration of microbial ATP in muscle increased with PMI time. The peak appeared at the 7th day after death, and at the 10th day, microbial ATP in muscle tissue increased again. In internal organs, the peaks of microbial ATP were observed at the 8th day after death and the level decreased during 8-10 d. The differences in microbial ATP concentration in liver, spleen and kidney were not statistically significant. During day 0 to day 9 after death, the correlation was best between PMI and microbial ATP in muscle. With PMI as the independent variable, the cubic polynomial regression equation was Y=0.02X(3)-0.166X(2)-0.666X+13.412 (R (2)=0.989, P<0.01). In internal organs, the best correlation was found between PMI and microbial ATP during day 0 to day 10. With PMI as the independent variable, the cubic polynomial regression equation was Y=0.016X(3)-0.127X(2)-0.809X+13.324 (R (2)=0.986, P<0.01). There existed high correlations between PMI and microbial ATP concentration in rat tissues. Since only a small amount of tissue was needed for the detection and the sample was not affected by self-decomposition, the method may extend the time range of PMI estimation.

Characterization of protein in old myocardial infarction by FTIR micro-spectroscopy

“…Other methods could also be used to assess the molecular changes of muscles or OMI marker [24,25] , but they all consumed much time. Taking into consideration of results of the present study, we suggest that the substructure differences revealed from the FTIR spectral intervals that present a discrimination potential between protein analyses should be considered in the identification of amide types for analyzing infarct scar formation and assembly in histological sections.…”

Estimation of the postmortem interval based on the human decomposition process

“…Therefore, other methods can be used to estimate the PMI for human remains found on land, for example Megyesi's Total Body Score (TBS), 2 Gelderman's Total Decomposition Score (TDS), 29 entomology, 5,[30][31][32] volatile fatty acids in soil 33,34 and ATP (Adenosine Triphosphate) determination from the brain, liver, spleen, kidney and muscle. [35][36][37] The former two methods are decomposition scoring methods which describe the decomposition process. With these methods, the PMI can be estimated using the accumulated degree days (ADD).…”

Numerical Simulation the Airflow Field in the Flame Retardant Cloth Ducts

“…In 2007, Nielsen stated that a relatively even temperature and low air flow velocity in room was controlled by FADS [5]. In 2009, Liu Chao used the FLUENT software to simulate the air distribution of FADS and the ordinary air conditioning system [6]. In 2011, Chen Fu-jiang studied FADS by numerical simulation and experimental.…”