Bioluminescence of Vibrio fischeri: bacteria respond quickly and sensitively to pulsed microwave electric (but not magnetic) fields

Williams, Catrin H.; Geroni, Gilles M.; Lloyd, David; Choi, Heungjae; Clark, Nicholas; Pirog, Antoine; Lees, Jonathan; Porch, Adrian

doi:10.1117/1.jbo.24.5.051412

Cited by 10 publications

(5 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Briefly, to the bacterial suspension, the diluent (2% aqueous NaCl) was added, immediately followed by the addition of film fragments of the same size. The test was performed using Microtox M500 with Modern Water Microtox Omni 4.2 software [ 118 ]. The analysis was conducted in duplicate.…”

Section: Methodsmentioning

confidence: 99%

Chitosan-Gelatin Films Cross-Linked with Dialdehyde Cellulose Nanocrystals as Potential Materials for Wound Dressings

Węgrzynowska-Drzymalska

Mlynarczyk

Chełminiak-Dudkiewicz

et al. 2022

IJMS

View full text Add to dashboard Cite

In this study, thin chitosan-gelatin biofilms cross-linked with dialdehyde cellulose nanocrystals for dressing materials were received. Two types of dialdehyde cellulose nanocrystals from fiber (DNCL) and microcrystalline cellulose (DAMC) were obtained by periodate oxidation. An ATR-FTIR analysis confirmed the selective oxidation of cellulose nanocrystals with the creation of a carbonyl group at 1724 cm−1. A higher degree of cross-linking was obtained in chitosan-gelatin biofilms with DNCL than with DAMC. An increasing amount of added cross-linkers resulted in a decrease in the apparent density value. The chitosan-gelatin biofilms cross-linked with DNCL exhibited a higher value of roughness parameters and antioxidant activity compared with materials cross-linked with DAMC. The cross-linking process improved the oxygen permeability and anti-inflammatory properties of both measurement series. Two samples cross-linked with DNCL achieved an ideal water vapor transition rate for wound dressings, CS-Gel with 10% and 15% addition of DNCL—8.60 and 9.60 mg/cm2/h, respectively. The swelling ability and interaction with human serum albumin (HSA) were improved for biofilms cross-linked with DAMC and DNCL. Significantly, the films cross-linked with DAMC were characterized by lower toxicity. These results confirmed that chitosan-gelatin biofilms cross-linked with DNCL and DAMC had improved properties for possible use in wound dressings.

show abstract

Section: Methodsmentioning

confidence: 99%

Chitosan-Gelatin Films Cross-Linked with Dialdehyde Cellulose Nanocrystals as Potential Materials for Wound Dressings

Węgrzynowska-Drzymalska

Mlynarczyk

Chełminiak-Dudkiewicz

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…To evaluate the toxicities of the films precooled diluent (supplied by the producer) was added to the cuvettes containing Aliivibrio fischeri bacteria suspension, which was followed immediately by immersing of the film fragment. The decrease of cell viability was measured with Microtox ® M500 and Microtox Omni software (version–4.2, Modern Water, Londyn, UK) based on the cell bioluminescence at 490 nm [ 54 ].…”

Section: Methodsmentioning

confidence: 99%

Crosslinking of Chitosan with Dialdehyde Chitosan as a New Approach for Biomedical Applications

Węgrzynowska-Drzymalska

Grebicka

Mlynarczyk

et al. 2020

Materials

View full text Add to dashboard Cite

Materials based on natural high molecular compounds are particularly interesting for biomedical applications. It is known that the cross-linking agent used for preparation of biomacromolecule-based materials is as important as used biopolymer. Therefore, natural cross-linkers containing reactive carbonyl groups are of great interest especially for modifying properties of natural polysaccharides. One of the most popular cross-linking agents is glutaraldehyde. Nevertheless, the unreacted particles can be released from the cross-linked material and cause cytotoxic effects. This can be eliminated when using a cross-linker based e.g., on polysaccharides. This article describes quick and efficient synthesis of dialdehyde chitosan (DACS) and its application for the preparation of chitosan films. Materials obtained with different amount of DACS were fully characterized in terms of structure and surface morphology. Thermal and mechanical properties as well as hydrophilic character were also examined. The results obtained were compared with the materials obtained by cross-linking chitosan with low molecular weight glutaraldehyde and high molecular weight cross-linking agent based on polysaccharide–dialdehyde starch. Toxicity of all obtained materials was tested using the Microtox® test. It has been shown that due to better mechanical, thermal and surface properties as well as lower toxicity, dialdehyde chitosan is a very promising crosslinking agent.

show abstract

“…Notably, the optimum temperature for luciferase was 25°C. As luciferase is temperature dependent, the bioluminescence exhibited higher intensity at the range of 25 to 30°C and fluctuated notoriously above 30°C as mentioned by [38][39][40]. Also, it is noted that the bioluminescence stability of Vibrio sp.…”

Section: Discussionmentioning

confidence: 88%

Toxicity monitoring of solvents, hydrocarbons, and heavy metals using statistically optimized model of luminous Vibrio sp. 6HFE

Hassan

Eltarahony

Abu-Elreesh

et al. 2022

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

Background The utilization of bioluminescent bacteria in environmental monitoring of water contaminates considers being a vital and powerful approach. This study aimed to isolate, optimize, and apply luminescent bacteria for toxicity monitoring of various toxicants in wastewater. Results On the basis of light intensity, strain Vibrio sp. 6HFE was initially selected, physiologically/morphologically characterized, and identified using the 16SrDNA gene. The luminescence production was further optimized by employing statistical approaches (Plackett-Burman design and central composite design). The maximum bioluminescence intensity recorded 1.53 × 106 CPS using optimized medium containing (g/L), yeast extract (0.2g), CaCl2 (4.0), MgSO4 (0.1), and K2HPO4 (0.1) by 2.3-fold increase within 1h. The harnessing of Vibrio sp. 6HFE as a bioluminescent reporter for toxicity of organic solvents was examined using a bioluminescence inhibition assay. According to IC50 results, the toxicity order of such pollutants was chloroform > isoamyl > acetic acid > formamide > ethyl acetate > acetonitrile > DMSO > acetone > methanol. However, among eight heavy metals tested, the bioluminescence was most sensitive to Ag+ and Hg+ and least sensitive to Co2+ and Ni2+. Additionally, the bioluminescence was inhibited by benzene, catechol, phenol, and penta-chlorophenol at 443.1, 500, 535.1, and 537.4 ppm. Conclusion Vibrio sp. 6HFE succeeded in pollution detection at four different environmental and wastewater samples revealing its efficiency in ecotoxicity monitoring.

show abstract

Bioluminescence of Vibrio fischeri: bacteria respond quickly and sensitively to pulsed microwave electric (but not magnetic) fields

Cited by 10 publications

References 43 publications

Chitosan-Gelatin Films Cross-Linked with Dialdehyde Cellulose Nanocrystals as Potential Materials for Wound Dressings

Chitosan-Gelatin Films Cross-Linked with Dialdehyde Cellulose Nanocrystals as Potential Materials for Wound Dressings

Crosslinking of Chitosan with Dialdehyde Chitosan as a New Approach for Biomedical Applications

Toxicity monitoring of solvents, hydrocarbons, and heavy metals using statistically optimized model of luminous Vibrio sp. 6HFE

Contact Info

Product

Resources

About