Bioluminescence Image as a Quantitative Imaging Biomarker for Preclinical Evaluation of Cryoablation in a Murine Model

Lamid-Ochir, Oyunbold; Nakajima, Takahito; Miyazaki, Masaru; Zhang, Xieyi; Erdene, Khongorzul; Murakami, Takashi; Tsushima, Yoshito

doi:10.1016/j.jvir.2018.03.013

Cited by 5 publications

(4 citation statements)

References 40 publications

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“…There was a previous study on cryoablation using BLI for treatment evaluation and diagnostic imaging both in vitro and in vivo. 27 In our study, posttreatment cellular death may have occurred via an indirect cellular injury, or the failure of microcirculation due to edema after thawing. 28 This, in turn needs to be compared to the results of a direct cellular injury mechanism by cryogenic necrosis after ice crystal formation in an in vivo environment.…”

Section: Discussionmentioning

confidence: 69%

Evaluation of Various Cryoablation Protocols in Multiple Cell 1ines using Quantitative Biomarkers of Bioluminescence Imaging: An <i>in vitro</i> Study

Khurelbaatar,

Kartamihardja,

Miyazaki

et al. 2024

kmj

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Background & Aims: To investigate whether the optimal treatment protocols of cryoablation differ among various cell lines utilizing bioluminescence imaging (BLI). Methods: Eight luciferase-expressing cancer cell lines originated from various organs were used in this study, including lung, renal, liver, breast, colon, prostate, and skin. These cell lines were uniformly seeded in 96-well plates. The cell lines were then divided into four groups: Group 0 (G0), as a control group, and three treatment groups (G1-G3). G1 -G3 were subjected to cryoablation at temperatures of -45℃, -60℃, and -80℃, respectively, with varying treatment cycles (single, double, or triple cycle, respectively). Each freeze-thaw cycle consisted of ten minutes of freezing followed by five minutes of thawing. Finally, the average radiance of the cells was assessed using BLI.Results: Cryoablation at "-60℃ and -80℃" showed a significant decrease in average radiance, even after a single cycle of treatment in all cell lines. The average radiance of cells treated with -45℃ was substantially lower than that of untreated cell lines except melanoma. Conclusion: We found that -45℃ successfully eradicated all cancer cells across multiple cycles except melanoma. The most effective cryoablation protocol for all cell lines was observed at temperatures of -60℃ or lower.

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Section: Discussionmentioning

confidence: 69%

Evaluation of Various Cryoablation Protocols in Multiple Cell 1ines using Quantitative Biomarkers of Bioluminescence Imaging: An <i>in vitro</i> Study

Khurelbaatar,

Kartamihardja,

Miyazaki

et al. 2024

kmj

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“…Bioluminescence imaging (BLI) based on the sensitive detection of visible light produced by luciferase has been developed over 20 years as a tool for molecular imaging [15]. The advantage of BLI is that it can provide biological information to monitor tumor growth and metastasis [16,17]. Multiple measurements can be made in the same animal over time by BLI [18].…”

Section: Bioluminescence Imagingmentioning

confidence: 99%

Imaging modalities for monitoring acute therapeutic effects after near‐infrared photoimmunotherapy in vivo

Zhang

Nakajima

Mizoi

et al. 2021

Journal of Biophotonics

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Near-infrared photoimmunotherapy (NIR-PIT) induces immediate cell death after irradiation with near-infrared (NIR) light.Acute therapeutic effects caused by NIR-PIT before the change of tumor size is essential to be monitored by imaging modalities. We summarized and compared the imaging modalities for evaluating acute therapeutic effects after NIR-PIT, and aimed to provide a better understanding of advantages and disadvantages of each modality for evaluation in clinical applications. Fluorescence imaging and fluorescence lifetime, with high resolution, remains high accumulation of fluorescence dyes in the normal organs. High resolution and noninvasiveness are the major advantages of magnetic resonance imaging, while 18 F-fluorodeoxyglucose positron emission tomography provides information about the glucose metabolism. Optical coherence tomography provided more information about the blood vessels. Thus, all of the imaging modalities play an important role in evaluating acute therapeutic effects after NIR-PIT.Clinicians should choose suitable modality according to specific purpose and conditions in clinical application.

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Section: Introductionmentioning

confidence: 99%

“…6 In particular, bioluminescence imaging (BLI), an optical molecular imaging modality that allows highly sensitive and quantitative detection of target tissue in animal models, has become a popular strategy for the real-time analysis of dynamic biological processes. 7 In AMI, damage to the bowel wall occurs because of hypoxia resulting from the interruption of blood flow in the mesenteric vessels. 3 These hypoxic and anoxic microenvironments may be targeted by obligatory or facultative anaerobic bacteria such as Salmonella typhimurium that are defective in the synthesis of ppGpp (DppGpp S. typhimurium), as reported in a previous study 8 that demonstrated the selective accumulation of the bacteria in the infarcted myocardium.…”

Section: Introductionmentioning

confidence: 99%

In Vivo Bioluminescence Imaging for Targeting Acute Hypoxic/Ischemic Small Intestine with Engineered Salmonella typhimurium

Moon

Zheng

Min

et al. 2020

Molecular Therapy - Methods & Clinical Development

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This study aimed at investigating the feasibility of bioluminescence imaging (BLI) with engineered Salmonella typhimurium (ΔppGpp S. typhimurium ) for visualizing acute hypoxic/ischemic bowels. At the start of 12- or 24-h reperfusion, ΔppGpp S. typhimurium was injected into the lateral tail veins of rats in which three segments of the small intestine were respectively subjected to 2, 3, and 4 h of ischemia. BLI and magnetic resonance imaging were performed at each reperfusion time point. Bioluminescence was exclusively detected in the hypoxic/ischemic segment of the intestine, showing the ability of ΔppGpp S. typhimurium to specifically target and proliferate in a hypoxic/ischemic area. Serial monitoring of these rat models revealed a progressive increase in bacterial bioluminescence in the ischemic intestines in conjunction with viable bacterial counts. The viable bacterial counts were positively correlated with lactate dehydrogenase levels after 24 h of reperfusion following 3 or 4 h of ischemia as well as interleukin-6 levels after 24 h of reperfusion following 4 h of ischemia. Our findings demonstrated that BLI was able to detect the acute hypoxic/ischemic bowel via monitoring of the distribution, internalization, and activity of administered ΔppGpp S. typhimurium . These findings may be useful for the early diagnosis of ischemic bowel disease.

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Bioluminescence Image as a Quantitative Imaging Biomarker for Preclinical Evaluation of Cryoablation in a Murine Model

Cited by 5 publications

References 40 publications

Evaluation of Various Cryoablation Protocols in Multiple Cell 1ines using Quantitative Biomarkers of Bioluminescence Imaging: An <i>in vitro</i> Study

Evaluation of Various Cryoablation Protocols in Multiple Cell 1ines using Quantitative Biomarkers of Bioluminescence Imaging: An <i>in vitro</i> Study

Imaging modalities for monitoring acute therapeutic effects after near‐infrared photoimmunotherapy in vivo

In Vivo Bioluminescence Imaging for Targeting Acute Hypoxic/Ischemic Small Intestine with Engineered Salmonella typhimurium

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