Bioluminescence Detection of Superoxide Anion Using Aequorin

Rahmani, Hossein; Ghavamipour, Fahimeh; Sajedi, Reza H.

doi:10.1021/acs.analchem.9b02293

Cited by 16 publications

(7 citation statements)

References 46 publications

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“…Hydroxyl radical is a very active reactive oxygen species, which could damage the structure of biological macromolecules and cause tissue damage or cell death ( Liu et al, 2010 ). Superoxide anion is produced during normal cellular respiration and plays fundamental roles in cellular physiology with its dysregulation being associated with a variety of diseases ( Rahmani, Ghavamipour & Sajedi, 2019 ). The ABTS radical assay is among the most abundant assays to evaluate antioxidant capacity ( Garcia-Leis et al, 2016 ).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of a strawberry fermented beverage with potential health benefits

Zhao

Wu²,

Chen

et al. 2021

PeerJ

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Background Functional fermented beverages are popular worldwide due to their potential to promote health. Starter culture is the main determinant of the final quality and flavor of fermented beverages. The co-cultivation of lactic acid bacteria (LAB) and yeast makes a significant contribution to the safe flavor of fermented beverages. However, the research on the potential of antioxidant, antimicrobial, and anti-biofilm formation of strawberry fermented beverage obtained by combining the LAB and yeast as starter cultures has not been well explored. Methods In this study, LAB and yeast were combined as starter culture to obtain strawberry fermented beverage. Fourier transform infrared (FTIR ) spectroscopy was used for the qualitative analysis of the fresh strawberry juice and fermented beverage. From the changes in antioxidant content, free radical scavenging ability, total superoxide dismutase (T-SOD) activity and total antioxidant capacity (T-AOC) to evaluate the antioxidant capacity of fermented beverage in vitro. The antibacterial ability was tested by the Oxford cup method. The biofilms of Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 6538 under fermented beverages treatment was observed by Fluorescence microscope. In addition, sensory analysis was conducted in this study. Results In this study, the absorption peaks of Fourier transform infrared between 1,542 cm−1 and 976 cm−1, suggest the existence of organic acids, sugars and ethanol. The total phenols and total flavonoids content decreased by 91.1% and 97.5%, respectively. T-SOD activity increased by 33.33%.The scavenging ability of fermented beverage on superoxide anion free radicals was enhanced, and the scavenging ability on DPPH free radicals, hydroxyl free radicals, and ABTS free radicals was weakened. However, the T-AOC increased from 4.15 ± 0.81 to 8.43 ± 0.27 U/mL. Fermented beverage shows antibacterial activity against four pathogens. The minimum inhibitory concentration (MIC) values of Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 6538 were 0.05 mL/mL and 0.025 mL/mL, respectively, and the minimum bactericidal concentration (MBC) were both 0.2 mL/mL. It was observed by fluorescence microscope that the green fluorescence area of the two biofilms is greatly reduced after being treated with fermented beverage. Sensory analysis results show that the average scores of fermented beverage in color, appearance and taste were increased. The overall impression and flavor were decreased. Conclusion These results demonstrated that strawberry fermented beverage has potential benefits such as an antioxidant, antibacterial, and anti-biofilm formation, providing the potential for the fermented beverage to become promising candidates for natural antioxidants, antibacterial agents and anti-biofilm agents.

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Section: Resultsmentioning

confidence: 99%

Evaluation of a strawberry fermented beverage with potential health benefits

Zhao

Wu²,

Chen

et al. 2021

PeerJ

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“…The choice for the use of this NOX enzyme from Lactobacillus sanfranciscensis was born out of the literature reports; in that, it gives the best protein-ligand binding geometries that play a key role in generating ROS; particularly superoxide anion, which is the precursor of most other ROS, and contains diverse amino acids in its catalytic site. [25][26][27][28] The enzyme structure was prepared by deleting water molecules and adding polar hydrogen as well as various charges using AutoDock Tool: as performed by Morris et al 29…”

Section: Selection Of Receptor and Preparationmentioning

confidence: 99%

In Silico Analysis of Antioxidant Phytochemicals with Potential NADPH Oxidase Inhibitory Effect

Mhya

Jakwa

Agbo

2022

J Health Sci Med Res

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Objective: NADPH oxidase (NOX) is known to produce reactive oxygen species (ROS) at physiological concentrations. However, it can be over-activated with some disease conditions and produces excess ROS. Several molecules have shown an ability to suppress the enzyme’s over-activity, although some weaknesses have been found. Hence, the attempt to screen phytochemicals, with the aim of finding the most specific and effective NOX inhibitor. Material and Methods: The study was carried-out via an in-silico approach. First, phytochemicals with antioxidant activity, according to the literature review, were selected and downloaded from the PubChem database in SDF files. NOX with PDB: 2CDU was downloaded from the protein databank. Drug-likeness properties and biological activities were predicted using ADMETMESH and the Predict Activity Spectra of Substances (PASS) software. Phytochemical-NOX interactions were performed via molecular docking, whereas, docked conformations and bond residue amino acids were analyzed using Protein-plus software. Results: The result of this study predicted 13 phytochemicals with drug-likeness properties, out of which 9 showed NOX-inhibitory activity. Docking results predicted all of the 9 phytochemicals were capable of interacting with NOX, by binding to at least one amino acid. The reference inhibitor (Apocynin, -8.3 kcal/mol) and some phytochemicals (caffeic, eriodictyol, hesperetin, and morin with ΔG -6.1 to -7.7 kcal/mol) were predicted to have bonded to Ser115, via hydrogen bonding. On the other hand, epicatechin gallate and quercetin with ΔG −8.7 and −8.1 kcal/mol did not bind to Ser115, but rather through other amino acids. Conclusion: This study has led to the prediction of phytochemicals with NOX-inhibitory effects, which could be considered for further study.

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“…Rahmani H. et al developed aequorin-based inhibition assay for direct detection of dopamine in raw samples of blood serum or urine with a detection limit of 53 nM [ 95 ]. The same research group proposed to use a aequorin bioluminescence system for superoxide anion detection [ 96 ]. The assay was based on decarboxylation of coelenterazine to coelenteramide by superoxide anion.…”

Section: Analytical Application Of Ca 2+ -Regulmentioning

confidence: 99%

Coelenterazine-Dependent Luciferases as a Powerful Analytical Tool for Research and Biomedical Applications

Krasitskaya

Bashmakova

Frank

2020

IJMS

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: The functioning of bioluminescent systems in most of the known marine organisms is based on the oxidation reaction of the same substrate—coelenterazine (CTZ), catalyzed by luciferase. Despite the diversity in structures and the functioning mechanisms, these enzymes can be united into a common group called CTZ-dependent luciferases. Among these, there are two sharply different types of the system organization—Ca2+-regulated photoproteins and luciferases themselves that function in accordance with the classical enzyme–substrate kinetics. Along with deep and comprehensive fundamental research on these systems, approaches and methods of their practical use as highly sensitive reporters in analytics have been developed. The research aiming at the creation of artificial luciferases and synthetic CTZ analogues with new unique properties has led to the development of new experimental analytical methods based on them. The commercial availability of many ready-to-use assay systems based on CTZ-dependent luciferases is also important when choosing them by first-time-users. The development of analytical methods based on these bioluminescent systems is currently booming. The bioluminescent systems under consideration were successfully applied in various biological research areas, which confirms them to be a powerful analytical tool. In this review, we consider the main directions, results, and achievements in research involving these luciferases.

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Bioluminescence Detection of Superoxide Anion Using Aequorin

Cited by 16 publications

References 46 publications

Evaluation of a strawberry fermented beverage with potential health benefits

Evaluation of a strawberry fermented beverage with potential health benefits

In Silico Analysis of Antioxidant Phytochemicals with Potential NADPH Oxidase Inhibitory Effect

Coelenterazine-Dependent Luciferases as a Powerful Analytical Tool for Research and Biomedical Applications

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