Bioluminescence and chemiluminescence in drug screening

Roda, Aldo; Guardigli, Massimo; Pasini, Patrizia; Mirasoli, Mara

doi:10.1007/s00216-003-2096-6

Cited by 94 publications

(41 citation statements)

References 39 publications

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“…The basis of their practical application is the principle of assessing the activity of the luminescence system. This is integrally responsive, showing a decrease of luminescence intensity with the appearance in the test environment of chemical toxicants, or the display of toxic properties of the newly synthesized substances and compounds [10,11]. The advantages of this method are speed, high sensitivity and good correlation with results obtained using more complex methods of biotesting [12].…”

Section: Introductionmentioning

confidence: 99%

Toxicity of Sio2, Tio2 and Ceo2 Nanoparticles Evaluated Using the Bioluminescence Assay

D.B¹

2017

GEOMATE

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ABSTRACT:The development of technology and industry has made available a variety of different nanomaterials. Particularly popular in practical use have been silicon dioxide nanoparticles and titanium and cerium dioxides. However, it is necessary to evaluate the biological activity of nanoparticles from the point of view of toxicology. Despite the active use of nanoparticles of silicon dioxide and dioxides of titanium and cerium, little is known about their toxic effects on living organisms. This work presents a study of three various nanoparticles of silicon dioxide and dioxides of titanium and cerium. For investigations of nanoparticles morphology and their contact with E. coli K12 TG1 cells, suspensions alone or previously mixed with nanoparticles, were applied to freshly prepared mica. To assess the activity of the nanoparticles against E. coli K12 TG1 cells, we used a previously described version of bioluminescent analysis. TiO2 particles having smaller dimensions were characterized by a higher toxicity compared to SiO2. These results characterized TiO2 (I) as toxic and confirm the statement that size matters. Metal particles of CeO2 in the studied concentrations of 0.1-0.000195 M did not cause changes in the dynamics of bacterial bioluminescence. The results were confirmed by studies carried out using lux-biosensors: E. coli K12 MG1655 pkatG'::lux (for the detection of peroxide of hydrogen) and E. coli K12 MG1655 psoxS'::lux ( for the detection of superoxide anion)) for the assessment of oxidative damage.

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Section: Introductionmentioning

confidence: 99%

Toxicity of Sio2, Tio2 and Ceo2 Nanoparticles Evaluated Using the Bioluminescence Assay

D.B¹

2017

GEOMATE

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“…Bioluminescence intensity of luminous bacteria reflects various factors to solve a variety of problems [6]. Numerous applications of the bacterial bioassay are known [7][8][9][10][11][12][13]. The advantages of the bioluminescent assays are speed, sensitivity, simplicity, and availability of the devices for toxicity registration [14].…”

Section: Introductionmentioning

confidence: 99%

Influence of ultrahigh frequency irradiation on Photobacterium phosphoreum luxb gene expression

2014

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Continuous increase in the number and the variety of anthropogenic sources of electromagnetic radiation causes a high interest in studying the effects ultrahigh frequency on living organisms. In the present research influence of UHF EMR (15 W, 2.45 GHz) for 5 and 15 min on morphological and genetic peculiarities of Photobacterium phosphoreum colonies was studied. It has been revealed that UHF EMR affected colony growth parameters, induced transcriptional activity of luciferase encoding gene expression and that the effect was depended on exposure duration. The subsequent cultivation of bacteria during a two week period after treatment showed maintaining of the increased luxb mRNA level in irradiated colonies. Opposite bacterial stress responses were detected to UHF EMR and elevated temperature treatments that assumed UHF EMR comprised of not only thermal but specific component of non-thermal nature.

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“…Due to its simplicity, low cost and high sensitivity and selectivity, CL-based detection has become a quite useful detecting tool in flow injection (FI), column LC and in CE systems, making this technique an interesting field of research, such as clinical [4], pharmaceutical and biomedical [5] or environmental and food analysis [6]. Minimal instrumentation is required and because no external light source is needed, the optical system is quite simple.…”

Section: Introductionmentioning

confidence: 99%

Advances and analytical applications in chemiluminescence coupled to capillary electrophoresis

2010

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Advances and analytical applications in chemiluminescence coupled to capillary electrophoresisThe present review presents the state of the art of the developments, key strategies and analytical applications of chemiluminescence detection coupled to CE (CE-CL). Different parts considering the most common CL systems have been included, such as the tris(2,2 0 -bipyridine)ruthenium(II) system, the luminol and derivatives reaction, the peroxyoxalate CL or direct oxidations. New advances in homemade configurations and applications in different fields such as clinical, pharmaceutical, environmental and food analysis have been included. The focus is on studies which appeared from 2000 to the end of 2009.

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Bioluminescence and chemiluminescence in drug screening

Cited by 94 publications

References 39 publications

Toxicity of Sio2, Tio2 and Ceo2 Nanoparticles Evaluated Using the Bioluminescence Assay

Toxicity of Sio2, Tio2 and Ceo2 Nanoparticles Evaluated Using the Bioluminescence Assay

Influence of ultrahigh frequency irradiation on Photobacterium phosphoreum luxb gene expression

Advances and analytical applications in chemiluminescence coupled to capillary electrophoresis

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