Bioluminescence-Activated Deep-Tissue Photodynamic Therapy of Cancer

Kim, Yi Rang; Kim, Seonghoon; Choi, Jin Woo; Choi, Sung Yong; Lee, Sangeun; Kim, Homin; Hahn, Sei Kwang; Koh, Gou Young; Yun, Seok Hyun

doi:10.7150/thno.11520

Cited by 73 publications

(65 citation statements)

References 68 publications

(68 reference statements)

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“…Moreover, the bioluminescence proteins can be genetically encoded [31] to specific cells. The bioluminescence-cavity complexes can be useful for cellular sensing, tagging, and, possibly, photodynamic therapy [32], particularly in tissues at depths not reachable by external optical excitation.…”

Section: Discussionmentioning

confidence: 99%

Whispering-gallery-mode emission from biological luminescent protein microcavity assemblies

Humar

Yun

2017

Optica

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Fluorescence and bioluminescence are widely used to study biological systems from molecular to whole organism level. However, their broadband emission is often a bottleneck for sensitive spectral measurements and multiplexing. To overcome the limitation, the emitters can be coupled with optical cavity modes to generate narrowband spectral features. Here we demonstrate several types of emitter-resonator complexes made of fluorescent or bioluminescent proteins and artificially or naturally formed optical resonators. We engineered cells to express green fluorescent protein (GFP) fused with ABHD5, which binds to oil or lipid droplets supporting whispering gallery modes (WGM). The genetically-integrated complexes feature well-defined WGM spectral peaks. We measured WGM peaks from GFP-coated BaTiO3 beads (2.56 μm in diameter) during mitosis. Finally, we demonstrate cavity-enhanced bioluminescence using luciferase-coated beads and biochemical excitation. The ability to tailor spontaneous emission by cavity resonance inside biological systems should have applications in biological sensing, imaging and cell tagging.

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Section: Discussionmentioning

confidence: 99%

Whispering-gallery-mode emission from biological luminescent protein microcavity assemblies

Humar

Yun

2017

Optica

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“…115 The authors demonstrated that not only the primary tumour but also the metastatic cancer cells in inguinal LNs were significantly reduced in the treated group, highlighting the great potential for BRET-mediated PDT of tumours located in deep tissues which cannot be reached by external optical illumination. 115 …”

Section: Depth Penetrationmentioning

confidence: 95%

Reactive oxygen species generating systems meeting challenges of photodynamic cancer therapy

et al. 2016

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Reactive oxygen species (ROS)-mediated mechanism is the major cause underlying the efficacy of photodynamic therapy (PDT). The PDT procedure is based on the cascade of synergistic effects between light, photosensitizer (PS) and oxygen, which greatly favor the spatiotemporal control of the treatment. This procedure has also evoked several unresolved challenges at different levels including (i) limited penetration depth of light restricts traditional PDT to only superficial tumours; (ii) oxygen reliance deprives PDT treatment of hypoxic tumours; (iii) light could complicate the phototherapeutic outcomes due to the concurrent heat generation; (iv) specific delivery of PSs to sub-cellular organelles for exerting effective toxicity remains an issue; and (v) side effects by undesirable white-light activation and self-catalysation of traditional PSs. Recent advances in nanotechnology and nanomedicine have provided new opportunities to develop ROS-generating systems through photodynamic or non-photodynamic procedures while tackling the challenges of current PDT approaches. In this review, we summarize the current status and discuss the possible opportunities of ROS generation for cancer therapy. We hope this review will spur pre-clinical research and clinical practice for ROS-mediated tumour treatment.

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“…5a The combination of Nluc and other acceptors can be tuned for appropriate study. For future studies, the advantages of Nluc can also be applied to other systems such as deep-tumor photodynamic therapy 18 and deep-tissue protein-protein interaction study in living systems. 7b, 19 …”

Section: Tumor Imaging With Qd-nluc-crgdmentioning

confidence: 99%

Quantum dot–NanoLuc bioluminescence resonance energy transfer enables tumor imaging and lymph node mapping in vivo

et al. 2016

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A small luciferase protein (Nluc) was conjugated to QDs as a bioluminescence resonance energy transfer (BRET) pair. The conjugate showed 76% BRET efficiency and lymph node mapping was successfully performed. cRGD peptide was conjugated to QD-Nluc for tumor targeting. The self-illuminating QD-Nluc showed excellent energy transfer in a living system and offered optimal tumor-to-background ratio (>85).

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Bioluminescence-Activated Deep-Tissue Photodynamic Therapy of Cancer

Cited by 73 publications

References 68 publications

Whispering-gallery-mode emission from biological luminescent protein microcavity assemblies

Whispering-gallery-mode emission from biological luminescent protein microcavity assemblies

Reactive oxygen species generating systems meeting challenges of photodynamic cancer therapy

Quantum dot–NanoLuc bioluminescence resonance energy transfer enables tumor imaging and lymph node mapping in vivo

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